Beihai sobemo-like virus 9

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KEA9|A0A1L3KEA9_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 9 OX=1922706 PE=4 SV=1
MM1 pKa = 7.61AVTIEE6 pKa = 4.26ITDD9 pKa = 3.68DD10 pKa = 3.73MIAQAVLYY18 pKa = 9.37SKK20 pKa = 11.23YY21 pKa = 10.05GGAVGAVMIAIRR33 pKa = 11.84MGLLRR38 pKa = 11.84FLVGVVYY45 pKa = 10.85NVFGFLGFAATTSVGWWRR63 pKa = 11.84YY64 pKa = 8.88HH65 pKa = 4.63SQIGAKK71 pKa = 9.34EE72 pKa = 3.79LAVCKK77 pKa = 9.98TGFVTYY83 pKa = 10.63GDD85 pKa = 3.37RR86 pKa = 11.84TFYY89 pKa = 10.67RR90 pKa = 11.84DD91 pKa = 3.26PSDD94 pKa = 3.48LVLYY98 pKa = 9.61SVKK101 pKa = 10.16PGRR104 pKa = 11.84ARR106 pKa = 11.84FVEE109 pKa = 4.59GQFVLSDD116 pKa = 3.56YY117 pKa = 11.35VLVPEE122 pKa = 4.32EE123 pKa = 4.25TGTIAEE129 pKa = 4.28SALYY133 pKa = 10.38GSDD136 pKa = 4.15PIATRR141 pKa = 11.84SVAPNDD147 pKa = 3.58YY148 pKa = 8.9VSVYY152 pKa = 10.08VVHH155 pKa = 7.81DD156 pKa = 4.16DD157 pKa = 4.02GYY159 pKa = 10.25HH160 pKa = 5.04YY161 pKa = 10.9LGGGFRR167 pKa = 11.84EE168 pKa = 4.19AAFLITAAHH177 pKa = 7.61AITDD181 pKa = 4.14SLDD184 pKa = 3.13HH185 pKa = 7.16RR186 pKa = 11.84IALSKK191 pKa = 10.63DD192 pKa = 2.92GKK194 pKa = 10.67KK195 pKa = 10.02FYY197 pKa = 10.83HH198 pKa = 6.26PGKK201 pKa = 9.95VIRR204 pKa = 11.84HH205 pKa = 5.78FVNDD209 pKa = 3.9DD210 pKa = 3.41YY211 pKa = 11.99VRR213 pKa = 11.84CTGDD217 pKa = 3.23DD218 pKa = 3.15VGAFEE223 pKa = 6.05LSAADD228 pKa = 3.56WAVLGVRR235 pKa = 11.84SVKK238 pKa = 10.2PSVYY242 pKa = 10.02SATGMSRR249 pKa = 11.84IEE251 pKa = 4.08VFGRR255 pKa = 11.84DD256 pKa = 3.31PVGVLKK262 pKa = 11.04AGVGNLLPPTQKK274 pKa = 10.21QKK276 pKa = 10.55YY277 pKa = 8.38LGVVPHH283 pKa = 6.58SASTMKK289 pKa = 10.41GFSGSPVYY297 pKa = 10.37TIGEE301 pKa = 4.25TGRR304 pKa = 11.84KK305 pKa = 8.73IVGLHH310 pKa = 5.34IAGGTGTEE318 pKa = 3.8NYY320 pKa = 7.1MASVHH325 pKa = 6.56EE326 pKa = 4.05IHH328 pKa = 6.49QLRR331 pKa = 11.84RR332 pKa = 11.84KK333 pKa = 9.93LGLIPEE339 pKa = 4.35VPMVAEE345 pKa = 4.97ASPDD349 pKa = 3.32MKK351 pKa = 10.45HH352 pKa = 6.02RR353 pKa = 11.84QFDD356 pKa = 3.56RR357 pKa = 11.84HH358 pKa = 4.99EE359 pKa = 5.07HH360 pKa = 5.99NDD362 pKa = 3.48LMDD365 pKa = 4.7DD366 pKa = 4.15LSRR369 pKa = 11.84VSKK372 pKa = 10.77HH373 pKa = 5.63GGLLRR378 pKa = 11.84VYY380 pKa = 10.95DD381 pKa = 4.36DD382 pKa = 4.91LAGNDD387 pKa = 3.77YY388 pKa = 11.27AEE390 pKa = 4.66SADD393 pKa = 3.39IPKK396 pKa = 10.64AVGTPVTGLEE406 pKa = 4.4PEE408 pKa = 4.25VSSGSGADD416 pKa = 3.91LVPALAPVPSTGNASAKK433 pKa = 9.66EE434 pKa = 3.97KK435 pKa = 10.49AASEE439 pKa = 4.32VADD442 pKa = 3.61VSTDD446 pKa = 3.13ADD448 pKa = 3.89LSHH451 pKa = 7.44PDD453 pKa = 3.79EE454 pKa = 5.16VPDD457 pKa = 3.96EE458 pKa = 4.44LDD460 pKa = 3.3FSKK463 pKa = 10.82LSEE466 pKa = 4.43EE467 pKa = 4.41EE468 pKa = 4.03PVPPIGVKK476 pKa = 9.82RR477 pKa = 11.84VRR479 pKa = 11.84NKK481 pKa = 10.28KK482 pKa = 9.91VGGIGAFLGTTALAGAVIGKK502 pKa = 9.46VGSAFDD508 pKa = 4.77PKK510 pKa = 10.59WLDD513 pKa = 3.28CTAASLAEE521 pKa = 4.1ASAASYY527 pKa = 10.55HH528 pKa = 7.16AMLNSPNFDD537 pKa = 3.19RR538 pKa = 11.84YY539 pKa = 10.04RR540 pKa = 11.84EE541 pKa = 3.94YY542 pKa = 10.32MSASGVVAADD552 pKa = 3.44QQRR555 pKa = 11.84TMKK558 pKa = 10.75GVDD561 pKa = 3.31GTDD564 pKa = 3.24LARR567 pKa = 11.84LFGTGVSNRR576 pKa = 11.84SRR578 pKa = 11.84KK579 pKa = 9.3KK580 pKa = 9.73ALKK583 pKa = 9.81RR584 pKa = 11.84LPARR588 pKa = 11.84FLEE591 pKa = 4.5VVEE594 pKa = 4.65KK595 pKa = 11.05LGLPVKK601 pKa = 10.48QYY603 pKa = 11.15AQWTLPPAGVEE614 pKa = 4.07AMEE617 pKa = 5.0HH618 pKa = 5.91SLATQLGRR626 pKa = 11.84TCSSCWPEE634 pKa = 3.5EE635 pKa = 3.89TRR637 pKa = 11.84RR638 pKa = 11.84QFANKK643 pKa = 9.93SGPQYY648 pKa = 10.89DD649 pKa = 3.33IFLEE653 pKa = 4.54EE654 pKa = 4.11VARR657 pKa = 11.84YY658 pKa = 6.92PANRR662 pKa = 11.84ADD664 pKa = 3.54AFANVHH670 pKa = 5.71SKK672 pKa = 7.45VTRR675 pKa = 11.84FVLGLDD681 pKa = 3.78GDD683 pKa = 4.54KK684 pKa = 11.1SAGWSQHH691 pKa = 4.84FRR693 pKa = 11.84PGTKK697 pKa = 9.77RR698 pKa = 11.84SWQDD702 pKa = 3.21TEE704 pKa = 4.36GLTLASYY711 pKa = 7.6LTRR714 pKa = 11.84CRR716 pKa = 11.84LLLRR720 pKa = 11.84AAVGPCVMAQMTPSQLIEE738 pKa = 4.86AGLSDD743 pKa = 4.74PRR745 pKa = 11.84TLFIKK750 pKa = 10.67NEE752 pKa = 3.83PHH754 pKa = 6.67SDD756 pKa = 3.3SKK758 pKa = 11.43VADD761 pKa = 3.84GRR763 pKa = 11.84WRR765 pKa = 11.84LIWGASLVDD774 pKa = 3.41VCVASVTCRR783 pKa = 11.84RR784 pKa = 11.84QDD786 pKa = 3.19KK787 pKa = 11.03LDD789 pKa = 3.34IEE791 pKa = 4.79QYY793 pKa = 10.35QGGPVEE799 pKa = 5.43GGHH802 pKa = 5.23QQAAGLGHH810 pKa = 7.13HH811 pKa = 7.27DD812 pKa = 3.56AGISRR817 pKa = 11.84LCVEE821 pKa = 4.85FQRR824 pKa = 11.84LIDD827 pKa = 3.54TGLQVFDD834 pKa = 4.76SDD836 pKa = 3.96ASGWDD841 pKa = 3.09MSVNRR846 pKa = 11.84DD847 pKa = 3.36SLYY850 pKa = 11.05ADD852 pKa = 3.63ALRR855 pKa = 11.84RR856 pKa = 11.84ILLYY860 pKa = 10.19EE861 pKa = 4.1GRR863 pKa = 11.84GKK865 pKa = 10.65AVFEE869 pKa = 3.98RR870 pKa = 11.84LALCEE875 pKa = 3.72AAANSAHH882 pKa = 5.64VVLIGGNLWEE892 pKa = 4.17VLKK895 pKa = 10.86PGITASGILSTTAQNSFIRR914 pKa = 11.84ALLYY918 pKa = 10.2AFVGVRR924 pKa = 11.84HH925 pKa = 5.84SVVAGDD931 pKa = 4.36DD932 pKa = 3.64GAGARR937 pKa = 11.84EE938 pKa = 3.92HH939 pKa = 7.04GYY941 pKa = 10.84DD942 pKa = 3.29HH943 pKa = 6.9VKK945 pKa = 10.75ALAEE949 pKa = 3.99YY950 pKa = 10.22GPEE953 pKa = 3.82EE954 pKa = 4.21KK955 pKa = 10.18QVNLYY960 pKa = 8.82TADD963 pKa = 3.5SGLEE967 pKa = 4.1FTSHH971 pKa = 5.68RR972 pKa = 11.84MRR974 pKa = 11.84RR975 pKa = 11.84GTDD978 pKa = 3.55GTWSAEE984 pKa = 3.57FLNLGKK990 pKa = 10.53ACARR994 pKa = 11.84LAFGDD999 pKa = 4.3TVTADD1004 pKa = 3.41QLAGLMFCVRR1014 pKa = 11.84NNPAEE1019 pKa = 4.33LKK1021 pKa = 9.44TLGDD1025 pKa = 3.56IAEE1028 pKa = 4.18EE1029 pKa = 4.12MGWPIAGVTPAFLPFLDD1046 pKa = 3.85

Molecular weight: 113.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KEA9|A0A1L3KEA9_9VIRU Uncharacterized protein OS=Beihai sobemo-like virus 9 OX=1922706 PE=4 SV=1
MM1 pKa = 7.71ANGKK5 pKa = 8.49RR6 pKa = 11.84VKK8 pKa = 10.52KK9 pKa = 10.55KK10 pKa = 8.84IVKK13 pKa = 9.97RR14 pKa = 11.84KK15 pKa = 8.59GNGRR19 pKa = 11.84RR20 pKa = 11.84STADD24 pKa = 2.92QVQAQGTGRR33 pKa = 11.84APRR36 pKa = 11.84VPFGNGGGVKK46 pKa = 9.03STIPRR51 pKa = 11.84LPASCWDD58 pKa = 3.69AFSTAHH64 pKa = 6.57APLPRR69 pKa = 11.84AVGPYY74 pKa = 8.48TVVRR78 pKa = 11.84TTRR81 pKa = 11.84LITTNSRR88 pKa = 11.84FALVGSFARR97 pKa = 11.84FGGTAGAADD106 pKa = 4.89GVPLWSNICCATEE119 pKa = 3.59AAGGSIGSTAATTFYY134 pKa = 10.49AIPSPVPDD142 pKa = 4.48TLTGDD147 pKa = 3.36AGGGTLCPSAISVQVMGYY165 pKa = 10.88DD166 pKa = 3.64SLQNAHH172 pKa = 5.78GQIAAAVCPVRR183 pKa = 11.84MDD185 pKa = 3.54LRR187 pKa = 11.84GSTRR191 pKa = 11.84SWDD194 pKa = 3.49GVGAQFLAYY203 pKa = 9.74FRR205 pKa = 11.84PRR207 pKa = 11.84LMSAGKK213 pKa = 7.97VTLRR217 pKa = 11.84GVQMDD222 pKa = 3.71SHH224 pKa = 7.04PLSMADD230 pKa = 3.11VSQFRR235 pKa = 11.84GVDD238 pKa = 3.7EE239 pKa = 4.38VPEE242 pKa = 4.13ALNPLGTTPNSWSLADD258 pKa = 5.09APYY261 pKa = 9.03DD262 pKa = 3.93TNGWAPMVVYY272 pKa = 10.55NPSSANLSLLITFEE286 pKa = 3.53WRR288 pKa = 11.84VRR290 pKa = 11.84FDD292 pKa = 3.04ISNPAVASHH301 pKa = 5.58SHH303 pKa = 6.45HH304 pKa = 6.23GVSSDD309 pKa = 3.68VSWEE313 pKa = 3.82KK314 pKa = 10.85HH315 pKa = 4.05IAAATRR321 pKa = 11.84QLPGVIDD328 pKa = 3.56IVEE331 pKa = 4.34KK332 pKa = 10.52VANTGMAVASMLRR345 pKa = 11.84GG346 pKa = 3.37

Molecular weight: 36.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1392

346

1046

696.0

74.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.279 ± 0.533

1.293 ± 0.071

6.034 ± 0.655

4.31 ± 1.196

3.592 ± 0.192

9.986 ± 0.194

2.586 ± 0.261

3.52 ± 0.11

3.951 ± 0.492

8.261 ± 1.018

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.24

2.658 ± 0.51

5.029 ± 0.751

2.73 ± 0.074

6.178 ± 0.218

7.471 ± 0.691

5.675 ± 0.72

8.908 ± 0.024

1.509 ± 0.239

2.945 ± 0.562
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski