Merremia mosaic virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.26

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q59DM8|Q59DM8_9GEMI Transcriptional activator protein OS=Merremia mosaic virus OX=77813 GN=AC2 PE=3 SV=1
MM1 pKa = 7.31PRR3 pKa = 11.84KK4 pKa = 10.03GSFSIKK10 pKa = 9.74AKK12 pKa = 10.64NYY14 pKa = 8.27FLTYY18 pKa = 8.87PICSLAKK25 pKa = 10.43EE26 pKa = 4.19EE27 pKa = 4.62ALSQIKK33 pKa = 10.08ALHH36 pKa = 5.76TPVNKK41 pKa = 10.18KK42 pKa = 9.94FIKK45 pKa = 9.73ICRR48 pKa = 11.84EE49 pKa = 3.55LHH51 pKa = 7.27DD52 pKa = 4.15NGEE55 pKa = 4.21PHH57 pKa = 6.78LHH59 pKa = 5.96VLIQFEE65 pKa = 4.49GKK67 pKa = 10.13YY68 pKa = 10.33NCTNNRR74 pKa = 11.84FFDD77 pKa = 4.02LVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.25SSSDD99 pKa = 3.11VKK101 pKa = 11.24SYY103 pKa = 10.79IDD105 pKa = 3.8KK106 pKa = 11.28DD107 pKa = 3.26GDD109 pKa = 3.91TIEE112 pKa = 4.27WGQFQIDD119 pKa = 3.41GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSSNDD132 pKa = 3.62TYY134 pKa = 11.93AKK136 pKa = 10.51ALNAASAEE144 pKa = 4.07EE145 pKa = 3.73ALQIIKK151 pKa = 10.28EE152 pKa = 4.13EE153 pKa = 4.13QPQHH157 pKa = 6.73FFLQHH162 pKa = 6.07HH163 pKa = 6.14NLVANATRR171 pKa = 11.84IFQKK175 pKa = 10.63SPEE178 pKa = 4.02PWVPPFQLSSFTNVPDD194 pKa = 5.1EE195 pKa = 4.15MQEE198 pKa = 3.71WADD201 pKa = 3.46NYY203 pKa = 10.45FGRR206 pKa = 11.84GAAARR211 pKa = 11.84ADD213 pKa = 4.06RR214 pKa = 11.84PISIIIEE221 pKa = 4.2GDD223 pKa = 3.24SRR225 pKa = 11.84TGKK228 pKa = 8.52TMWARR233 pKa = 11.84SLGKK237 pKa = 9.59HH238 pKa = 5.79NYY240 pKa = 10.01LSGHH244 pKa = 6.8LDD246 pKa = 3.37FNGRR250 pKa = 11.84VYY252 pKa = 11.32SNDD255 pKa = 2.74VEE257 pKa = 4.62YY258 pKa = 11.36NVIDD262 pKa = 5.26DD263 pKa = 4.49ISPNYY268 pKa = 10.3LKK270 pKa = 10.74LKK272 pKa = 9.13HH273 pKa = 5.7WKK275 pKa = 9.67EE276 pKa = 3.98LIGAQKK282 pKa = 10.57DD283 pKa = 3.42WQSNCKK289 pKa = 9.41YY290 pKa = 10.19GKK292 pKa = 9.27PVQTKK297 pKa = 9.82GGIPSIVLCNPGEE310 pKa = 4.16GASYY314 pKa = 11.32KK315 pKa = 10.91DD316 pKa = 4.51FLDD319 pKa = 3.75KK320 pKa = 11.41DD321 pKa = 3.92EE322 pKa = 4.55NASLRR327 pKa = 11.84AWTIHH332 pKa = 4.71NAKK335 pKa = 10.35FIFLNSPLYY344 pKa = 10.57QSTAQEE350 pKa = 4.05SEE352 pKa = 4.51EE353 pKa = 4.01IQICHH358 pKa = 6.19

Molecular weight: 40.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q9YV57|Q9YV57_9GEMI Capsid protein OS=Merremia mosaic virus OX=77813 GN=AV1 PE=3 SV=2
MM1 pKa = 7.41VKK3 pKa = 9.9RR4 pKa = 11.84DD5 pKa = 3.78APWRR9 pKa = 11.84LMAGTTKK16 pKa = 10.32VSRR19 pKa = 11.84NANFSPRR26 pKa = 11.84GGMGPKK32 pKa = 9.51AAAWVNRR39 pKa = 11.84PMYY42 pKa = 9.63RR43 pKa = 11.84KK44 pKa = 8.95PRR46 pKa = 11.84IYY48 pKa = 9.56RR49 pKa = 11.84TLRR52 pKa = 11.84GPDD55 pKa = 3.31VPKK58 pKa = 10.83GCEE61 pKa = 4.39GPCKK65 pKa = 10.23VQSFEE70 pKa = 3.82QRR72 pKa = 11.84HH73 pKa = 6.26DD74 pKa = 3.16ISHH77 pKa = 5.68VGKK80 pKa = 10.14VICISDD86 pKa = 3.51VTRR89 pKa = 11.84GNGITHH95 pKa = 6.78RR96 pKa = 11.84VGKK99 pKa = 9.44RR100 pKa = 11.84FCVKK104 pKa = 9.92SVYY107 pKa = 10.16ILGKK111 pKa = 9.37IWMDD115 pKa = 3.39EE116 pKa = 4.0NIKK119 pKa = 10.61LKK121 pKa = 10.73NHH123 pKa = 5.95TNSVMFWLIRR133 pKa = 11.84DD134 pKa = 3.59RR135 pKa = 11.84RR136 pKa = 11.84PYY138 pKa = 8.14GTPMDD143 pKa = 4.6FGQVFNMYY151 pKa = 10.66DD152 pKa = 3.86NEE154 pKa = 4.34PSTATVKK161 pKa = 10.85NDD163 pKa = 2.82LRR165 pKa = 11.84DD166 pKa = 3.48RR167 pKa = 11.84FQVMHH172 pKa = 6.98RR173 pKa = 11.84FYY175 pKa = 11.59AKK177 pKa = 9.12VTGGQYY183 pKa = 11.1ASNEE187 pKa = 3.84QALVRR192 pKa = 11.84RR193 pKa = 11.84FWKK196 pKa = 9.41VNNYY200 pKa = 7.92VVYY203 pKa = 10.42NHH205 pKa = 6.0QEE207 pKa = 3.43AGKK210 pKa = 10.41YY211 pKa = 8.39EE212 pKa = 4.09NHH214 pKa = 6.52TEE216 pKa = 3.99NALLLYY222 pKa = 8.57MASTHH227 pKa = 6.41ASNPVYY233 pKa = 9.83ATLKK237 pKa = 9.04IRR239 pKa = 11.84SYY241 pKa = 10.85FYY243 pKa = 11.18DD244 pKa = 3.89SISNN248 pKa = 3.66

Molecular weight: 28.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1430

132

358

238.3

27.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.874 ± 0.684

1.818 ± 0.153

5.175 ± 0.331

4.406 ± 0.481

4.685 ± 0.424

5.105 ± 0.569

4.056 ± 0.421

6.503 ± 0.693

6.294 ± 0.563

6.503 ± 0.54

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.378 ± 0.588

5.874 ± 0.576

4.825 ± 0.266

3.986 ± 0.684

6.713 ± 0.924

9.021 ± 1.067

5.245 ± 0.664

5.804 ± 1.124

1.538 ± 0.201

4.196 ± 0.624
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski