Wad Medani virus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Sedoreovirinae; Orbivirus

Average proteome isoelectric point is 7.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 12 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H4M910|A0A0H4M910_9REOV RNA-directed RNA polymerase OS=Wad Medani virus OX=40067 GN=VP1 PE=3 SV=1
MM1 pKa = 7.85DD2 pKa = 5.85AYY4 pKa = 10.27HH5 pKa = 6.97ARR7 pKa = 11.84ALTVLEE13 pKa = 4.25SLSMASEE20 pKa = 4.32ARR22 pKa = 11.84SHH24 pKa = 6.97RR25 pKa = 11.84DD26 pKa = 2.9PVTEE30 pKa = 3.95TTISIFAMRR39 pKa = 11.84FNATSNRR46 pKa = 11.84PITASPFTRR55 pKa = 11.84EE56 pKa = 3.37ARR58 pKa = 11.84RR59 pKa = 11.84NNFYY63 pKa = 10.97AALDD67 pKa = 3.59VTYY70 pKa = 10.51AALNITTDD78 pKa = 4.14FVLPDD83 pKa = 3.44YY84 pKa = 10.74SQNAQTLAILARR96 pKa = 11.84EE97 pKa = 4.1EE98 pKa = 4.36LPYY101 pKa = 10.74TPGSFRR107 pKa = 11.84RR108 pKa = 11.84MLRR111 pKa = 11.84IRR113 pKa = 11.84EE114 pKa = 4.27CTEE117 pKa = 3.23GAANVRR123 pKa = 11.84EE124 pKa = 4.21EE125 pKa = 4.21VDD127 pKa = 4.24PYY129 pKa = 9.53TQYY132 pKa = 11.25QIVVQTGCDD141 pKa = 4.0LDD143 pKa = 3.89TGAAGCAVFANSPNQLQVTIEE164 pKa = 3.87AGRR167 pKa = 11.84EE168 pKa = 3.57VDD170 pKa = 2.98ITADD174 pKa = 4.17LFPQDD179 pKa = 3.88RR180 pKa = 11.84EE181 pKa = 4.32VIAIEE186 pKa = 3.98VTVQILSHH194 pKa = 6.68AFHH197 pKa = 6.98NGAVMAHH204 pKa = 5.26EE205 pKa = 5.07RR206 pKa = 11.84ALEE209 pKa = 3.89MAIDD213 pKa = 4.31GMPLALGTRR222 pKa = 11.84VTVSVGSRR230 pKa = 11.84VTVANRR236 pKa = 11.84GVQNRR241 pKa = 11.84GIFVVTCHH249 pKa = 6.48RR250 pKa = 11.84FWVPDD255 pKa = 4.17PPRR258 pKa = 11.84IIYY261 pKa = 8.12DD262 pKa = 3.49TMEE265 pKa = 5.34ADD267 pKa = 2.97ILAVYY272 pKa = 8.44TYY274 pKa = 10.98KK275 pKa = 11.07DD276 pKa = 3.88RR277 pKa = 11.84VWDD280 pKa = 3.83ALRR283 pKa = 11.84AYY285 pKa = 10.11VLAAVGLPEE294 pKa = 4.06RR295 pKa = 11.84HH296 pKa = 6.08FPLTPVANPRR306 pKa = 11.84QVLAIALLSRR316 pKa = 11.84LFDD319 pKa = 3.86VYY321 pKa = 10.91CATHH325 pKa = 7.17PEE327 pKa = 4.21LALPMAVPGGALAQRR342 pKa = 11.84LAAALQVFRR351 pKa = 11.84DD352 pKa = 3.73ARR354 pKa = 3.73

Molecular weight: 39.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H4MK95|A0A0H4MK95_9REOV NS4 protein OS=Wad Medani virus OX=40067 GN=NS4 PE=4 SV=1
MM1 pKa = 7.19MGSTMSQVTRR11 pKa = 11.84GALRR15 pKa = 11.84KK16 pKa = 9.37IEE18 pKa = 3.96RR19 pKa = 11.84RR20 pKa = 11.84LDD22 pKa = 3.98GIEE25 pKa = 3.93NFFEE29 pKa = 4.77NIHH32 pKa = 6.44PEE34 pKa = 4.35GEE36 pKa = 4.25GMAGEE41 pKa = 4.07IAEE44 pKa = 4.4NVPVEE49 pKa = 4.57DD50 pKa = 4.15PLALEE55 pKa = 4.5VLTSLEE61 pKa = 4.04WAVDD65 pKa = 3.54QMKK68 pKa = 10.18EE69 pKa = 3.65MCPRR73 pKa = 11.84IILNTGIRR81 pKa = 11.84VLEE84 pKa = 4.24KK85 pKa = 10.58RR86 pKa = 11.84PEE88 pKa = 4.17TPVRR92 pKa = 11.84MIHH95 pKa = 5.63VLAEE99 pKa = 3.95IRR101 pKa = 11.84WRR103 pKa = 11.84RR104 pKa = 11.84VMWTMLSIWLRR115 pKa = 11.84RR116 pKa = 11.84WIRR119 pKa = 11.84TPAGAYY125 pKa = 9.99LPSQNQLWVRR135 pKa = 11.84CARR138 pKa = 11.84ADD140 pKa = 3.33RR141 pKa = 11.84FMFSCLGIRR150 pKa = 11.84NHH152 pKa = 5.67VMEE155 pKa = 5.43MYY157 pKa = 10.68AIMSVRR163 pKa = 11.84RR164 pKa = 11.84CLKK167 pKa = 10.58LSDD170 pKa = 3.27CRR172 pKa = 11.84RR173 pKa = 11.84RR174 pKa = 11.84RR175 pKa = 11.84RR176 pKa = 11.84LRR178 pKa = 11.84RR179 pKa = 11.84KK180 pKa = 7.27MRR182 pKa = 11.84RR183 pKa = 11.84LIGEE187 pKa = 4.31SNN189 pKa = 3.09

Molecular weight: 22.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

12

0

12

6156

189

1303

513.0

57.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.006 ± 0.672

1.413 ± 0.323

5.799 ± 0.372

5.491 ± 0.291

3.46 ± 0.339

5.962 ± 0.315

2.648 ± 0.201

4.906 ± 0.337

3.054 ± 0.404

9.649 ± 0.438

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.891 ± 0.226

2.875 ± 0.141

5.75 ± 0.396

3.265 ± 0.246

8.707 ± 0.406

7.001 ± 0.569

5.962 ± 0.382

6.774 ± 0.361

1.381 ± 0.235

3.005 ± 0.298
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski