Actinomyces sp. S4-C9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Actinomycetales; Actinomycetaceae; Actinomyces; unclassified Actinomyces

Average proteome isoelectric point is 6.03

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A095YXG3|A0A095YXG3_9ACTO Sulfide:quinone reductase OS=Actinomyces sp. S4-C9 OX=1219581 GN=HMPREF1628_06880 PE=4 SV=1
MM1 pKa = 7.44SFQVACPADD10 pKa = 4.05LEE12 pKa = 4.65AGQARR17 pKa = 11.84MVEE20 pKa = 4.32LTNEE24 pKa = 4.22AGEE27 pKa = 4.24EE28 pKa = 4.0LPIAIIRR35 pKa = 11.84DD36 pKa = 4.05TVGDD40 pKa = 4.0WYY42 pKa = 10.56AIYY45 pKa = 7.97DD46 pKa = 4.2TCPHH50 pKa = 6.82ADD52 pKa = 3.2VSLSEE57 pKa = 4.37GDD59 pKa = 3.22ISQACVEE66 pKa = 4.57CWAHH70 pKa = 5.82SAEE73 pKa = 4.5INLKK77 pKa = 9.18TGEE80 pKa = 4.0ATLPVTEE87 pKa = 4.37TVRR90 pKa = 11.84TYY92 pKa = 10.16PVKK95 pKa = 11.03LEE97 pKa = 4.08GQNVLVDD104 pKa = 4.4LEE106 pKa = 4.56VEE108 pKa = 4.15

Molecular weight: 11.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A095WTS1|A0A095WTS1_9ACTO DtxR family iron (Metal) dependent repressor OS=Actinomyces sp. S4-C9 OX=1219581 GN=HMPREF1628_06625 PE=3 SV=1
MM1 pKa = 7.62TKK3 pKa = 9.09RR4 pKa = 11.84TYY6 pKa = 10.32QPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84SRR15 pKa = 11.84KK16 pKa = 8.57HH17 pKa = 4.77GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AILANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.51GRR40 pKa = 11.84KK41 pKa = 8.14QLSAA45 pKa = 3.9

Molecular weight: 5.51 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1447

0

1447

507667

38

1836

350.8

38.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.07 ± 0.071

0.628 ± 0.015

5.809 ± 0.06

6.621 ± 0.069

3.345 ± 0.038

8.173 ± 0.049

1.953 ± 0.031

5.38 ± 0.049

3.752 ± 0.041

9.652 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.433 ± 0.033

2.921 ± 0.031

4.754 ± 0.037

3.387 ± 0.034

6.23 ± 0.06

6.022 ± 0.048

5.648 ± 0.046

8.489 ± 0.054

1.399 ± 0.024

2.333 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski