Roseobacter denitrificans (strain ATCC 33942 / OCh 114) (Erythrobacter sp. (strain OCh 114)) (Roseobacter denitrificans)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Roseobacteraceae; Roseobacter; Roseobacter denitrificans

Average proteome isoelectric point is 6.15

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4118 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q163G5|Q163G5_ROSDO Conserved domain protein OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=RD1_3386 PE=4 SV=1
MM1 pKa = 7.43TEE3 pKa = 3.82YY4 pKa = 11.07GHH6 pKa = 6.55TVGLDD11 pKa = 3.3GSVEE15 pKa = 3.96LSCPVIEE22 pKa = 4.52KK23 pKa = 10.44GKK25 pKa = 9.27YY26 pKa = 8.01VDD28 pKa = 3.69WIEE31 pKa = 4.42RR32 pKa = 11.84IYY34 pKa = 10.38IYY36 pKa = 10.94SPDD39 pKa = 4.22SDD41 pKa = 3.51WEE43 pKa = 4.11AAPDD47 pKa = 3.96TDD49 pKa = 4.3TGPIDD54 pKa = 3.97DD55 pKa = 5.51FEE57 pKa = 4.74IQFSFKK63 pKa = 10.72DD64 pKa = 3.32

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q16AA9|Q16AA9_ROSDO Amino acid ABC transporter permease protein OS=Roseobacter denitrificans (strain ATCC 33942 / OCh 114) OX=375451 GN=RD1_1446 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.81IINARR34 pKa = 11.84RR35 pKa = 11.84AHH37 pKa = 5.43GRR39 pKa = 11.84KK40 pKa = 9.31SLSAA44 pKa = 3.91

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4118

0

4118

1285700

30

6682

312.2

33.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.013 ± 0.044

0.957 ± 0.014

6.178 ± 0.041

5.611 ± 0.034

3.919 ± 0.024

8.428 ± 0.053

2.073 ± 0.022

5.393 ± 0.027

3.298 ± 0.034

9.833 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.846 ± 0.022

2.805 ± 0.028

4.912 ± 0.028

3.469 ± 0.021

6.293 ± 0.045

5.391 ± 0.029

5.682 ± 0.038

7.29 ± 0.037

1.368 ± 0.017

2.243 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski