Deinococcus ficus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Deinococcus-Thermus; Deinococci; Deinococcales;

Average proteome isoelectric point is 6.77

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3884 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A221SW75|A0A221SW75_9DEIO Uncharacterized protein OS=Deinococcus ficus OX=317577 GN=DFI_07720 PE=4 SV=1
MM1 pKa = 7.62SEE3 pKa = 3.55PAAAVILLYY12 pKa = 9.76PPPGHH17 pKa = 6.31EE18 pKa = 4.26VDD20 pKa = 3.8PQALHH25 pKa = 7.22DD26 pKa = 4.85LEE28 pKa = 4.29THH30 pKa = 6.24AAEE33 pKa = 4.61TYY35 pKa = 9.73DD36 pKa = 3.55AGVILSPGQMLSGGGATLLIGQWFPYY62 pKa = 10.26HH63 pKa = 7.01PDD65 pKa = 3.03EE66 pKa = 5.32VMEE69 pKa = 5.72DD70 pKa = 3.4LARR73 pKa = 11.84WIDD76 pKa = 3.4RR77 pKa = 11.84VFFNLDD83 pKa = 2.84WLQLDD88 pKa = 4.09EE89 pKa = 4.56VSS91 pKa = 3.98

Molecular weight: 10.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A221T3N9|A0A221T3N9_9DEIO Beta sliding clamp OS=Deinococcus ficus OX=317577 GN=DFI_19395 PE=3 SV=1
MM1 pKa = 6.94RR2 pKa = 11.84TGWLRR7 pKa = 11.84GPRR10 pKa = 11.84PRR12 pKa = 11.84GLQVRR17 pKa = 11.84PEE19 pKa = 4.18ALPLCARR26 pKa = 11.84LVEE29 pKa = 4.39RR30 pKa = 11.84RR31 pKa = 11.84AARR34 pKa = 11.84PPRR37 pKa = 11.84ASFQGDD43 pKa = 3.62GSSGRR48 pKa = 11.84AGRR51 pKa = 11.84PRR53 pKa = 11.84PGSPSPARR61 pKa = 11.84RR62 pKa = 11.84SVPGGGAARR71 pKa = 11.84AWFRR75 pKa = 11.84PGRR78 pKa = 11.84RR79 pKa = 11.84TPPGRR84 pKa = 11.84AGAARR89 pKa = 11.84PHH91 pKa = 6.21GAGVRR96 pKa = 11.84HH97 pKa = 5.53TPRR100 pKa = 11.84RR101 pKa = 11.84GPRR104 pKa = 11.84TGRR107 pKa = 11.84RR108 pKa = 11.84HH109 pKa = 6.58SSHH112 pKa = 6.01LWGRR116 pKa = 11.84SGPLHH121 pKa = 6.77RR122 pKa = 11.84PGGG125 pKa = 3.75

Molecular weight: 13.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3884

0

3884

1209110

26

3292

311.3

33.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.791 ± 0.054

0.587 ± 0.01

5.368 ± 0.029

5.156 ± 0.038

3.061 ± 0.024

9.061 ± 0.045

2.373 ± 0.025

3.268 ± 0.034

2.331 ± 0.04

11.673 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.933 ± 0.019

2.339 ± 0.025

6.091 ± 0.036

3.852 ± 0.027

7.496 ± 0.048

4.628 ± 0.027

6.42 ± 0.044

7.937 ± 0.035

1.372 ± 0.017

2.264 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski