Proteome-pI 2.0 - Proteome Isoelectric Point Database

Beihai narna-like virus 13

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 8.16

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A1L3KI29|A0A1L3KI29_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 13 OX=1922440 PE=4 SV=1

MM1 pKa = 7.55AKK3 pKa = 9.69IFKK6 pKa = 10.5LSIHH10 pKa = 5.82TFFSKK15 pKa = 10.03CTDD18 pKa = 3.09QLLPEE23 pKa = 4.52DD24 pKa = 3.75HH25 pKa = 7.08HH26 pKa = 8.5KK27 pKa = 10.47SVQIIHH33 pKa = 7.63PDD35 pKa = 3.2LEE37 pKa = 5.37IYY39 pKa = 9.54LKK41 pKa = 10.01KK42 pKa = 8.96WARR45 pKa = 11.84VRR47 pKa = 11.84QLSFLQDD54 pKa = 3.56LLMCKK59 pKa = 10.13GLANPVPDD67 pKa = 3.95EE68 pKa = 5.12MILQSYY74 pKa = 9.32IDD76 pKa = 3.68HH77 pKa = 6.75RR78 pKa = 11.84QTLSSVGEE86 pKa = 4.18SPEE89 pKa = 3.9GFLMEE94 pKa = 4.64FKK96 pKa = 10.64KK97 pKa = 10.82VLRR100 pKa = 11.84TFGEE104 pKa = 4.36GLRR107 pKa = 11.84KK108 pKa = 10.15NFDD111 pKa = 3.32GKK113 pKa = 8.53TVYY116 pKa = 10.21PSEE119 pKa = 4.0TGYY122 pKa = 11.42LGFTRR127 pKa = 11.84SKK129 pKa = 10.96GGLRR133 pKa = 11.84TALKK137 pKa = 10.45GQFRR141 pKa = 11.84PKK143 pKa = 10.49GFRR146 pKa = 11.84KK147 pKa = 9.72RR148 pKa = 11.84LGRR151 pKa = 11.84IDD153 pKa = 5.13PIVLHH158 pKa = 6.6LEE160 pKa = 4.02GLPGIGKK167 pKa = 9.86SKK169 pKa = 10.57ISLEE173 pKa = 3.84LGKK176 pKa = 10.46RR177 pKa = 11.84ISKK180 pKa = 10.69AFGFNYY186 pKa = 9.77NDD188 pKa = 3.41MYY190 pKa = 11.52NRR192 pKa = 11.84TINCDD197 pKa = 2.43HH198 pKa = 7.02WDD200 pKa = 3.8GYY202 pKa = 10.65RR203 pKa = 11.84GQLISSIDD211 pKa = 3.54DD212 pKa = 4.03FGCSFEE218 pKa = 4.67RR219 pKa = 11.84QSSDD223 pKa = 3.28YY224 pKa = 11.17GSLIQLCSEE233 pKa = 4.2QEE235 pKa = 4.21MVLPMADD242 pKa = 3.59LKK244 pKa = 11.32EE245 pKa = 4.3KK246 pKa = 10.37GRR248 pKa = 11.84KK249 pKa = 8.74FSSEE253 pKa = 3.95FLFLSTNGLTTCPTYY268 pKa = 10.78NIHH271 pKa = 5.85TVNTFGSALLRR282 pKa = 11.84RR283 pKa = 11.84VSPTFKK289 pKa = 10.8LEE291 pKa = 3.85RR292 pKa = 11.84GPNYY296 pKa = 10.12SYY298 pKa = 11.76RR299 pKa = 11.84MLKK302 pKa = 10.07KK303 pKa = 9.01EE304 pKa = 3.84YY305 pKa = 10.31SVEE308 pKa = 4.06TEE310 pKa = 3.19RR311 pKa = 11.84WNQVGYY317 pKa = 9.99WEE319 pKa = 4.81GDD321 pKa = 3.11WLKK324 pKa = 10.0MVSILEE330 pKa = 4.12SDD332 pKa = 3.55LLSRR336 pKa = 11.84FDD338 pKa = 4.03KK339 pKa = 11.1KK340 pKa = 10.67FGNIFQPCTSSSFGEE355 pKa = 4.15VGYY358 pKa = 10.99GFYY361 pKa = 10.63FPSNPPDD368 pKa = 3.65RR369 pKa = 11.84LPEE372 pKa = 4.32CEE374 pKa = 3.71AHH376 pKa = 7.65AIAEE380 pKa = 4.14PLKK383 pKa = 10.99VRR385 pKa = 11.84LITKK389 pKa = 9.66NEE391 pKa = 3.85PNTWVLKK398 pKa = 9.72PVQLAMWKK406 pKa = 7.38TLKK409 pKa = 10.37NFKK412 pKa = 10.31VFSLTHH418 pKa = 6.44GPTIDD423 pKa = 4.38LSRR426 pKa = 11.84LGKK429 pKa = 9.8GGRR432 pKa = 11.84YY433 pKa = 8.92LVSGDD438 pKa = 3.67YY439 pKa = 10.73KK440 pKa = 10.85SATDD444 pKa = 3.8RR445 pKa = 11.84LHH447 pKa = 6.79FDD449 pKa = 3.19IMQTAVNVLKK459 pKa = 10.57DD460 pKa = 4.09YY461 pKa = 10.87IPEE464 pKa = 4.0EE465 pKa = 3.94LRR467 pKa = 11.84SWFLWEE473 pKa = 4.49GGKK476 pKa = 10.02HH477 pKa = 5.9IINYY481 pKa = 7.56PKK483 pKa = 9.43NTLLEE488 pKa = 5.02PIVQSRR494 pKa = 11.84GQLMGSLLSFPILCLANFTTYY515 pKa = 11.28AMARR519 pKa = 11.84LDD521 pKa = 4.3CFPSLEE527 pKa = 4.61IEE529 pKa = 5.78DD530 pKa = 4.18ILNDD534 pKa = 3.59EE535 pKa = 5.17NISCFINGDD544 pKa = 4.36DD545 pKa = 5.41ILFTCDD551 pKa = 3.42GKK553 pKa = 10.88NSMLYY558 pKa = 10.24NRR560 pKa = 11.84WKK562 pKa = 10.45HH563 pKa = 4.74HH564 pKa = 6.17AKK566 pKa = 10.55SIGLEE571 pKa = 3.86LSVGKK576 pKa = 8.74TYY578 pKa = 10.33ISKK581 pKa = 10.54YY582 pKa = 9.89FGLINSQMILAYY594 pKa = 9.82PKK596 pKa = 10.69VQDD599 pKa = 4.03KK600 pKa = 10.92AIKK603 pKa = 9.4KK604 pKa = 9.08LKK606 pKa = 10.67NVVQVFNNDD615 pKa = 3.01TLTISTIPCLVQLLEE630 pKa = 4.17GKK632 pKa = 9.36IRR634 pKa = 11.84SYY636 pKa = 11.69SKK638 pKa = 9.22IQNYY642 pKa = 9.9GSLDD646 pKa = 3.66FSQVLTYY653 pKa = 9.99VKK655 pKa = 10.58RR656 pKa = 11.84EE657 pKa = 4.01TIVNLNRR664 pKa = 11.84KK665 pKa = 9.24KK666 pKa = 10.69LDD668 pKa = 3.45QTPEE672 pKa = 3.99SLDD675 pKa = 3.56LPKK678 pKa = 10.55EE679 pKa = 3.86LGGIGVLKK687 pKa = 10.44EE688 pKa = 4.37GYY690 pKa = 10.12KK691 pKa = 9.07PTLKK695 pKa = 10.77DD696 pKa = 3.13KK697 pKa = 10.7EE698 pKa = 4.42VYY700 pKa = 9.37IFKK703 pKa = 10.72ALRR706 pKa = 11.84HH707 pKa = 5.75RR708 pKa = 11.84PKK710 pKa = 10.01IIQRR714 pKa = 11.84IDD716 pKa = 3.18EE717 pKa = 4.3SLLICRR723 pKa = 11.84VPMRR727 pKa = 11.84MEE729 pKa = 3.41TRR731 pKa = 11.84AYY733 pKa = 8.38STSAFHH739 pKa = 7.58ISIKK743 pKa = 9.65PYY745 pKa = 9.25HH746 pKa = 6.25GFVEE750 pKa = 4.6EE751 pKa = 4.24EE752 pKa = 4.48EE753 pKa = 4.47EE754 pKa = 4.72PEE756 pKa = 4.14FPPIFPWVEE765 pKa = 3.53FRR767 pKa = 11.84KK768 pKa = 10.08FLTWYY773 pKa = 9.27KK774 pKa = 8.54KK775 pKa = 8.52TKK777 pKa = 9.22IRR779 pKa = 11.84EE780 pKa = 4.23IINDD784 pKa = 4.07LDD786 pKa = 4.32LFNCPSLDD794 pKa = 3.39QFKK797 pKa = 10.74SYY799 pKa = 11.12LRR801 pKa = 11.84ITDD804 pKa = 3.99LKK806 pKa = 9.14TFRR809 pKa = 11.84RR810 pKa = 11.84LEE812 pKa = 4.03AFASHH817 pKa = 7.19FLCEE821 pKa = 4.1DD822 pKa = 3.18QVPNEE827 pKa = 4.13DD828 pKa = 3.24RR829 pKa = 4.37

Molecular weight: 95.7 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A1L3KI29|A0A1L3KI29_9VIRU RNA-dependent RNA polymerase OS=Beihai narna-like virus 13 OX=1922440 PE=4 SV=1

MM1 pKa = 7.55AKK3 pKa = 9.69IFKK6 pKa = 10.5LSIHH10 pKa = 5.82TFFSKK15 pKa = 10.03CTDD18 pKa = 3.09QLLPEE23 pKa = 4.52DD24 pKa = 3.75HH25 pKa = 7.08HH26 pKa = 8.5KK27 pKa = 10.47SVQIIHH33 pKa = 7.63PDD35 pKa = 3.2LEE37 pKa = 5.37IYY39 pKa = 9.54LKK41 pKa = 10.01KK42 pKa = 8.96WARR45 pKa = 11.84VRR47 pKa = 11.84QLSFLQDD54 pKa = 3.56LLMCKK59 pKa = 10.13GLANPVPDD67 pKa = 3.95EE68 pKa = 5.12MILQSYY74 pKa = 9.32IDD76 pKa = 3.68HH77 pKa = 6.75RR78 pKa = 11.84QTLSSVGEE86 pKa = 4.18SPEE89 pKa = 3.9GFLMEE94 pKa = 4.64FKK96 pKa = 10.64KK97 pKa = 10.82VLRR100 pKa = 11.84TFGEE104 pKa = 4.36GLRR107 pKa = 11.84KK108 pKa = 10.15NFDD111 pKa = 3.32GKK113 pKa = 8.53TVYY116 pKa = 10.21PSEE119 pKa = 4.0TGYY122 pKa = 11.42LGFTRR127 pKa = 11.84SKK129 pKa = 10.96GGLRR133 pKa = 11.84TALKK137 pKa = 10.45GQFRR141 pKa = 11.84PKK143 pKa = 10.49GFRR146 pKa = 11.84KK147 pKa = 9.72RR148 pKa = 11.84LGRR151 pKa = 11.84IDD153 pKa = 5.13PIVLHH158 pKa = 6.6LEE160 pKa = 4.02GLPGIGKK167 pKa = 9.86SKK169 pKa = 10.57ISLEE173 pKa = 3.84LGKK176 pKa = 10.46RR177 pKa = 11.84ISKK180 pKa = 10.69AFGFNYY186 pKa = 9.77NDD188 pKa = 3.41MYY190 pKa = 11.52NRR192 pKa = 11.84TINCDD197 pKa = 2.43HH198 pKa = 7.02WDD200 pKa = 3.8GYY202 pKa = 10.65RR203 pKa = 11.84GQLISSIDD211 pKa = 3.54DD212 pKa = 4.03FGCSFEE218 pKa = 4.67RR219 pKa = 11.84QSSDD223 pKa = 3.28YY224 pKa = 11.17GSLIQLCSEE233 pKa = 4.2QEE235 pKa = 4.21MVLPMADD242 pKa = 3.59LKK244 pKa = 11.32EE245 pKa = 4.3KK246 pKa = 10.37GRR248 pKa = 11.84KK249 pKa = 8.74FSSEE253 pKa = 3.95FLFLSTNGLTTCPTYY268 pKa = 10.78NIHH271 pKa = 5.85TVNTFGSALLRR282 pKa = 11.84RR283 pKa = 11.84VSPTFKK289 pKa = 10.8LEE291 pKa = 3.85RR292 pKa = 11.84GPNYY296 pKa = 10.12SYY298 pKa = 11.76RR299 pKa = 11.84MLKK302 pKa = 10.07KK303 pKa = 9.01EE304 pKa = 3.84YY305 pKa = 10.31SVEE308 pKa = 4.06TEE310 pKa = 3.19RR311 pKa = 11.84WNQVGYY317 pKa = 9.99WEE319 pKa = 4.81GDD321 pKa = 3.11WLKK324 pKa = 10.0MVSILEE330 pKa = 4.12SDD332 pKa = 3.55LLSRR336 pKa = 11.84FDD338 pKa = 4.03KK339 pKa = 11.1KK340 pKa = 10.67FGNIFQPCTSSSFGEE355 pKa = 4.15VGYY358 pKa = 10.99GFYY361 pKa = 10.63FPSNPPDD368 pKa = 3.65RR369 pKa = 11.84LPEE372 pKa = 4.32CEE374 pKa = 3.71AHH376 pKa = 7.65AIAEE380 pKa = 4.14PLKK383 pKa = 10.99VRR385 pKa = 11.84LITKK389 pKa = 9.66NEE391 pKa = 3.85PNTWVLKK398 pKa = 9.72PVQLAMWKK406 pKa = 7.38TLKK409 pKa = 10.37NFKK412 pKa = 10.31VFSLTHH418 pKa = 6.44GPTIDD423 pKa = 4.38LSRR426 pKa = 11.84LGKK429 pKa = 9.8GGRR432 pKa = 11.84YY433 pKa = 8.92LVSGDD438 pKa = 3.67YY439 pKa = 10.73KK440 pKa = 10.85SATDD444 pKa = 3.8RR445 pKa = 11.84LHH447 pKa = 6.79FDD449 pKa = 3.19IMQTAVNVLKK459 pKa = 10.57DD460 pKa = 4.09YY461 pKa = 10.87IPEE464 pKa = 4.0EE465 pKa = 3.94LRR467 pKa = 11.84SWFLWEE473 pKa = 4.49GGKK476 pKa = 10.02HH477 pKa = 5.9IINYY481 pKa = 7.56PKK483 pKa = 9.43NTLLEE488 pKa = 5.02PIVQSRR494 pKa = 11.84GQLMGSLLSFPILCLANFTTYY515 pKa = 11.28AMARR519 pKa = 11.84LDD521 pKa = 4.3CFPSLEE527 pKa = 4.61IEE529 pKa = 5.78DD530 pKa = 4.18ILNDD534 pKa = 3.59EE535 pKa = 5.17NISCFINGDD544 pKa = 4.36DD545 pKa = 5.41ILFTCDD551 pKa = 3.42GKK553 pKa = 10.88NSMLYY558 pKa = 10.24NRR560 pKa = 11.84WKK562 pKa = 10.45HH563 pKa = 4.74HH564 pKa = 6.17AKK566 pKa = 10.55SIGLEE571 pKa = 3.86LSVGKK576 pKa = 8.74TYY578 pKa = 10.33ISKK581 pKa = 10.54YY582 pKa = 9.89FGLINSQMILAYY594 pKa = 9.82PKK596 pKa = 10.69VQDD599 pKa = 4.03KK600 pKa = 10.92AIKK603 pKa = 9.4KK604 pKa = 9.08LKK606 pKa = 10.67NVVQVFNNDD615 pKa = 3.01TLTISTIPCLVQLLEE630 pKa = 4.17GKK632 pKa = 9.36IRR634 pKa = 11.84SYY636 pKa = 11.69SKK638 pKa = 9.22IQNYY642 pKa = 9.9GSLDD646 pKa = 3.66FSQVLTYY653 pKa = 9.99VKK655 pKa = 10.58RR656 pKa = 11.84EE657 pKa = 4.01TIVNLNRR664 pKa = 11.84KK665 pKa = 9.24KK666 pKa = 10.69LDD668 pKa = 3.45QTPEE672 pKa = 3.99SLDD675 pKa = 3.56LPKK678 pKa = 10.55EE679 pKa = 3.86LGGIGVLKK687 pKa = 10.44EE688 pKa = 4.37GYY690 pKa = 10.12KK691 pKa = 9.07PTLKK695 pKa = 10.77DD696 pKa = 3.13KK697 pKa = 10.7EE698 pKa = 4.42VYY700 pKa = 9.37IFKK703 pKa = 10.72ALRR706 pKa = 11.84HH707 pKa = 5.75RR708 pKa = 11.84PKK710 pKa = 10.01IIQRR714 pKa = 11.84IDD716 pKa = 3.18EE717 pKa = 4.3SLLICRR723 pKa = 11.84VPMRR727 pKa = 11.84MEE729 pKa = 3.41TRR731 pKa = 11.84AYY733 pKa = 8.38STSAFHH739 pKa = 7.58ISIKK743 pKa = 9.65PYY745 pKa = 9.25HH746 pKa = 6.25GFVEE750 pKa = 4.6EE751 pKa = 4.24EE752 pKa = 4.48EE753 pKa = 4.47EE754 pKa = 4.72PEE756 pKa = 4.14FPPIFPWVEE765 pKa = 3.53FRR767 pKa = 11.84KK768 pKa = 10.08FLTWYY773 pKa = 9.27KK774 pKa = 8.54KK775 pKa = 8.52TKK777 pKa = 9.22IRR779 pKa = 11.84EE780 pKa = 4.23IINDD784 pKa = 4.07LDD786 pKa = 4.32LFNCPSLDD794 pKa = 3.39QFKK797 pKa = 10.74SYY799 pKa = 11.12LRR801 pKa = 11.84ITDD804 pKa = 3.99LKK806 pKa = 9.14TFRR809 pKa = 11.84RR810 pKa = 11.84LEE812 pKa = 4.03AFASHH817 pKa = 7.19FLCEE821 pKa = 4.1DD822 pKa = 3.18QVPNEE827 pKa = 4.13DD828 pKa = 3.24RR829 pKa = 4.37

Molecular weight: 95.7 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1	0	1	829	829	829	829.0	95.7

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
2.895 ± 0.0	1.93 ± 0.0	5.187 ± 0.0	6.393 ± 0.0	6.031 ± 0.0	6.393 ± 0.0	2.171 ± 0.0	6.755 ± 0.0	8.203 ± 0.0	11.46 ± 0.0

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.051 ± 0.0	4.222 ± 0.0	4.946 ± 0.0	3.257 ± 0.0	5.549 ± 0.0	7.479 ± 0.0	5.187 ± 0.0	4.463 ± 0.0	1.448 ± 0.0	3.981 ± 0.0

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski