Lactobacillus phage Lpa804

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Harbinvirus; Lactobacillus virus Lpa804

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 105 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S6QAK4|A0A3S6QAK4_9CAUD Uncharacterized protein OS=Lactobacillus phage Lpa804 OX=2059850 GN=Lpa804_120 PE=4 SV=1
MM1 pKa = 7.86GYY3 pKa = 8.59TDD5 pKa = 5.07GVAKK9 pKa = 10.62SMSEE13 pKa = 3.57ASAQGKK19 pKa = 9.29LFLIDD24 pKa = 3.16SFTYY28 pKa = 10.34KK29 pKa = 10.61DD30 pKa = 3.47SNYY33 pKa = 10.31NPDD36 pKa = 4.29DD37 pKa = 4.57LDD39 pKa = 5.63GEE41 pKa = 4.35ALSDD45 pKa = 4.3FLDD48 pKa = 3.43QKK50 pKa = 11.5VDD52 pKa = 3.7LDD54 pKa = 3.73YY55 pKa = 11.38TDD57 pKa = 4.12YY58 pKa = 11.83VEE60 pKa = 5.16LLRR63 pKa = 11.84DD64 pKa = 4.31GYY66 pKa = 11.53AYY68 pKa = 10.46DD69 pKa = 4.27GEE71 pKa = 4.64SNEE74 pKa = 5.11INLTEE79 pKa = 4.27NKK81 pKa = 10.37DD82 pKa = 3.5GFTVNVYY89 pKa = 9.77QSALAFIDD97 pKa = 4.84DD98 pKa = 3.89NSQGGLYY105 pKa = 10.52YY106 pKa = 10.08IFDD109 pKa = 3.81EE110 pKa = 4.54TKK112 pKa = 9.18EE113 pKa = 3.87LLGARR118 pKa = 11.84MGVCGEE124 pKa = 4.7HH125 pKa = 6.55IGSVSVWLDD134 pKa = 3.13TTTPQATLTQGNEE147 pKa = 4.23VYY149 pKa = 10.56HH150 pKa = 5.85LTSEE154 pKa = 4.22DD155 pKa = 3.36TDD157 pKa = 4.89DD158 pKa = 3.79INEE161 pKa = 4.25GLSEE165 pKa = 4.35LFYY168 pKa = 11.16DD169 pKa = 4.45

Molecular weight: 18.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3Q8CQM2|A0A3Q8CQM2_9CAUD ADP-ribose pyrophosphatase OS=Lactobacillus phage Lpa804 OX=2059850 GN=Lpa804_12 PE=4 SV=1
MM1 pKa = 7.99LIRR4 pKa = 11.84EE5 pKa = 4.7EE6 pKa = 4.01RR7 pKa = 11.84TKK9 pKa = 10.57MKK11 pKa = 10.21LYY13 pKa = 10.51KK14 pKa = 10.59LLITIVAGVTLSLVTAYY31 pKa = 9.8KK32 pKa = 10.64ADD34 pKa = 3.58ASRR37 pKa = 11.84PNYY40 pKa = 10.55AYY42 pKa = 9.34TLTRR46 pKa = 11.84TKK48 pKa = 10.6HH49 pKa = 5.24AVKK52 pKa = 9.17LTNTGRR58 pKa = 11.84TEE60 pKa = 3.42NMYY63 pKa = 10.48RR64 pKa = 11.84VTVKK68 pKa = 9.65TGKK71 pKa = 9.47AVKK74 pKa = 8.97WYY76 pKa = 10.86YY77 pKa = 9.39MALNAKK83 pKa = 9.23QSWTVKK89 pKa = 10.21QPNKK93 pKa = 10.12YY94 pKa = 8.49SVTVRR99 pKa = 11.84RR100 pKa = 11.84VSKK103 pKa = 10.91NDD105 pKa = 3.15EE106 pKa = 4.11KK107 pKa = 11.39RR108 pKa = 11.84NADD111 pKa = 3.58PRR113 pKa = 11.84NHH115 pKa = 5.76FTPVGIRR122 pKa = 11.84STQRR126 pKa = 11.84TLWNRR131 pKa = 3.25

Molecular weight: 15.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

105

0

105

28393

44

1420

270.4

30.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.1 ± 0.273

0.599 ± 0.067

7.294 ± 0.23

5.882 ± 0.242

3.585 ± 0.138

6.237 ± 0.269

1.599 ± 0.096

6.294 ± 0.188

7.928 ± 0.342

8.294 ± 0.231

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.328 ± 0.076

6.871 ± 0.21

3.071 ± 0.153

3.518 ± 0.182

3.691 ± 0.139

7.854 ± 0.41

6.385 ± 0.33

6.583 ± 0.178

0.993 ± 0.082

4.892 ± 0.191
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski