Bacillus phage vB_BboS-125

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Elmenteitavirus; Bacillus virus 125

Average proteome isoelectric point is 6.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 80 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BVW9|A0A3G3BVW9_9CAUD AP2 domain protein OS=Bacillus phage vB_BboS-125 OX=2419618 GN=BboS125_00033 PE=4 SV=1
MM1 pKa = 7.9DD2 pKa = 3.89KK3 pKa = 10.91CEE5 pKa = 3.9RR6 pKa = 11.84CEE8 pKa = 3.83EE9 pKa = 4.15VTRR12 pKa = 11.84ITVTSNGVYY21 pKa = 9.96VCNDD25 pKa = 2.88CWIDD29 pKa = 4.05GDD31 pKa = 4.77DD32 pKa = 4.56KK33 pKa = 11.45EE34 pKa = 4.43

Molecular weight: 3.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BVV0|A0A3G3BVV0_9CAUD Uncharacterized protein OS=Bacillus phage vB_BboS-125 OX=2419618 GN=BboS125_00019 PE=4 SV=1
MM1 pKa = 7.12SWKK4 pKa = 10.76SMLYY8 pKa = 10.87KK9 pKa = 9.51MLKK12 pKa = 9.83YY13 pKa = 10.88SNDD16 pKa = 3.02YY17 pKa = 10.79NAIKK21 pKa = 9.93KK22 pKa = 9.92GKK24 pKa = 7.61VGRR27 pKa = 11.84RR28 pKa = 11.84IGRR31 pKa = 11.84RR32 pKa = 11.84VAGKK36 pKa = 8.83ATGRR40 pKa = 11.84LFGKK44 pKa = 9.93LFKK47 pKa = 10.87

Molecular weight: 5.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

80

0

80

17592

30

1178

219.9

24.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.436 ± 0.382

0.483 ± 0.107

5.906 ± 0.221

8.072 ± 0.337

3.223 ± 0.219

7.663 ± 0.288

1.467 ± 0.149

5.52 ± 0.24

7.452 ± 0.479

8.544 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.183 ± 0.214

4.127 ± 0.205

3.757 ± 0.192

4.718 ± 0.218

5.275 ± 0.316

4.269 ± 0.325

5.883 ± 0.304

6.799 ± 0.213

1.336 ± 0.1

3.888 ± 0.225
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski