Sheeppox virus (strain Turkey/TU-V02127) (SPPV)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Capripoxvirus; Sheeppox virus

Average proteome isoelectric point is 7.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 144 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3F2YKM8|A0A3F2YKM8_SHEVT DNA-directed RNA polymerase 147 kDa polypeptide OS=Sheeppox virus (strain Turkey/TU-V02127) OX=654928 GN=SPPV_67 PE=3 SV=1
MM1 pKa = 7.85GIRR4 pKa = 11.84HH5 pKa = 6.22EE6 pKa = 4.43LDD8 pKa = 2.66ILLVSEE14 pKa = 4.47NLALKK19 pKa = 10.39NVEE22 pKa = 4.12LLKK25 pKa = 10.77GDD27 pKa = 4.43SYY29 pKa = 12.1GCTINIKK36 pKa = 10.27VNQQKK41 pKa = 10.67KK42 pKa = 10.15LDD44 pKa = 4.22FIIILRR50 pKa = 11.84PDD52 pKa = 2.84WTEE55 pKa = 3.44VRR57 pKa = 11.84NVKK60 pKa = 9.82KK61 pKa = 10.75INMVCNGVVIDD72 pKa = 3.58TTLIKK77 pKa = 10.49KK78 pKa = 9.91SFYY81 pKa = 10.33EE82 pKa = 4.01EE83 pKa = 4.87VYY85 pKa = 10.38SSSVTVFKK93 pKa = 10.43NTTVEE98 pKa = 4.33FFSDD102 pKa = 3.19TSKK105 pKa = 10.85KK106 pKa = 10.46YY107 pKa = 9.94KK108 pKa = 10.15EE109 pKa = 4.37EE110 pKa = 4.05YY111 pKa = 10.06PIVNINTIKK120 pKa = 10.38RR121 pKa = 11.84YY122 pKa = 10.11YY123 pKa = 9.2EE124 pKa = 3.97IKK126 pKa = 10.07DD127 pKa = 3.45SRR129 pKa = 11.84MSCINFEE136 pKa = 4.51SPISDD141 pKa = 3.47YY142 pKa = 11.72DD143 pKa = 3.41QVNYY147 pKa = 10.65LKK149 pKa = 10.6DD150 pKa = 3.78YY151 pKa = 11.29INISNDD157 pKa = 2.8YY158 pKa = 9.69YY159 pKa = 11.41LYY161 pKa = 10.45DD162 pKa = 3.94ACDD165 pKa = 3.51YY166 pKa = 11.05CIISSDD172 pKa = 5.26DD173 pKa = 5.05DD174 pKa = 4.69DD175 pKa = 7.24DD176 pKa = 6.07NDD178 pKa = 3.63NTDD181 pKa = 3.48NDD183 pKa = 4.02EE184 pKa = 4.67EE185 pKa = 5.29DD186 pKa = 5.46DD187 pKa = 4.1EE188 pKa = 5.44VNDD191 pKa = 5.02IEE193 pKa = 5.91DD194 pKa = 4.35DD195 pKa = 4.09YY196 pKa = 11.89EE197 pKa = 3.93

Molecular weight: 23.11 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3F2YKK4|A0A3F2YKK4_SHEVT Uncharacterized protein OS=Sheeppox virus (strain Turkey/TU-V02127) OX=654928 GN=SPPV_43 PE=3 SV=1
MM1 pKa = 7.49ALSTRR6 pKa = 11.84EE7 pKa = 4.14TFSLITVTLLAIFMIVSGSALLFKK31 pKa = 10.82SFAPHH36 pKa = 6.26RR37 pKa = 11.84FIMARR42 pKa = 11.84SLTFDD47 pKa = 4.34KK48 pKa = 10.74ILSFLEE54 pKa = 3.83YY55 pKa = 9.96AAIFIFVPGTVSLYY69 pKa = 9.95SAYY72 pKa = 9.91IKK74 pKa = 9.9TLFKK78 pKa = 11.05

Molecular weight: 8.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

144

0

144

45854

51

2026

318.4

37.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.314 ± 0.121

1.947 ± 0.105

5.884 ± 0.12

5.537 ± 0.119

5.858 ± 0.158

3.389 ± 0.12

1.566 ± 0.063

11.118 ± 0.232

10.025 ± 0.204

9.011 ± 0.179

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.161 ± 0.073

8.88 ± 0.201

2.724 ± 0.095

1.841 ± 0.081

2.861 ± 0.107

8.148 ± 0.15

5.177 ± 0.161

5.463 ± 0.104

0.628 ± 0.046

5.467 ± 0.118
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski