Verrucomicrobia phage P8625

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3JS85|A0A0E3JS85_9CAUD Tail fiber protein OS=Verrucomicrobia phage P8625 OX=1636271 GN=P8625_22 PE=4 SV=1
MM1 pKa = 7.58PAANLIEE8 pKa = 4.55LYY10 pKa = 10.56NFEE13 pKa = 4.9GNVEE17 pKa = 4.28SAFKK21 pKa = 10.62SWLADD26 pKa = 3.51NQVEE30 pKa = 4.67LYY32 pKa = 8.59QTIDD36 pKa = 3.51FDD38 pKa = 4.94ILPDD42 pKa = 4.08DD43 pKa = 4.43YY44 pKa = 11.35IGAKK48 pKa = 10.24LEE50 pKa = 4.27LGAVTGHH57 pKa = 6.08YY58 pKa = 10.61NPAPGGAATPEE69 pKa = 3.84YY70 pKa = 10.66DD71 pKa = 4.23QYY73 pKa = 11.26TCSLEE78 pKa = 3.73ITIRR82 pKa = 11.84TARR85 pKa = 11.84FDD87 pKa = 3.36EE88 pKa = 4.8SGDD91 pKa = 3.34VTSPLRR97 pKa = 11.84SRR99 pKa = 11.84HH100 pKa = 4.45QALTAAMRR108 pKa = 11.84TWLSISQAKK117 pKa = 10.03GSALEE122 pKa = 4.91GYY124 pKa = 9.83LNYY127 pKa = 10.72YY128 pKa = 10.09AFEE131 pKa = 4.22FLRR134 pKa = 11.84PAGTAHH140 pKa = 5.61SQEE143 pKa = 4.53SEE145 pKa = 3.91FDD147 pKa = 3.39EE148 pKa = 4.68TTLSFDD154 pKa = 3.5GQISVLPSAFPVLL167 pKa = 3.72

Molecular weight: 18.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3M3Z1|A0A0E3M3Z1_9CAUD Uncharacterized protein OS=Verrucomicrobia phage P8625 OX=1636271 GN=P8625_29 PE=4 SV=1
MM1 pKa = 7.45AKK3 pKa = 10.14KK4 pKa = 10.71GFTLDD9 pKa = 4.03DD10 pKa = 4.91AIFQKK15 pKa = 10.56KK16 pKa = 9.34IRR18 pKa = 11.84DD19 pKa = 3.24LAKK22 pKa = 10.21RR23 pKa = 11.84VGVDD27 pKa = 2.66EE28 pKa = 4.58KK29 pKa = 11.54DD30 pKa = 3.67FVRR33 pKa = 11.84EE34 pKa = 3.79QGALLLRR41 pKa = 11.84DD42 pKa = 3.26IAKK45 pKa = 9.96YY46 pKa = 9.69VPPYY50 pKa = 10.6QSFPTGRR57 pKa = 11.84GTSIGSAKK65 pKa = 10.27DD66 pKa = 3.27KK67 pKa = 11.03KK68 pKa = 10.5QGEE71 pKa = 4.15LAIEE75 pKa = 3.86YY76 pKa = 9.8DD77 pKa = 3.26LRR79 pKa = 11.84KK80 pKa = 9.89IFFMPDD86 pKa = 2.46KK87 pKa = 10.46AQVYY91 pKa = 9.08KK92 pKa = 9.83WAKK95 pKa = 8.43DD96 pKa = 3.44TYY98 pKa = 9.02PTGQIYY104 pKa = 10.3RR105 pKa = 11.84GRR107 pKa = 11.84EE108 pKa = 3.6IIGAGVADD116 pKa = 4.2TLEE119 pKa = 4.04KK120 pKa = 9.75MRR122 pKa = 11.84RR123 pKa = 11.84FHH125 pKa = 7.86RR126 pKa = 11.84SQQRR130 pKa = 11.84RR131 pKa = 11.84TGRR134 pKa = 11.84TRR136 pKa = 11.84ALKK139 pKa = 10.4GSEE142 pKa = 4.11QMWVTPANFNKK153 pKa = 10.21YY154 pKa = 9.38LRR156 pKa = 11.84AEE158 pKa = 4.17KK159 pKa = 10.58LRR161 pKa = 11.84VGIAKK166 pKa = 10.43ASVAKK171 pKa = 10.57GMIRR175 pKa = 11.84LNPAAKK181 pKa = 9.53IPAWVRR187 pKa = 11.84RR188 pKa = 11.84NIGNASGNARR198 pKa = 11.84MTKK201 pKa = 10.45LGGGWNAIFSASAFGLQHH219 pKa = 5.69VQQKK223 pKa = 8.33TMSIIKK229 pKa = 10.04RR230 pKa = 11.84ARR232 pKa = 11.84LKK234 pKa = 11.22AMEE237 pKa = 4.33KK238 pKa = 10.23RR239 pKa = 11.84LLFIMKK245 pKa = 10.24DD246 pKa = 3.41NAKK249 pKa = 10.32KK250 pKa = 10.56AGLKK254 pKa = 9.84VRR256 pKa = 4.32

Molecular weight: 28.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

10500

40

644

201.9

22.44

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.924 ± 0.558

1.343 ± 0.222

6.79 ± 0.279

6.905 ± 0.45

3.733 ± 0.218

7.524 ± 0.479

1.629 ± 0.21

6.295 ± 0.343

6.362 ± 0.492

7.057 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.695 ± 0.226

4.4 ± 0.3

3.324 ± 0.259

3.543 ± 0.23

5.705 ± 0.283

6.8 ± 0.464

5.952 ± 0.604

5.162 ± 0.225

1.686 ± 0.148

3.171 ± 0.259
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski