Amycolatopsis sacchari

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia;

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7365 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I3XEL4|A0A1I3XEL4_9PSEU Uncharacterized protein OS=Amycolatopsis sacchari OX=115433 GN=SAMN05421835_11557 PE=4 SV=1
MM1 pKa = 7.65SNPRR5 pKa = 11.84YY6 pKa = 9.98LGLSPYY12 pKa = 10.48LYY14 pKa = 9.74YY15 pKa = 10.64TDD17 pKa = 3.42ATEE20 pKa = 4.01ALEE23 pKa = 3.84WLVRR27 pKa = 11.84VFGFTEE33 pKa = 4.08RR34 pKa = 11.84VRR36 pKa = 11.84YY37 pKa = 9.66VDD39 pKa = 3.57GSGQVFQATVAAGDD53 pKa = 3.9AEE55 pKa = 4.34IVLAGVGTDD64 pKa = 3.17YY65 pKa = 11.29WEE67 pKa = 4.54GKK69 pKa = 10.18GVDD72 pKa = 3.58RR73 pKa = 11.84PVGQLNVIYY82 pKa = 10.73VDD84 pKa = 4.88DD85 pKa = 5.68ADD87 pKa = 3.65AQYY90 pKa = 11.8DD91 pKa = 4.08RR92 pKa = 11.84VCAALGEE99 pKa = 4.72GCDD102 pKa = 3.65VSPPQDD108 pKa = 3.25QPYY111 pKa = 8.16GARR114 pKa = 11.84VFTVADD120 pKa = 3.03IGGNSWTFWQQISDD134 pKa = 4.05TADD137 pKa = 3.74LPPGWQEE144 pKa = 3.67IRR146 pKa = 11.84AEE148 pKa = 4.41PPTGDD153 pKa = 3.53HH154 pKa = 7.23DD155 pKa = 4.97LPLGG159 pKa = 3.84

Molecular weight: 17.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I4CNC4|A0A1I4CNC4_9PSEU DUF5667 domain-containing protein OS=Amycolatopsis sacchari OX=115433 GN=SAMN05421835_13633 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.68LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84MQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.69QGKK33 pKa = 8.53

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7365

0

7365

2303454

24

4713

312.8

33.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.362 ± 0.046

0.746 ± 0.008

5.725 ± 0.026

5.899 ± 0.031

2.942 ± 0.017

9.302 ± 0.03

2.212 ± 0.015

3.235 ± 0.018

2.002 ± 0.02

10.742 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.592 ± 0.011

1.852 ± 0.016

6.008 ± 0.024

2.807 ± 0.018

8.102 ± 0.035

4.98 ± 0.022

5.847 ± 0.022

9.104 ± 0.03

1.505 ± 0.012

2.038 ± 0.014
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski