Diachasma alloeum

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Hymenoptera; Apocrita; Parasitoida; Ichneumonoidea; Braconidae; Opiinae;

Average proteome isoelectric point is 7.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 431 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4E0RKC0|A0A4E0RKC0_9HYME Chemosensory protein 5 OS=Diachasma alloeum OX=454923 GN=Csp5 PE=4 SV=1
MM1 pKa = 7.39ARR3 pKa = 11.84HH4 pKa = 5.5VVCCFLLGVAMQALIVSAGRR24 pKa = 11.84PDD26 pKa = 5.82FITDD30 pKa = 3.14DD31 pKa = 3.78MMAMVADD38 pKa = 4.74DD39 pKa = 4.1KK40 pKa = 11.13ARR42 pKa = 11.84CMGEE46 pKa = 3.6HH47 pKa = 6.38GTTEE51 pKa = 4.02TLIDD55 pKa = 3.9EE56 pKa = 4.89VNNGALPNDD65 pKa = 4.06RR66 pKa = 11.84ALTCYY71 pKa = 9.58MDD73 pKa = 4.11CLFAAFGVIDD83 pKa = 4.03EE84 pKa = 5.28GEE86 pKa = 4.61LEE88 pKa = 4.04VDD90 pKa = 3.51MLVGFLPDD98 pKa = 3.66HH99 pKa = 6.25MQDD102 pKa = 3.1QARR105 pKa = 11.84EE106 pKa = 3.97LLEE109 pKa = 4.75ACAKK113 pKa = 10.42QPGADD118 pKa = 3.51PCDD121 pKa = 3.44KK122 pKa = 10.61VFNIAKK128 pKa = 9.48CVQAKK133 pKa = 10.18RR134 pKa = 11.84PDD136 pKa = 3.51LWFMII141 pKa = 4.75

Molecular weight: 15.52 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4E0S3T2|A0A4E0S3T2_9HYME DNA helicase OS=Diachasma alloeum OX=454923 GN=RECQ4 PE=3 SV=1
MM1 pKa = 7.56RR2 pKa = 11.84RR3 pKa = 11.84SDD5 pKa = 3.51DD6 pKa = 3.15RR7 pKa = 11.84SRR9 pKa = 11.84EE10 pKa = 3.91YY11 pKa = 10.7RR12 pKa = 11.84DD13 pKa = 3.23SRR15 pKa = 11.84DD16 pKa = 3.48YY17 pKa = 11.43LPEE20 pKa = 4.71DD21 pKa = 3.41RR22 pKa = 11.84LRR24 pKa = 11.84EE25 pKa = 4.08LEE27 pKa = 3.96RR28 pKa = 11.84KK29 pKa = 8.67RR30 pKa = 11.84RR31 pKa = 11.84EE32 pKa = 3.56WRR34 pKa = 11.84IEE36 pKa = 3.59QEE38 pKa = 3.69KK39 pKa = 10.54RR40 pKa = 11.84RR41 pKa = 11.84EE42 pKa = 3.94HH43 pKa = 6.43EE44 pKa = 3.75KK45 pKa = 10.61RR46 pKa = 11.84KK47 pKa = 10.28QKK49 pKa = 9.99MIQEE53 pKa = 4.45WEE55 pKa = 3.94AKK57 pKa = 9.37RR58 pKa = 11.84ARR60 pKa = 11.84EE61 pKa = 4.5LEE63 pKa = 4.06GKK65 pKa = 7.43NQRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84SKK72 pKa = 10.52SRR74 pKa = 11.84SRR76 pKa = 11.84SASPGPSRR84 pKa = 11.84RR85 pKa = 11.84RR86 pKa = 11.84GRR88 pKa = 11.84SRR90 pKa = 11.84SVEE93 pKa = 4.14KK94 pKa = 10.95NSTRR98 pKa = 11.84ASTKK102 pKa = 9.46VPVMSEE108 pKa = 4.1RR109 pKa = 11.84FDD111 pKa = 3.7SASSSNTPLFKK122 pKa = 10.82GMEE125 pKa = 4.1GSKK128 pKa = 10.28ISVSEE133 pKa = 3.93LKK135 pKa = 10.38KK136 pKa = 10.48IKK138 pKa = 10.82VNIQRR143 pKa = 11.84DD144 pKa = 3.29ISKK147 pKa = 10.93AEE149 pKa = 3.95EE150 pKa = 3.93TSEE153 pKa = 4.09LLRR156 pKa = 11.84DD157 pKa = 3.31ITSPDD162 pKa = 3.47EE163 pKa = 3.94IVLKK167 pKa = 10.49RR168 pKa = 11.84RR169 pKa = 11.84EE170 pKa = 4.37GEE172 pKa = 3.8GSKK175 pKa = 10.56PIFEE179 pKa = 4.22RR180 pKa = 11.84EE181 pKa = 3.74EE182 pKa = 4.26LKK184 pKa = 10.24TKK186 pKa = 10.25EE187 pKa = 4.37SNNVDD192 pKa = 3.2VPEE195 pKa = 4.21RR196 pKa = 11.84RR197 pKa = 11.84TVVALDD203 pKa = 3.41EE204 pKa = 4.74FEE206 pKa = 4.54TNKK209 pKa = 9.8NRR211 pKa = 11.84STEE214 pKa = 3.8RR215 pKa = 11.84RR216 pKa = 11.84SRR218 pKa = 11.84SLSSGRR224 pKa = 11.84RR225 pKa = 11.84TSHH228 pKa = 5.62SSRR231 pKa = 11.84RR232 pKa = 11.84ASRR235 pKa = 11.84HH236 pKa = 5.04DD237 pKa = 3.62AASKK241 pKa = 10.67SYY243 pKa = 9.31TEE245 pKa = 3.87RR246 pKa = 11.84HH247 pKa = 6.17DD248 pKa = 4.3RR249 pKa = 11.84GHH251 pKa = 6.48SYY253 pKa = 11.46DD254 pKa = 4.42RR255 pKa = 11.84GSTTNDD261 pKa = 2.82RR262 pKa = 11.84YY263 pKa = 10.59HH264 pKa = 5.87EE265 pKa = 4.21RR266 pKa = 11.84KK267 pKa = 9.78YY268 pKa = 10.29PSSRR272 pKa = 11.84KK273 pKa = 9.36SSPEE277 pKa = 3.3RR278 pKa = 11.84VRR280 pKa = 11.84DD281 pKa = 3.53SRR283 pKa = 11.84SRR285 pKa = 11.84RR286 pKa = 11.84SQDD289 pKa = 2.57RR290 pKa = 11.84NARR293 pKa = 11.84AGSHH297 pKa = 6.62RR298 pKa = 11.84DD299 pKa = 3.38STCRR303 pKa = 11.84EE304 pKa = 3.86YY305 pKa = 11.05QDD307 pKa = 3.63YY308 pKa = 11.06DD309 pKa = 3.95SYY311 pKa = 11.32SHH313 pKa = 6.83PRR315 pKa = 11.84RR316 pKa = 11.84HH317 pKa = 5.65EE318 pKa = 3.75VDD320 pKa = 3.23RR321 pKa = 11.84RR322 pKa = 11.84PAALSYY328 pKa = 11.04AEE330 pKa = 5.41PITFPMYY337 pKa = 10.11YY338 pKa = 10.22DD339 pKa = 3.4NLVRR343 pKa = 11.84PMMMDD348 pKa = 3.7PMMMMRR354 pKa = 11.84TPMPMMRR361 pKa = 11.84GRR363 pKa = 11.84VPAMIPTFRR372 pKa = 11.84PPFRR376 pKa = 11.84PRR378 pKa = 11.84FMPSEE383 pKa = 4.24MFRR386 pKa = 11.84LNGPPTQRR394 pKa = 11.84YY395 pKa = 9.03GRR397 pKa = 11.84MFPP400 pKa = 3.75

Molecular weight: 47.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

428

3

431

172772

9

1591

400.9

45.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.076 ± 0.085

2.084 ± 0.054

4.303 ± 0.084

5.659 ± 0.155

5.563 ± 0.111

4.81 ± 0.066

2.231 ± 0.038

8.086 ± 0.118

5.564 ± 0.118

10.539 ± 0.111

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.248 ± 0.05

4.177 ± 0.068

3.642 ± 0.086

3.459 ± 0.062

5.172 ± 0.093

7.291 ± 0.083

5.952 ± 0.058

6.699 ± 0.077

1.466 ± 0.039

3.979 ± 0.067
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski