Citrobacter phage phiCFP-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus; Citrobacter virus CFP1

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0E3M152|A0A0E3M152_9CAUD Endolysin OS=Citrobacter phage phiCFP-1 OX=1610508 GN=RU52_00016 PE=3 SV=1
MM1 pKa = 7.62ISTNTTHH8 pKa = 7.45NEE10 pKa = 3.85VTQMAMSNMTYY21 pKa = 11.02SNVFAHH27 pKa = 7.22AYY29 pKa = 9.93EE30 pKa = 4.05MLNEE34 pKa = 4.3NIRR37 pKa = 11.84YY38 pKa = 9.79DD39 pKa = 5.05DD40 pKa = 3.94IRR42 pKa = 11.84DD43 pKa = 3.59TDD45 pKa = 3.81GLGDD49 pKa = 5.34AIHH52 pKa = 6.6EE53 pKa = 4.6AADD56 pKa = 3.63SAVPHH61 pKa = 6.4YY62 pKa = 10.5YY63 pKa = 10.65SDD65 pKa = 3.44IFSVMASDD73 pKa = 5.8GIDD76 pKa = 4.0LEE78 pKa = 4.94FEE80 pKa = 4.75DD81 pKa = 5.02SGLMPDD87 pKa = 4.21TKK89 pKa = 10.95DD90 pKa = 3.3VTCILQARR98 pKa = 11.84IYY100 pKa = 9.36EE101 pKa = 4.14QLTIDD106 pKa = 3.75LWEE109 pKa = 4.32KK110 pKa = 11.36AEE112 pKa = 4.51DD113 pKa = 3.81LLNEE117 pKa = 3.94YY118 pKa = 10.53LEE120 pKa = 4.67EE121 pKa = 5.07IEE123 pKa = 4.4EE124 pKa = 4.2EE125 pKa = 4.18EE126 pKa = 4.53EE127 pKa = 3.94EE128 pKa = 4.41VEE130 pKa = 4.12EE131 pKa = 5.49DD132 pKa = 3.84EE133 pKa = 4.93EE134 pKa = 4.44

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0E3JTP3|A0A0E3JTP3_9CAUD Single-stranded DNA-binding protein OS=Citrobacter phage phiCFP-1 OX=1610508 GN=RU52_00014 PE=4 SV=1
MM1 pKa = 7.34RR2 pKa = 11.84TNFEE6 pKa = 4.42KK7 pKa = 10.05FTKK10 pKa = 9.84RR11 pKa = 11.84DD12 pKa = 3.49SVVNEE17 pKa = 5.05HH18 pKa = 6.55GEE20 pKa = 3.93QWQEE24 pKa = 3.33RR25 pKa = 11.84RR26 pKa = 11.84DD27 pKa = 3.81RR28 pKa = 11.84MKK30 pKa = 10.57KK31 pKa = 8.51RR32 pKa = 11.84HH33 pKa = 5.08KK34 pKa = 8.08QQRR37 pKa = 11.84GNSQKK42 pKa = 10.68RR43 pKa = 11.84EE44 pKa = 4.04WNN46 pKa = 3.4

Molecular weight: 5.9 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

11421

37

1322

265.6

29.62

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.281 ± 0.408

0.771 ± 0.151

6.409 ± 0.217

7.206 ± 0.394

3.704 ± 0.228

7.583 ± 0.326

1.926 ± 0.177

5.183 ± 0.211

6.567 ± 0.373

8.029 ± 0.327

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.959 ± 0.187

4.684 ± 0.376

3.59 ± 0.215

4.028 ± 0.281

5.621 ± 0.23

5.858 ± 0.309

5.367 ± 0.219

6.593 ± 0.265

1.375 ± 0.182

3.266 ± 0.15
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski