Deinococcus soli Cha et al. 2016

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae; Deinococcus

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2935 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0F7JS73|A0A0F7JS73_9DEIO DNA mismatch repair protein MutT OS=Deinococcus soli Cha et al. 2016 OX=1309411 GN=SY84_11125 PE=3 SV=1
MM1 pKa = 7.47KK2 pKa = 10.09KK3 pKa = 9.62SHH5 pKa = 7.11LIVLMAALAAGTAAAEE21 pKa = 4.35GTVAGTTIEE30 pKa = 4.21NTASASFQDD39 pKa = 4.0PANAGTTLTSNSNTVSTTVLPLPGFDD65 pKa = 3.57IVYY68 pKa = 10.46NDD70 pKa = 3.36STADD74 pKa = 3.38GNTISTTPILTTGAVPGQKK93 pKa = 8.54VTTGYY98 pKa = 10.81SVLNNGNVTLTVNLAADD115 pKa = 3.78TTGAASGQTVQYY127 pKa = 10.65YY128 pKa = 10.98LDD130 pKa = 4.05ANGDD134 pKa = 3.84GVADD138 pKa = 3.73STTPITSVIVPVDD151 pKa = 4.04DD152 pKa = 4.67PSTPADD158 pKa = 3.51EE159 pKa = 5.45GIVKK163 pKa = 10.01IVQVVTLPTDD173 pKa = 3.54PALITPSSIFGASPEE188 pKa = 4.13GSVTGTAGADD198 pKa = 3.45PLVTPGNGYY207 pKa = 8.48ATGTTVYY214 pKa = 10.7EE215 pKa = 4.42DD216 pKa = 3.35GKK218 pKa = 11.11AVNTDD223 pKa = 3.4LQFVRR228 pKa = 11.84ITVFAPALDD237 pKa = 4.19NNPNTNPSTPVDD249 pKa = 3.55SAGNPITDD257 pKa = 4.04PALVPPSQTVQIPTEE272 pKa = 4.38TTGKK276 pKa = 8.54TGDD279 pKa = 3.62ATPVVPTTGYY289 pKa = 7.7ITPGAPASDD298 pKa = 4.2PTPGGTPIVPNVAGDD313 pKa = 3.51IQIAYY318 pKa = 8.11PKK320 pKa = 10.56ADD322 pKa = 3.54TNTNPDD328 pKa = 3.14TVVFSNNLTNTSGATDD344 pKa = 3.49KK345 pKa = 11.33VQLFPALADD354 pKa = 3.74GTPDD358 pKa = 3.7PAYY361 pKa = 9.28TYY363 pKa = 10.97NAATGTFTNTTTGVVIRR380 pKa = 11.84FLDD383 pKa = 3.98PVTGAVILASTDD395 pKa = 3.43PNNPTTALYY404 pKa = 7.49PTVTVPTGSTVVYY417 pKa = 8.41RR418 pKa = 11.84TEE420 pKa = 3.69VTYY423 pKa = 10.46PDD425 pKa = 4.59PNDD428 pKa = 3.62SAVIAPVTVLIGADD442 pKa = 3.67SLKK445 pKa = 10.97DD446 pKa = 3.35AAIVSDD452 pKa = 3.76STTTNTILPPSAQFGDD468 pKa = 3.57ATGTLGAVSTPAPIQTINPNGTQSGVASPDD498 pKa = 3.25LTDD501 pKa = 3.4ATAVFPMDD509 pKa = 3.38VVNNGQYY516 pKa = 10.5NDD518 pKa = 4.03SYY520 pKa = 9.95TLSGSVTLNLVGGGTVTVPVLYY542 pKa = 9.93YY543 pKa = 10.95APDD546 pKa = 4.01GSLLPRR552 pKa = 11.84LSTDD556 pKa = 3.41PASPDD561 pKa = 3.13YY562 pKa = 10.85TKK564 pKa = 10.97FITPVIAPGTEE575 pKa = 3.78YY576 pKa = 10.79KK577 pKa = 10.35PYY579 pKa = 11.07AVVQVPAGTLTGDD592 pKa = 3.47YY593 pKa = 9.91TVTQNAVGNYY603 pKa = 6.78STIPMTDD610 pKa = 2.98ANNVIRR616 pKa = 11.84VAPDD620 pKa = 3.08GNVAVAKK627 pKa = 9.58FVAKK631 pKa = 10.44SGVTAASNPVNGINNPANFTATGATGAKK659 pKa = 9.42PGDD662 pKa = 4.54DD663 pKa = 2.58ITYY666 pKa = 10.51RR667 pKa = 11.84ITAKK671 pKa = 10.59NNYY674 pKa = 7.0NTTISNFFLSDD685 pKa = 3.42TVPANTLFKK694 pKa = 10.63SIQLLDD700 pKa = 3.44TSGTVISTNVIYY712 pKa = 10.64KK713 pKa = 9.87VGSGAWGTTAPAAGLANPSVIYY735 pKa = 10.3IALDD739 pKa = 3.53ANNDD743 pKa = 4.68LIPDD747 pKa = 4.44ALAPATTLTAEE758 pKa = 4.2FVVTVKK764 pKa = 10.91

Molecular weight: 77.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7JTQ2|A0A0F7JTQ2_9DEIO Peptide ABC transporter permease OS=Deinococcus soli Cha et al. 2016 OX=1309411 GN=SY84_14985 PE=3 SV=1
MM1 pKa = 7.14GASLAVLTAFLGEE14 pKa = 3.95VRR16 pKa = 11.84APAEE20 pKa = 4.22LLVGMIVTCGLAGAFRR36 pKa = 11.84PSWVALRR43 pKa = 11.84VGAGALAVLIAACLLTPVLRR63 pKa = 11.84IPLQSLTLRR72 pKa = 11.84EE73 pKa = 4.37PPVKK77 pKa = 10.42ADD79 pKa = 4.29AIVALGAGVHH89 pKa = 6.0CGSRR93 pKa = 11.84EE94 pKa = 3.99LEE96 pKa = 3.99ASAVARR102 pKa = 11.84LTGALALWRR111 pKa = 11.84AGYY114 pKa = 10.68APVVTVSEE122 pKa = 4.14QSGLIGPRR130 pKa = 11.84DD131 pKa = 4.0CPKK134 pKa = 10.13MSVLEE139 pKa = 4.29TEE141 pKa = 4.65LTRR144 pKa = 11.84ALYY147 pKa = 10.6GPGGPTLVTLKK158 pKa = 10.84NVTTTRR164 pKa = 11.84DD165 pKa = 2.82EE166 pKa = 4.11AARR169 pKa = 11.84VRR171 pKa = 11.84DD172 pKa = 3.7LTRR175 pKa = 11.84ARR177 pKa = 11.84GWTRR181 pKa = 11.84VLLVTSPSHH190 pKa = 5.82SRR192 pKa = 11.84RR193 pKa = 11.84AAALFRR199 pKa = 11.84SQGVTVVSVPAQEE212 pKa = 3.87WRR214 pKa = 11.84FDD216 pKa = 3.54QALTQPSDD224 pKa = 3.17RR225 pKa = 11.84LVALRR230 pKa = 11.84VLLYY234 pKa = 10.61EE235 pKa = 4.04GLSRR239 pKa = 11.84VKK241 pKa = 10.33AALGGTPEE249 pKa = 3.82RR250 pKa = 4.64

Molecular weight: 26.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2935

0

2935

897889

47

3256

305.9

32.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.893 ± 0.061

0.53 ± 0.012

5.52 ± 0.033

5.027 ± 0.037

3.055 ± 0.029

9.347 ± 0.045

2.258 ± 0.033

3.389 ± 0.041

2.273 ± 0.038

11.617 ± 0.071

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.827 ± 0.024

2.327 ± 0.034

5.952 ± 0.042

3.697 ± 0.033

7.524 ± 0.05

4.628 ± 0.033

6.538 ± 0.059

8.012 ± 0.043

1.376 ± 0.021

2.209 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski