Clostridium sp. CAG:167

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1994 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R5VI72|R5VI72_9CLOT GH43_C domain-containing protein OS=Clostridium sp. CAG:167 OX=1262777 GN=BN512_00873 PE=3 SV=1
MM1 pKa = 7.74AISVDD6 pKa = 3.33NTYY9 pKa = 9.17LTNYY13 pKa = 8.52STDD16 pKa = 3.32ASASAADD23 pKa = 4.18KK24 pKa = 10.95LSSQIQNASTDD35 pKa = 3.79DD36 pKa = 3.74EE37 pKa = 4.48TMEE40 pKa = 4.16ACKK43 pKa = 10.21QFEE46 pKa = 4.64SYY48 pKa = 9.88MIEE51 pKa = 3.84QMYY54 pKa = 11.14KK55 pKa = 10.7NMEE58 pKa = 4.2KK59 pKa = 10.42ASKK62 pKa = 10.23ILTEE66 pKa = 5.4DD67 pKa = 3.74EE68 pKa = 5.46DD69 pKa = 5.34DD70 pKa = 5.41DD71 pKa = 4.49STSSQYY77 pKa = 11.43VDD79 pKa = 3.75MFHH82 pKa = 7.53DD83 pKa = 4.31NYY85 pKa = 10.12IQTIAEE91 pKa = 4.17SMTNSGQSIGLADD104 pKa = 3.64QLYY107 pKa = 10.16NAIKK111 pKa = 10.29KK112 pKa = 10.45NEE114 pKa = 4.02GTQVV118 pKa = 2.88

Molecular weight: 13.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R5W495|R5W495_9CLOT ATP synthase subunit b OS=Clostridium sp. CAG:167 OX=1262777 GN=atpF PE=3 SV=1
MM1 pKa = 7.6KK2 pKa = 8.56MTYY5 pKa = 8.56QPKK8 pKa = 9.32KK9 pKa = 7.54RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.07VHH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTANGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84KK40 pKa = 8.79KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1994

0

1994

630825

31

1613

316.4

35.46

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.262 ± 0.054

1.478 ± 0.022

5.636 ± 0.037

7.482 ± 0.073

3.878 ± 0.039

7.209 ± 0.047

1.732 ± 0.024

6.869 ± 0.054

7.729 ± 0.064

8.601 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.173 ± 0.023

4.053 ± 0.042

3.201 ± 0.028

3.479 ± 0.035

4.294 ± 0.047

5.883 ± 0.052

5.61 ± 0.054

7.406 ± 0.044

0.883 ± 0.021

4.139 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski