Nitrosomonas sp. Nm84

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Nitrosomonadaceae; Nitrosomonas; unclassified Nitrosomonas

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3167 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2V3W3H2|A0A2V3W3H2_9PROT Uncharacterized protein OS=Nitrosomonas sp. Nm84 OX=200124 GN=C8R34_11047 PE=4 SV=1
MM1 pKa = 7.74AIINGTSSSEE11 pKa = 4.36TVNGTTSDD19 pKa = 3.55DD20 pKa = 4.16TINGLGGSDD29 pKa = 3.67TLIGDD34 pKa = 4.75LGDD37 pKa = 3.61DD38 pKa = 3.67TYY40 pKa = 10.82EE41 pKa = 3.85YY42 pKa = 11.25SGIFSTTTLYY52 pKa = 10.29PYY54 pKa = 10.42TDD56 pKa = 3.13IVTEE60 pKa = 4.1LAGSGSDD67 pKa = 3.71TIHH70 pKa = 7.12ILTGSNPGDD79 pKa = 3.38IPASAVRR86 pKa = 11.84VQGGSASDD94 pKa = 4.65PNALWIIADD103 pKa = 4.55GYY105 pKa = 10.28GKK107 pKa = 10.16IYY109 pKa = 10.59LGNQLTAANVEE120 pKa = 4.27SLKK123 pKa = 10.6IGNNAPISLTSGINMTGSSSGEE145 pKa = 4.26TINGSAFNDD154 pKa = 4.37TINGMGGSDD163 pKa = 3.58TLIGGLGDD171 pKa = 3.49DD172 pKa = 3.9TYY174 pKa = 10.33EE175 pKa = 3.87YY176 pKa = 11.25SGIFSTTTLYY186 pKa = 10.29PYY188 pKa = 10.42TDD190 pKa = 3.13IVTEE194 pKa = 4.1LAGSGSDD201 pKa = 3.71TIHH204 pKa = 7.12ILTGSNPGDD213 pKa = 3.38IPASAVRR220 pKa = 11.84VQGGSTSDD228 pKa = 4.16PNSLWIIADD237 pKa = 4.4GYY239 pKa = 10.28GKK241 pKa = 10.16IYY243 pKa = 10.59LGNQLTAANVEE254 pKa = 4.27SLKK257 pKa = 10.6IGNNAPISLEE267 pKa = 3.79WLQIATGTSISLTSGLPMTGNINIQTISGSAYY299 pKa = 10.37NDD301 pKa = 3.49IINGKK306 pKa = 9.3GGNDD310 pKa = 3.43TLIGNLGNDD319 pKa = 3.52TYY321 pKa = 10.96IIGNAGVTITEE332 pKa = 4.38KK333 pKa = 10.92LNEE336 pKa = 4.23GTDD339 pKa = 3.79TVKK342 pKa = 11.01SSISYY347 pKa = 9.69ILPANVEE354 pKa = 4.14NLTLTGTLAINGTGNGSNNKK374 pKa = 8.47LTGNSAANQLKK385 pKa = 10.82GNGGNDD391 pKa = 3.5TLDD394 pKa = 3.39GKK396 pKa = 10.75AGNNTLTGGAGQDD409 pKa = 3.57TFKK412 pKa = 10.38LTSAGHH418 pKa = 6.49IDD420 pKa = 4.99AITDD424 pKa = 4.17FIAADD429 pKa = 3.56DD430 pKa = 4.5TIQLEE435 pKa = 4.4NAVFTALTTPGTLAAGQLKK454 pKa = 10.27IGTQALDD461 pKa = 3.49ANDD464 pKa = 3.21FVVYY468 pKa = 11.0NNVTGALLYY477 pKa = 10.93DD478 pKa = 4.48ADD480 pKa = 4.12ASGAAAAVQIATLSAGLALTNADD503 pKa = 3.47FVVII507 pKa = 4.58

Molecular weight: 51.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2V3WE06|A0A2V3WE06_9PROT Glutamate-1-semialdehyde 2 1-aminomutase OS=Nitrosomonas sp. Nm84 OX=200124 GN=hemL PE=3 SV=1
MM1 pKa = 8.08RR2 pKa = 11.84LRR4 pKa = 11.84SLCKK8 pKa = 10.08QRR10 pKa = 11.84VDD12 pKa = 3.25YY13 pKa = 11.1SDD15 pKa = 3.42AVRR18 pKa = 11.84FVKK21 pKa = 10.18KK22 pKa = 10.13CRR24 pKa = 11.84LIILAASIGQMMFDD38 pKa = 3.8VTRR41 pKa = 11.84EE42 pKa = 3.91MIALNRR48 pKa = 11.84FNLSFYY54 pKa = 10.87CRR56 pKa = 3.58

Molecular weight: 6.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3167

0

3167

995109

27

3778

314.2

34.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.975 ± 0.052

0.952 ± 0.016

5.547 ± 0.054

5.686 ± 0.043

3.984 ± 0.028

7.09 ± 0.076

2.456 ± 0.028

6.951 ± 0.039

4.804 ± 0.053

10.328 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.023

4.405 ± 0.046

4.155 ± 0.034

4.376 ± 0.038

5.214 ± 0.045

6.356 ± 0.047

5.602 ± 0.049

6.556 ± 0.04

1.231 ± 0.019

2.883 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski