Flaviflexus salsibiostraticola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Flaviflexus

Average proteome isoelectric point is 5.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8WUD4|A0A3Q8WUD4_9ACTO Uncharacterized protein OS=Flaviflexus salsibiostraticola OX=1282737 GN=EJO69_09615 PE=4 SV=1
MM1 pKa = 7.34KK2 pKa = 9.07RR3 pKa = 11.84TLAALASLALLLGACSSTAEE23 pKa = 4.19EE24 pKa = 4.41TASDD28 pKa = 3.94TADD31 pKa = 3.47SPVPTQPAPAGLEE44 pKa = 3.79EE45 pKa = 5.3FYY47 pKa = 11.1NQSLEE52 pKa = 3.9WGEE55 pKa = 4.59CMDD58 pKa = 3.86GTSAEE63 pKa = 4.16AEE65 pKa = 4.33CAKK68 pKa = 10.42VRR70 pKa = 11.84VPLDD74 pKa = 3.42YY75 pKa = 10.78EE76 pKa = 4.61DD77 pKa = 4.77PSEE80 pKa = 4.06ATIDD84 pKa = 3.5IAVLRR89 pKa = 11.84IPAGGEE95 pKa = 3.86AQGSLVVNPGGPGASGMDD113 pKa = 3.48MADD116 pKa = 2.9SAGAYY121 pKa = 9.34FSRR124 pKa = 11.84DD125 pKa = 3.11LVEE128 pKa = 5.04SYY130 pKa = 10.88DD131 pKa = 4.22IIGFDD136 pKa = 3.43PRR138 pKa = 11.84GVGEE142 pKa = 4.36SAPVDD147 pKa = 3.73CLPDD151 pKa = 3.69EE152 pKa = 4.46EE153 pKa = 6.24LGDD156 pKa = 4.24LLDD159 pKa = 3.93RR160 pKa = 11.84AYY162 pKa = 10.0PDD164 pKa = 3.63TPEE167 pKa = 5.7GEE169 pKa = 4.39AEE171 pKa = 4.02ALRR174 pKa = 11.84DD175 pKa = 3.58TKK177 pKa = 10.6TIISSCTKK185 pKa = 10.68ASGHH189 pKa = 5.72LLEE192 pKa = 5.38HH193 pKa = 7.05LSTINTARR201 pKa = 11.84DD202 pKa = 3.47MDD204 pKa = 3.85IMRR207 pKa = 11.84AALGEE212 pKa = 3.89PHH214 pKa = 7.28LDD216 pKa = 3.38YY217 pKa = 11.48LGFSYY222 pKa = 8.83GTHH225 pKa = 7.12LGGQYY230 pKa = 11.24ADD232 pKa = 4.62LFPEE236 pKa = 4.07NVGRR240 pKa = 11.84MVLDD244 pKa = 3.96GAIDD248 pKa = 3.71PAISSLDD255 pKa = 3.23SSYY258 pKa = 10.69FQAVGFEE265 pKa = 4.33KK266 pKa = 10.72ALEE269 pKa = 4.52AYY271 pKa = 10.44LEE273 pKa = 4.26YY274 pKa = 10.86CLEE277 pKa = 5.22DD278 pKa = 3.49EE279 pKa = 5.16DD280 pKa = 5.59CPLEE284 pKa = 4.75AGTVDD289 pKa = 3.37EE290 pKa = 5.75AKK292 pKa = 10.75AHH294 pKa = 5.45LQSEE298 pKa = 4.71IEE300 pKa = 4.3ASLEE304 pKa = 4.22SPIPTSIDD312 pKa = 3.3DD313 pKa = 3.77RR314 pKa = 11.84SVNPAIFYY322 pKa = 9.77TGIATTLYY330 pKa = 10.93SEE332 pKa = 4.48DD333 pKa = 2.97TWPFLTEE340 pKa = 3.97ALRR343 pKa = 11.84MAIEE347 pKa = 5.15DD348 pKa = 3.68GDD350 pKa = 4.14GTLLLTFNDD359 pKa = 3.78LMLGRR364 pKa = 11.84DD365 pKa = 3.57TFSGEE370 pKa = 3.94FMDD373 pKa = 5.24NSLEE377 pKa = 4.02ARR379 pKa = 11.84WAINCRR385 pKa = 11.84DD386 pKa = 3.99FPAEE390 pKa = 3.84TNAAAVEE397 pKa = 4.21ANDD400 pKa = 4.08ARR402 pKa = 11.84LNEE405 pKa = 4.55DD406 pKa = 3.89APTFAPFFEE415 pKa = 6.36GGDD418 pKa = 3.56IFCAGWPYY426 pKa = 10.1TADD429 pKa = 3.49EE430 pKa = 4.55VPGPFVAEE438 pKa = 4.32GSNPIVVIGTEE449 pKa = 4.37GDD451 pKa = 3.23PATPYY456 pKa = 9.75EE457 pKa = 4.2ASVNLAEE464 pKa = 4.05QLEE467 pKa = 4.18NGILVTWEE475 pKa = 4.54GEE477 pKa = 3.86GHH479 pKa = 5.39TAYY482 pKa = 8.92GTAGEE487 pKa = 5.09CIDD490 pKa = 4.83DD491 pKa = 3.86AVDD494 pKa = 3.78RR495 pKa = 11.84YY496 pKa = 10.53FIDD499 pKa = 4.71GEE501 pKa = 4.2LPEE504 pKa = 6.71DD505 pKa = 4.49GLRR508 pKa = 11.84CPAA511 pKa = 4.43

Molecular weight: 54.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8ZCP8|A0A3S8ZCP8_9ACTO ABC transporter ATP-binding protein OS=Flaviflexus salsibiostraticola OX=1282737 GN=EJO69_08750 PE=4 SV=1
MM1 pKa = 7.41VKK3 pKa = 9.03RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84KK12 pKa = 9.27RR13 pKa = 11.84SKK15 pKa = 9.5VHH17 pKa = 5.91GFRR20 pKa = 11.84KK21 pKa = 10.02RR22 pKa = 11.84MATRR26 pKa = 11.84AGRR29 pKa = 11.84AILAGRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.89GRR40 pKa = 11.84AKK42 pKa = 10.68LSAA45 pKa = 3.92

Molecular weight: 5.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2276

0

2276

752478

32

3034

330.6

35.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.796 ± 0.068

0.627 ± 0.015

6.321 ± 0.044

6.505 ± 0.047

3.014 ± 0.032

8.708 ± 0.041

2.105 ± 0.027

5.428 ± 0.037

2.401 ± 0.042

9.778 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.232 ± 0.022

2.217 ± 0.028

5.112 ± 0.036

2.781 ± 0.028

7.104 ± 0.052

6.003 ± 0.032

6.08 ± 0.039

8.158 ± 0.044

1.439 ± 0.02

2.189 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski