Polyomavirus sp.

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; unclassified Polyomaviridae

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1D8QQF4|A0A1D8QQF4_9POLY Minor capsid protein OS=Polyomavirus sp. OX=36362 PE=3 SV=1
MM1 pKa = 7.43FLLLGMGALLAVFAEE16 pKa = 4.8VIDD19 pKa = 4.21LAAATGLGIDD29 pKa = 5.06AILSAEE35 pKa = 4.64AITTSEE41 pKa = 4.1ALEE44 pKa = 3.88ALITNLVTLEE54 pKa = 4.19GFSEE58 pKa = 4.61AEE60 pKa = 3.94ALTAAGLTAQASAAFAEE77 pKa = 4.77LGSLYY82 pKa = 10.03PQILYY87 pKa = 10.94ALAFDD92 pKa = 4.26SLTVGTAVAAALAPNSFDD110 pKa = 3.7HH111 pKa = 6.35PTPIANLNMALVRR124 pKa = 11.84WVPDD128 pKa = 3.46FDD130 pKa = 4.67FLFPGFRR137 pKa = 11.84HH138 pKa = 6.31LARR141 pKa = 11.84FINYY145 pKa = 9.0IDD147 pKa = 3.73PTNWAPDD154 pKa = 3.44LYY156 pKa = 9.09EE157 pKa = 3.89TLGRR161 pKa = 11.84YY162 pKa = 7.29VWEE165 pKa = 4.31GLQRR169 pKa = 11.84RR170 pKa = 11.84GEE172 pKa = 4.08RR173 pKa = 11.84FIDD176 pKa = 3.68EE177 pKa = 4.02QIGNIEE183 pKa = 4.11HH184 pKa = 6.57VGRR187 pKa = 11.84EE188 pKa = 3.72AAIRR192 pKa = 11.84TVEE195 pKa = 4.17TFTEE199 pKa = 3.83ALARR203 pKa = 11.84YY204 pKa = 9.03FEE206 pKa = 4.13NARR209 pKa = 11.84WAVSYY214 pKa = 10.18IPHH217 pKa = 7.11HH218 pKa = 6.8IYY220 pKa = 10.65SGLEE224 pKa = 3.56KK225 pKa = 10.48YY226 pKa = 7.88YY227 pKa = 10.14TQLPPLNPIQIRR239 pKa = 11.84DD240 pKa = 3.4VSRR243 pKa = 11.84RR244 pKa = 11.84TGLPIPDD251 pKa = 3.35RR252 pKa = 11.84TVGPNGEE259 pKa = 4.34NVKK262 pKa = 10.17SAQYY266 pKa = 10.21VEE268 pKa = 5.1KK269 pKa = 10.43PEE271 pKa = 4.33SPGGAMQRR279 pKa = 11.84STPDD283 pKa = 2.44WMLPLILGLYY293 pKa = 10.19GDD295 pKa = 5.07VSPSWGEE302 pKa = 3.75TLQDD306 pKa = 4.88LEE308 pKa = 4.54NQDD311 pKa = 3.91GPLKK315 pKa = 10.35KK316 pKa = 9.93RR317 pKa = 11.84KK318 pKa = 8.28KK319 pKa = 7.7THH321 pKa = 5.25

Molecular weight:
35.29 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1D8QQF4|A0A1D8QQF4_9POLY Minor capsid protein OS=Polyomavirus sp. OX=36362 PE=3 SV=1
MM1 pKa = 7.66DD2 pKa = 4.87HH3 pKa = 7.31ALTRR7 pKa = 11.84EE8 pKa = 4.04EE9 pKa = 4.51SVKK12 pKa = 10.73LMQLLKK18 pKa = 10.99LPMEE22 pKa = 4.41QYY24 pKa = 11.84GNFPLMRR31 pKa = 11.84RR32 pKa = 11.84AFLEE36 pKa = 3.98QCKK39 pKa = 10.09VLHH42 pKa = 6.76PDD44 pKa = 2.8KK45 pKa = 11.29GGNAEE50 pKa = 4.2LAKK53 pKa = 10.44EE54 pKa = 4.26LISLYY59 pKa = 10.42RR60 pKa = 11.84KK61 pKa = 10.03LEE63 pKa = 3.91AVIPPLNPNDD73 pKa = 3.78SFTTEE78 pKa = 3.77QVGVSDD84 pKa = 3.96SFVFYY89 pKa = 11.01LRR91 pKa = 11.84DD92 pKa = 3.14WSSCLKK98 pKa = 9.28GTEE101 pKa = 3.73VCICLFCLLRR111 pKa = 11.84DD112 pKa = 3.36SHH114 pKa = 6.52RR115 pKa = 11.84RR116 pKa = 11.84RR117 pKa = 11.84GSKK120 pKa = 8.99PKK122 pKa = 10.03CWGSCFCFEE131 pKa = 5.91CYY133 pKa = 9.67IAWFGLEE140 pKa = 3.75YY141 pKa = 10.01TYY143 pKa = 10.64FIAQSWQAIIAKK155 pKa = 10.2LPFSTLNII163 pKa = 3.97

Molecular weight:
18.86 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1497

163

654

374.3

42.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.279 ± 1.714

2.138 ± 0.76

5.411 ± 0.565

7.148 ± 0.477

5.01 ± 0.502

6.279 ± 0.815

1.937 ± 0.203

5.411 ± 0.185

5.611 ± 1.307

10.955 ± 0.76

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.204 ± 0.231

5.478 ± 0.838

5.678 ± 0.799

3.741 ± 0.287

4.876 ± 0.247

5.611 ± 0.445

5.478 ± 0.679

5.812 ± 0.625

1.269 ± 0.33

3.674 ± 0.181

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski