Sphaerobacter thermophilus (strain DSM 20745 / S 6022)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; Thermomicrobia; Sphaerobacteridae; Sphaerobacterales; Sphaerobacterineae; Sphaerobacteraceae; Sphaerobacter; Sphaerobacter thermophilus

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3471 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1CAD0|D1CAD0_SPHTD Zeaxanthin epoxidase OS=Sphaerobacter thermophilus (strain DSM 20745 / S 6022) OX=479434 GN=Sthe_3373 PE=4 SV=1
MM1 pKa = 7.42RR2 pKa = 11.84TRR4 pKa = 11.84LVRR7 pKa = 11.84LLVILALMLSTMTSLGFAAAQQAGNPSTSMPGRR40 pKa = 11.84YY41 pKa = 8.6LVALKK46 pKa = 9.53STGDD50 pKa = 3.4ARR52 pKa = 11.84FMQAQITEE60 pKa = 4.05MGATVVQNLAPLNLLVVHH78 pKa = 6.25GTEE81 pKa = 4.55QVKK84 pKa = 9.87QRR86 pKa = 11.84LKK88 pKa = 10.65ADD90 pKa = 3.5PRR92 pKa = 11.84VSGVATDD99 pKa = 4.59RR100 pKa = 11.84IQRR103 pKa = 11.84IVPPEE108 pKa = 4.02LAPDD112 pKa = 3.87MFSSPQAAEE121 pKa = 4.82PIQIDD126 pKa = 4.12LTDD129 pKa = 4.17DD130 pKa = 3.76AQNGEE135 pKa = 4.27ITGDD139 pKa = 3.62PAFDD143 pKa = 4.64LSGLMWNIDD152 pKa = 3.86RR153 pKa = 11.84IEE155 pKa = 5.13APDD158 pKa = 3.2AWEE161 pKa = 4.24TTSGEE166 pKa = 4.15PEE168 pKa = 4.36VLVGVADD175 pKa = 3.82TGLDD179 pKa = 3.53STHH182 pKa = 6.84VDD184 pKa = 3.51LEE186 pKa = 4.7DD187 pKa = 4.69NIHH190 pKa = 5.96SVVDD194 pKa = 3.92FTEE197 pKa = 6.05AEE199 pKa = 4.43DD200 pKa = 4.9PPICDD205 pKa = 2.98TFFGVSDD212 pKa = 3.64QALAAQFGGPADD224 pKa = 4.28GDD226 pKa = 4.28WNGHH230 pKa = 5.07GSWIGGNIAAVLNEE244 pKa = 3.73QGVNGVAPNVKK255 pKa = 9.85LVSLKK260 pKa = 9.75ISQWCGYY267 pKa = 10.28AYY269 pKa = 10.62DD270 pKa = 4.03STILSAFLYY279 pKa = 10.36AASNDD284 pKa = 3.08IDD286 pKa = 4.07VVSLSFGGYY295 pKa = 9.6LDD297 pKa = 5.06RR298 pKa = 11.84SDD300 pKa = 4.61PEE302 pKa = 3.36QDD304 pKa = 4.45LIYY307 pKa = 10.37SQYY310 pKa = 11.28ASAVALARR318 pKa = 11.84SEE320 pKa = 4.08GTVIVAAAGNEE331 pKa = 3.98HH332 pKa = 7.17VEE334 pKa = 4.23IGDD337 pKa = 3.76GGQVLSSGMLTTPGTLLDD355 pKa = 4.7GFLDD359 pKa = 3.84LQGLYY364 pKa = 9.09EE365 pKa = 4.45IPGGIPGVVTVGSTGNMVNEE385 pKa = 4.73PSDD388 pKa = 3.76EE389 pKa = 4.27CPEE392 pKa = 4.15GTTEE396 pKa = 5.71GSLATCKK403 pKa = 10.67PEE405 pKa = 5.42DD406 pKa = 4.95DD407 pKa = 3.41PHH409 pKa = 7.51QPFGAGEE416 pKa = 4.06EE417 pKa = 4.2NQLAYY422 pKa = 10.26YY423 pKa = 10.29SNYY426 pKa = 9.53GPRR429 pKa = 11.84IDD431 pKa = 5.05LVAPGGSRR439 pKa = 11.84MFNLPLWDD447 pKa = 3.81RR448 pKa = 11.84GGTPGFPYY456 pKa = 10.16TIEE459 pKa = 4.32DD460 pKa = 5.15EE461 pKa = 4.37YY462 pKa = 11.19TAWQTFSTTSNWALYY477 pKa = 7.64TAQIPCFLFIGGGFPAGQCYY497 pKa = 7.21TTIQGTSMATPHH509 pKa = 5.71VSAVLALVASANPDD523 pKa = 3.25LRR525 pKa = 11.84GDD527 pKa = 3.59PDD529 pKa = 3.71RR530 pKa = 11.84LVEE533 pKa = 4.2IVKK536 pKa = 8.6EE537 pKa = 4.1TAQPISGNEE546 pKa = 4.01TPPLDD551 pKa = 4.87PSDD554 pKa = 4.13TSPGDD559 pKa = 3.06IGGPSCPTGYY569 pKa = 10.1CHH571 pKa = 7.77LGGDD575 pKa = 4.99PIPDD579 pKa = 3.32EE580 pKa = 4.43DD581 pKa = 4.62AYY583 pKa = 11.0GAGLVDD589 pKa = 3.81AAAAVEE595 pKa = 3.93AAMVEE600 pKa = 4.39DD601 pKa = 4.1EE602 pKa = 5.7AGSQSQPP609 pKa = 3.01

Molecular weight: 63.97 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1C246|D1C246_SPHTD Gas vesicle G OS=Sphaerobacter thermophilus (strain DSM 20745 / S 6022) OX=479434 GN=Sthe_0876 PE=4 SV=1
MM1 pKa = 7.28NRR3 pKa = 11.84RR4 pKa = 11.84SRR6 pKa = 11.84QILALTAAALATWTFPFNQVAAIGWLTVAILILPTPRR43 pKa = 11.84LKK45 pKa = 10.93PEE47 pKa = 3.72VVPRR51 pKa = 11.84QRR53 pKa = 11.84LRR55 pKa = 11.84NFLRR59 pKa = 11.84RR60 pKa = 11.84FRR62 pKa = 4.73

Molecular weight: 7.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3471

0

3471

1135642

31

1776

327.2

35.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.136 ± 0.057

0.679 ± 0.011

5.617 ± 0.032

6.397 ± 0.041

3.135 ± 0.021

8.57 ± 0.038

2.05 ± 0.018

4.973 ± 0.029

1.67 ± 0.022

10.546 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.99 ± 0.017

2.057 ± 0.02

6.036 ± 0.035

3.172 ± 0.025

8.355 ± 0.044

4.511 ± 0.025

5.619 ± 0.036

8.545 ± 0.035

1.466 ± 0.02

2.476 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski