Enterobacter phage Enc34

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H6WYM8|H6WYM8_9CAUD Uncharacterized protein OS=Enterobacter phage Enc34 OX=1150990 PE=4 SV=1
MM1 pKa = 7.56LPLKK5 pKa = 9.89LFCEE9 pKa = 4.43YY10 pKa = 10.87AAALVDD16 pKa = 3.57GMISHH21 pKa = 7.1RR22 pKa = 11.84PDD24 pKa = 2.7VSTTYY29 pKa = 11.38DD30 pKa = 3.77DD31 pKa = 4.93GNGSAAGDD39 pKa = 3.72VLALILTPAFVVEE52 pKa = 4.71FEE54 pKa = 5.14DD55 pKa = 5.78LISDD59 pKa = 4.51CVLEE63 pKa = 4.37VPKK66 pKa = 11.0VEE68 pKa = 4.37ILFHH72 pKa = 6.76LGGAVVDD79 pKa = 4.52SVDD82 pKa = 5.01CVGCCWTMPTPDD94 pKa = 5.27QIALWSIPVLTEE106 pKa = 3.61YY107 pKa = 7.81TTATADD113 pKa = 4.24RR114 pKa = 11.84ITRR117 pKa = 11.84FNKK120 pKa = 9.57NVMGSSYY127 pKa = 10.73VEE129 pKa = 4.3KK130 pKa = 10.89ANQQ133 pKa = 2.97

Molecular weight: 14.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H6WYM4|H6WYM4_9CAUD Uncharacterized protein OS=Enterobacter phage Enc34 OX=1150990 PE=4 SV=1
MM1 pKa = 7.84LYY3 pKa = 10.73LIMFIGYY10 pKa = 9.45SIIAVYY16 pKa = 9.77IAHH19 pKa = 7.35DD20 pKa = 3.68VRR22 pKa = 11.84HH23 pKa = 5.42VLNRR27 pKa = 11.84CKK29 pKa = 10.59VEE31 pKa = 3.62RR32 pKa = 11.84RR33 pKa = 11.84YY34 pKa = 11.22SFGKK38 pKa = 9.4RR39 pKa = 11.84VIYY42 pKa = 10.36LRR44 pKa = 11.84EE45 pKa = 4.02KK46 pKa = 9.33KK47 pKa = 8.66TGRR50 pKa = 11.84LIGCSNNFFTLLLQGTKK67 pKa = 10.27

Molecular weight: 7.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

19002

60

864

237.5

26.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.131 ± 0.391

1.116 ± 0.116

6.547 ± 0.201

5.852 ± 0.249

3.589 ± 0.152

7.273 ± 0.266

1.6 ± 0.126

5.594 ± 0.154

5.578 ± 0.263

7.52 ± 0.205

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.189 ± 0.133

4.321 ± 0.182

4.547 ± 0.315

3.873 ± 0.259

5.389 ± 0.192

5.81 ± 0.223

6.568 ± 0.298

7.194 ± 0.269

1.679 ± 0.12

3.631 ± 0.176
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski