Bacillus phage Hyb2phi3Ts-SPbeta

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Spbetavirus; unclassified Spbetavirus

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 188 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7G9US08|A0A7G9US08_9CAUD Uncharacterized protein OS=Bacillus phage Hyb2phi3Ts-SPbeta OX=2735533 PE=4 SV=1
MM1 pKa = 7.52SNFWVIALNKK11 pKa = 9.7NWATLDD17 pKa = 3.63QVKK20 pKa = 9.61EE21 pKa = 3.73AYY23 pKa = 10.47YY24 pKa = 10.68YY25 pKa = 11.21DD26 pKa = 4.99DD27 pKa = 3.56VTKK30 pKa = 10.94EE31 pKa = 3.94EE32 pKa = 4.32LKK34 pKa = 11.08EE35 pKa = 4.12GVDD38 pKa = 3.73NNLITPEE45 pKa = 3.98QYY47 pKa = 10.35QEE49 pKa = 4.17IVGEE53 pKa = 4.44AYY55 pKa = 9.72TSVTLSTEE63 pKa = 3.68

Molecular weight: 7.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7G9USF9|A0A7G9USF9_9CAUD PDDEXK_5 domain-containing protein OS=Bacillus phage Hyb2phi3Ts-SPbeta OX=2735533 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.55NLICFVSFSVKK13 pKa = 10.32SKK15 pKa = 10.62SDD17 pKa = 3.24KK18 pKa = 10.76ANKK21 pKa = 9.43PNTINNIQAVTIRR34 pKa = 11.84YY35 pKa = 8.57LSSNHH40 pKa = 4.68VLLWQILIYY49 pKa = 9.69SFRR52 pKa = 11.84LLTVEE57 pKa = 4.9IIHH60 pKa = 6.78PRR62 pKa = 11.84TSSGSSKK69 pKa = 10.68SISFCRR75 pKa = 11.84TNSSNSIMARR85 pKa = 11.84KK86 pKa = 9.43RR87 pKa = 11.84SSLGSLLKK95 pKa = 10.84SIFFNLGIPFSFIHH109 pKa = 5.73SRR111 pKa = 11.84KK112 pKa = 9.71GG113 pKa = 3.17

Molecular weight: 12.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

188

0

188

37124

33

2294

197.5

22.69

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.129 ± 0.248

0.97 ± 0.097

6.309 ± 0.12

8.216 ± 0.232

4.307 ± 0.176

5.32 ± 0.157

1.902 ± 0.13

7.391 ± 0.168

9.891 ± 0.175

8.544 ± 0.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.341 ± 0.095

6.368 ± 0.157

2.589 ± 0.109

3.542 ± 0.26

3.868 ± 0.163

6.562 ± 0.27

5.293 ± 0.186

5.961 ± 0.177

0.991 ± 0.071

4.507 ± 0.152
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski