Lactococcus termiticola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae; Lactococcus

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1667 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2R5HJP1|A0A2R5HJP1_9LACT Uncharacterized protein OS=Lactococcus termiticola OX=2169526 GN=NtB2_00644 PE=4 SV=1
MM1 pKa = 7.48TEE3 pKa = 3.77HH4 pKa = 5.78THH6 pKa = 6.42DD7 pKa = 4.22HH8 pKa = 5.83EE9 pKa = 6.48HH10 pKa = 6.72DD11 pKa = 3.8HH12 pKa = 6.93SHH14 pKa = 7.49DD15 pKa = 3.52EE16 pKa = 4.46DD17 pKa = 5.52FITLVDD23 pKa = 4.07DD24 pKa = 4.53NGNEE28 pKa = 3.97SLYY31 pKa = 10.83QILLTIDD38 pKa = 3.34GQEE41 pKa = 4.05EE42 pKa = 4.07FGKK45 pKa = 10.51NYY47 pKa = 9.85VLVMPVEE54 pKa = 4.33AEE56 pKa = 3.62EE57 pKa = 4.66DD58 pKa = 3.6EE59 pKa = 4.44NGEE62 pKa = 4.24VEE64 pKa = 3.89IQAYY68 pKa = 10.62SFTQNEE74 pKa = 4.71DD75 pKa = 3.02GTEE78 pKa = 4.09GDD80 pKa = 4.51LEE82 pKa = 4.94PIPEE86 pKa = 4.19EE87 pKa = 5.31NEE89 pKa = 4.08EE90 pKa = 3.96EE91 pKa = 4.1WNLIEE96 pKa = 4.41DD97 pKa = 4.11TFNAFMEE104 pKa = 4.36QQEE107 pKa = 4.38DD108 pKa = 3.5

Molecular weight: 12.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2R5HG99|A0A2R5HG99_9LACT Uncharacterized protein OS=Lactococcus termiticola OX=2169526 GN=NtB2_01149 PE=4 SV=1
MM1 pKa = 7.28KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 5.97KK9 pKa = 10.04KK10 pKa = 9.24PRR12 pKa = 11.84KK13 pKa = 4.63TTHH16 pKa = 5.89GFRR19 pKa = 11.84SRR21 pKa = 11.84MATKK25 pKa = 9.94NGRR28 pKa = 11.84RR29 pKa = 11.84VLSARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.68GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1667

0

1667

504073

30

1966

302.4

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.741 ± 0.061

0.414 ± 0.015

5.649 ± 0.055

7.141 ± 0.077

4.514 ± 0.048

6.913 ± 0.053

1.784 ± 0.027

6.913 ± 0.063

6.562 ± 0.059

10.254 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.635 ± 0.03

4.218 ± 0.043

3.478 ± 0.036

3.788 ± 0.039

3.98 ± 0.046

6.716 ± 0.145

5.332 ± 0.066

6.658 ± 0.056

0.962 ± 0.022

3.346 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski