Chitinophaga extrema

Taxonomy: cellular organisms; Bacteria; FCB group;

Average proteome isoelectric point is 6.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5973 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S1B478|A0A3S1B478_9BACT FtsX-like permease family protein OS=Chitinophaga extrema OX=2485489 GN=ECE50_02535 PE=4 SV=1
MM1 pKa = 7.59ALLVVTFLRR10 pKa = 11.84VDD12 pKa = 4.65FPAVAFLVVDD22 pKa = 4.75FLAVDD27 pKa = 4.15LAEE30 pKa = 4.29VARR33 pKa = 11.84LAVAFLAVAFLAVVFLAVDD52 pKa = 3.89FLAVVFLAVDD62 pKa = 3.94FFAAAFLAAAFFGTLTPSLRR82 pKa = 11.84ASDD85 pKa = 3.93NPIAIACLGLVTFFPFLPLFSLPSCISCITFSTFSCDD122 pKa = 3.12FLEE125 pKa = 4.53YY126 pKa = 10.2FAITEE131 pKa = 4.26DD132 pKa = 4.15LNGG135 pKa = 3.5

Molecular weight: 14.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S1AX72|A0A3S1AX72_9BACT Uncharacterized protein OS=Chitinophaga extrema OX=2485489 GN=ECE50_26125 PE=4 SV=1
MM1 pKa = 7.42SVINCAVTAGLNSPDD16 pKa = 2.82RR17 pKa = 11.84RR18 pKa = 11.84ARR20 pKa = 11.84RR21 pKa = 11.84GVQGNWCHH29 pKa = 6.19KK30 pKa = 10.0GKK32 pKa = 10.92YY33 pKa = 8.76PVLNLFSRR41 pKa = 11.84KK42 pKa = 8.95PFSRR46 pKa = 11.84RR47 pKa = 11.84ADD49 pKa = 3.3QVIQADD55 pKa = 4.15VPVLSLSCC63 pKa = 4.3

Molecular weight: 6.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5973

0

5973

2372467

26

11197

397.2

44.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.581 ± 0.033

0.872 ± 0.013

5.138 ± 0.021

5.267 ± 0.035

4.481 ± 0.023

7.056 ± 0.034

2.105 ± 0.018

6.509 ± 0.024

5.519 ± 0.039

9.685 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.017

4.889 ± 0.032

4.38 ± 0.022

4.263 ± 0.024

4.81 ± 0.023

6.041 ± 0.022

6.124 ± 0.038

6.402 ± 0.026

1.29 ± 0.011

4.154 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski