Facklamia sp. 252

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Aerococcaceae; Facklamia; unclassified Facklamia

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1842 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6M0L9P4|A0A6M0L9P4_9LACT Galactose-1-phosphate uridylyltransferase OS=Facklamia sp. 252 OX=2678501 GN=galT PE=3 SV=1
MM1 pKa = 7.26SFSRR5 pKa = 11.84IAKK8 pKa = 10.01VYY10 pKa = 11.02DD11 pKa = 3.39RR12 pKa = 11.84FNDD15 pKa = 3.73LEE17 pKa = 4.89VYY19 pKa = 8.0EE20 pKa = 4.15QWLDD24 pKa = 3.54FTLNSVDD31 pKa = 3.65QQPQKK36 pKa = 11.17VLDD39 pKa = 4.07VACGTGWFTSLLAPFVTSITGMDD62 pKa = 4.01IDD64 pKa = 4.94NEE66 pKa = 4.13MLEE69 pKa = 4.44IARR72 pKa = 11.84SEE74 pKa = 4.32DD75 pKa = 3.19PGQVVQYY82 pKa = 10.08VQGDD86 pKa = 4.03MLSMEE91 pKa = 4.86TFASDD96 pKa = 3.42YY97 pKa = 11.7DD98 pKa = 4.48LVTCYY103 pKa = 10.96ADD105 pKa = 3.31SLCFLEE111 pKa = 5.28NAEE114 pKa = 4.32QVQQAIRR121 pKa = 11.84QMLNRR126 pKa = 11.84LAPGGTLLFDD136 pKa = 3.26VWTPYY141 pKa = 10.58QVTTGFDD148 pKa = 3.13GFSYY152 pKa = 10.66FDD154 pKa = 3.65SDD156 pKa = 3.36EE157 pKa = 4.21TAALLWDD164 pKa = 3.79SAVDD168 pKa = 3.87AEE170 pKa = 4.6TLTMEE175 pKa = 4.59HH176 pKa = 6.74YY177 pKa = 9.88LTVFMQQQDD186 pKa = 3.05GRR188 pKa = 11.84YY189 pKa = 9.07DD190 pKa = 3.59RR191 pKa = 11.84EE192 pKa = 4.03EE193 pKa = 4.14VVLTEE198 pKa = 3.65HH199 pKa = 7.54AYY201 pKa = 9.69PLSVYY206 pKa = 9.82QAAFDD211 pKa = 3.78IDD213 pKa = 3.74EE214 pKa = 4.41VASVEE219 pKa = 4.12VLVNFGEE226 pKa = 4.46AIYY229 pKa = 11.01DD230 pKa = 3.69EE231 pKa = 4.64MTHH234 pKa = 6.25QEE236 pKa = 3.63AEE238 pKa = 3.68RR239 pKa = 11.84WFFRR243 pKa = 11.84VVKK246 pKa = 10.47RR247 pKa = 3.92

Molecular weight: 28.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6M0LBK2|A0A6M0LBK2_9LACT ABC transporter permease subunit OS=Facklamia sp. 252 OX=2678501 GN=GL993_08695 PE=3 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.67QPKK8 pKa = 9.36KK9 pKa = 7.48RR10 pKa = 11.84TRR12 pKa = 11.84KK13 pKa = 9.06KK14 pKa = 8.41VHH16 pKa = 5.85GFRR19 pKa = 11.84KK20 pKa = 10.01RR21 pKa = 11.84MSTKK25 pKa = 9.36NGRR28 pKa = 11.84RR29 pKa = 11.84VLRR32 pKa = 11.84KK33 pKa = 9.23RR34 pKa = 11.84RR35 pKa = 11.84LKK37 pKa = 10.81GRR39 pKa = 11.84AKK41 pKa = 10.68LSAA44 pKa = 3.92

Molecular weight: 5.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1842

0

1842

599143

32

3489

325.3

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.403 ± 0.108

0.563 ± 0.015

5.316 ± 0.059

7.354 ± 0.073

4.282 ± 0.049

6.266 ± 0.061

2.027 ± 0.036

7.771 ± 0.061

6.119 ± 0.068

9.672 ± 0.093

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.686 ± 0.037

4.881 ± 0.042

3.455 ± 0.072

4.55 ± 0.065

3.963 ± 0.043

5.864 ± 0.046

5.84 ± 0.058

7.117 ± 0.052

0.963 ± 0.022

3.907 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski