Opitutus sp. ER46

Taxonomy: cellular organisms; Bacteria; PVC group; Verrucomicrobia; Opitutae; Opitutales; Opitutaceae; Opitutus; unclassified Opitutus

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4256 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T6CMD5|A0A2T6CMD5_9BACT Uncharacterized protein OS=Opitutus sp. ER46 OX=2161864 GN=DB354_15720 PE=4 SV=1
MM1 pKa = 7.62ANSSDD6 pKa = 3.62RR7 pKa = 11.84TPDD10 pKa = 3.51NVPGRR15 pKa = 11.84YY16 pKa = 8.85YY17 pKa = 11.06VDD19 pKa = 4.07SSCIDD24 pKa = 3.53CDD26 pKa = 3.29QCRR29 pKa = 11.84VLAPDD34 pKa = 3.69VFARR38 pKa = 11.84NPDD41 pKa = 3.31TGLGYY46 pKa = 10.4VKK48 pKa = 10.28RR49 pKa = 11.84QPLTDD54 pKa = 4.2DD55 pKa = 4.66EE56 pKa = 4.47IALAEE61 pKa = 4.05EE62 pKa = 4.66ALASCATGSIGNDD75 pKa = 3.52GAA77 pKa = 4.36

Molecular weight: 8.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T6DE46|A0A2T6DE46_9BACT Ketoacyl-synt_C domain-containing protein OS=Opitutus sp. ER46 OX=2161864 GN=DB354_01140 PE=4 SV=1
MM1 pKa = 7.52KK2 pKa = 8.83PTFRR6 pKa = 11.84PHH8 pKa = 5.64RR9 pKa = 11.84KK10 pKa = 8.98KK11 pKa = 10.38RR12 pKa = 11.84ARR14 pKa = 11.84QIGFRR19 pKa = 11.84ARR21 pKa = 11.84MATRR25 pKa = 11.84GGRR28 pKa = 11.84KK29 pKa = 9.17VINSRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.15GRR39 pKa = 11.84KK40 pKa = 8.98RR41 pKa = 11.84LTVVV45 pKa = 3.08

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4256

0

4256

1660271

33

3181

390.1

42.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.815 ± 0.056

0.885 ± 0.013

4.915 ± 0.024

5.365 ± 0.036

3.818 ± 0.023

8.142 ± 0.036

2.13 ± 0.018

4.188 ± 0.026

2.931 ± 0.032

10.399 ± 0.046

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.832 ± 0.015

2.731 ± 0.029

5.786 ± 0.032

3.373 ± 0.023

7.766 ± 0.044

5.168 ± 0.033

5.918 ± 0.047

7.764 ± 0.033

1.622 ± 0.021

2.452 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski