Oryza rufipogon (Brownbeard rice) (Asian wild rice)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza

Average proteome isoelectric point is 6.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 45598 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E0Q5E9|A0A0E0Q5E9_ORYRU WD_REPEATS_REGION domain-containing protein OS=Oryza rufipogon OX=4529 PE=4 SV=1
MM1 pKa = 7.37CVDD4 pKa = 4.03GEE6 pKa = 4.31RR7 pKa = 11.84LVVPDD12 pKa = 3.89GGGGGGVEE20 pKa = 4.22IAWRR24 pKa = 11.84KK25 pKa = 8.95YY26 pKa = 8.22VLSYY30 pKa = 10.48FADD33 pKa = 4.13GEE35 pKa = 4.45KK36 pKa = 10.86GSSGWVMHH44 pKa = 6.86EE45 pKa = 3.95YY46 pKa = 10.58AITSPADD53 pKa = 3.53LASSAMRR60 pKa = 11.84LYY62 pKa = 10.66RR63 pKa = 11.84IRR65 pKa = 11.84FSGHH69 pKa = 3.62GKK71 pKa = 8.92KK72 pKa = 9.87RR73 pKa = 11.84KK74 pKa = 9.58RR75 pKa = 11.84EE76 pKa = 3.93PDD78 pKa = 3.25SQSAHH83 pKa = 6.89DD84 pKa = 3.42EE85 pKa = 4.12HH86 pKa = 7.96GRR88 pKa = 11.84ARR90 pKa = 11.84CAPQIAMPEE99 pKa = 4.09TALLEE104 pKa = 4.83DD105 pKa = 4.43SAPPPQPVHH114 pKa = 6.54PPAAVVDD121 pKa = 4.53CVCDD125 pKa = 3.63VTDD128 pKa = 3.6QGSSLVFPDD137 pKa = 3.63QPGSIYY143 pKa = 10.52EE144 pKa = 4.67DD145 pKa = 3.72EE146 pKa = 4.64LQSFVPEE153 pKa = 4.15FAARR157 pKa = 11.84NLFVSLRR164 pKa = 11.84EE165 pKa = 3.84GSRR168 pKa = 11.84DD169 pKa = 3.6VVAEE173 pKa = 3.78AALIEE178 pKa = 4.61DD179 pKa = 5.02LALSPQPVPPPAEE192 pKa = 4.15VVNQADD198 pKa = 4.18DD199 pKa = 4.34SDD201 pKa = 4.81GADD204 pKa = 3.28QGCSSVFAALPDD216 pKa = 4.82LIVLPPEE223 pKa = 4.24EE224 pKa = 4.6ACGSGGAAPAPSWASSLDD242 pKa = 3.62NQNDD246 pKa = 3.55DD247 pKa = 3.62APAFFEE253 pKa = 4.94FPEE256 pKa = 4.57SMDD259 pKa = 4.42DD260 pKa = 3.58MVGCFDD266 pKa = 4.6FASMDD271 pKa = 3.77NQSCTSAVSEE281 pKa = 3.99IAVLEE286 pKa = 4.43EE287 pKa = 4.44PFLPPPTMVNHH298 pKa = 7.24DD299 pKa = 4.31NNSVSDD305 pKa = 4.5GADD308 pKa = 3.03QSCFGVGDD316 pKa = 3.51NSTLVFSDD324 pKa = 3.27LTGSIDD330 pKa = 3.71EE331 pKa = 5.59DD332 pKa = 3.78EE333 pKa = 4.85LQSFVPEE340 pKa = 4.49FVSLPQGSCEE350 pKa = 4.15ADD352 pKa = 3.08AEE354 pKa = 4.36ADD356 pKa = 3.56SGGGVAPAQFAEE368 pKa = 4.51FGGPEE373 pKa = 4.6SMDD376 pKa = 3.77DD377 pKa = 3.73PLNFPAEE384 pKa = 3.99ASGGGDD390 pKa = 2.83RR391 pKa = 11.84AAPASSWVSSQDD403 pKa = 3.43NQNDD407 pKa = 3.58EE408 pKa = 4.34APMFFEE414 pKa = 5.49LPEE417 pKa = 4.43SLDD420 pKa = 3.84DD421 pKa = 3.97MVGCFDD427 pKa = 4.37FAAMDD432 pKa = 4.16GQSCTSAVSEE442 pKa = 4.05TALIEE447 pKa = 4.22EE448 pKa = 4.85LVLPPAAMVNHH459 pKa = 7.04HH460 pKa = 7.66DD461 pKa = 5.13DD462 pKa = 3.97SVSDD466 pKa = 3.83IADD469 pKa = 3.88HH470 pKa = 6.34GCSGAVPPPNSEE482 pKa = 4.57VVDD485 pKa = 4.49LPNDD489 pKa = 3.58SVGADD494 pKa = 3.54QSCSGMVDD502 pKa = 3.65DD503 pKa = 5.66SLSGYY508 pKa = 10.42YY509 pKa = 9.38EE510 pKa = 5.1AEE512 pKa = 4.28LKK514 pKa = 10.54DD515 pKa = 3.68ASGGAGSMMSSPDD528 pKa = 3.13KK529 pKa = 10.82QKK531 pKa = 9.8EE532 pKa = 4.1HH533 pKa = 6.69SSSGVMDD540 pKa = 3.76VEE542 pKa = 4.19ATGFGVPDD550 pKa = 3.59SMDD553 pKa = 3.57GLSCIDD559 pKa = 3.93FAEE562 pKa = 4.32TMDD565 pKa = 5.18DD566 pKa = 4.33LSCIDD571 pKa = 3.63FTIDD575 pKa = 5.64DD576 pKa = 4.56EE577 pKa = 6.63LFDD580 pKa = 3.74LWSS583 pKa = 3.43

Molecular weight: 61.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E0QWC7|A0A0E0QWC7_ORYRU Protein kinase domain-containing protein OS=Oryza rufipogon OX=4529 PE=3 SV=1
MM1 pKa = 7.86DD2 pKa = 5.3KK3 pKa = 10.66KK4 pKa = 10.34HH5 pKa = 6.03STACLHH11 pKa = 6.56RR12 pKa = 11.84PTTGRR17 pKa = 11.84RR18 pKa = 11.84GQAGRR23 pKa = 11.84RR24 pKa = 11.84GRR26 pKa = 11.84AAGRR30 pKa = 11.84RR31 pKa = 11.84SALWRR36 pKa = 11.84RR37 pKa = 11.84GRR39 pKa = 11.84TSSPPRR45 pKa = 11.84RR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84PPSPPPPPPRR58 pKa = 11.84SAPAPWRR65 pKa = 11.84TRR67 pKa = 11.84RR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84TAAAAAPPPRR80 pKa = 11.84TRR82 pKa = 11.84TPPRR86 pKa = 11.84PPPAAAGTAGGSRR99 pKa = 11.84SPSGRR104 pKa = 11.84RR105 pKa = 11.84TAAAPRR111 pKa = 11.84RR112 pKa = 11.84RR113 pKa = 11.84TPRR116 pKa = 11.84GTPPPASSTPAAGTGPASSS135 pKa = 3.73

Molecular weight: 14.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37110

8488

45598

18680246

7

6304

409.7

45.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.171 ± 0.018

1.94 ± 0.006

5.388 ± 0.008

5.989 ± 0.013

3.6 ± 0.007

7.43 ± 0.013

2.51 ± 0.005

4.495 ± 0.01

4.836 ± 0.011

9.55 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.437 ± 0.004

3.549 ± 0.009

5.542 ± 0.013

3.397 ± 0.008

6.443 ± 0.013

8.347 ± 0.015

4.805 ± 0.007

6.703 ± 0.009

1.328 ± 0.004

2.536 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski