Mycobacterium phage Galactic

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Cheoctovirus; unclassified Cheoctovirus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A385UCS5|A0A385UCS5_9CAUD Holin OS=Mycobacterium phage Galactic OX=2301612 GN=34 PE=4 SV=1
MM1 pKa = 7.54SSYY4 pKa = 10.66KK5 pKa = 10.06IRR7 pKa = 11.84VEE9 pKa = 3.92AKK11 pKa = 9.06AVEE14 pKa = 4.8FYY16 pKa = 10.72EE17 pKa = 4.84IEE19 pKa = 4.42AEE21 pKa = 4.25SEE23 pKa = 3.53DD24 pKa = 3.83DD25 pKa = 5.59ARR27 pKa = 11.84DD28 pKa = 3.3RR29 pKa = 11.84WHH31 pKa = 6.8EE32 pKa = 4.06GEE34 pKa = 4.26FLHH37 pKa = 8.05SEE39 pKa = 4.29VYY41 pKa = 9.99DD42 pKa = 4.02AYY44 pKa = 11.35VDD46 pKa = 3.79TVEE49 pKa = 6.04LIDD52 pKa = 3.74EE53 pKa = 4.39

Molecular weight: 6.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A385UEF5|A0A385UEF5_9CAUD Minor tail protein OS=Mycobacterium phage Galactic OX=2301612 GN=16 PE=4 SV=1
MM1 pKa = 7.51SSATSRR7 pKa = 11.84IDD9 pKa = 3.01KK10 pKa = 8.81TGPGARR16 pKa = 11.84QRR18 pKa = 11.84IPTPNPITGLSRR30 pKa = 11.84EE31 pKa = 4.16GLAVDD36 pKa = 3.87ATHH39 pKa = 6.59EE40 pKa = 4.04EE41 pKa = 3.91WRR43 pKa = 11.84PVVGFEE49 pKa = 4.46GLYY52 pKa = 10.11EE53 pKa = 3.99VSNLGRR59 pKa = 11.84VRR61 pKa = 11.84SLDD64 pKa = 2.98HH65 pKa = 6.46FARR68 pKa = 11.84GRR70 pKa = 11.84SGSKK74 pKa = 9.99RR75 pKa = 11.84LIHH78 pKa = 6.39GRR80 pKa = 11.84VLRR83 pKa = 11.84PAPRR87 pKa = 11.84TSGHH91 pKa = 5.84LTVALGRR98 pKa = 11.84NGGSKK103 pKa = 10.31DD104 pKa = 3.35VHH106 pKa = 5.79TLVATAFIGPRR117 pKa = 11.84PEE119 pKa = 4.96GMEE122 pKa = 4.52CCHH125 pKa = 6.98QDD127 pKa = 3.81GDD129 pKa = 4.02PTNNRR134 pKa = 11.84VEE136 pKa = 4.07NLRR139 pKa = 11.84WDD141 pKa = 3.57TRR143 pKa = 11.84SANRR147 pKa = 11.84LDD149 pKa = 3.89SVRR152 pKa = 11.84HH153 pKa = 4.99GTHH156 pKa = 4.89QAVKK160 pKa = 9.03KK161 pKa = 6.01THH163 pKa = 6.55CKK165 pKa = 9.72HH166 pKa = 5.21GHH168 pKa = 5.66EE169 pKa = 4.33FTPEE173 pKa = 3.46NTLVQRR179 pKa = 11.84GKK181 pKa = 9.87HH182 pKa = 4.27RR183 pKa = 11.84RR184 pKa = 11.84CRR186 pKa = 11.84EE187 pKa = 3.7CHH189 pKa = 6.58RR190 pKa = 11.84LDD192 pKa = 3.54SQKK195 pKa = 11.0GYY197 pKa = 9.93PRR199 pKa = 4.67

Molecular weight: 22.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

109

0

109

18556

30

1175

170.2

18.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.239 ± 0.427

1.229 ± 0.181

6.661 ± 0.218

5.858 ± 0.307

3.093 ± 0.192

8.897 ± 0.537

2.387 ± 0.221

4.338 ± 0.177

3.379 ± 0.181

7.405 ± 0.254

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.177 ± 0.121

3.395 ± 0.143

6.003 ± 0.224

3.427 ± 0.187

6.936 ± 0.399

5.75 ± 0.317

6.806 ± 0.238

7.194 ± 0.243

2.242 ± 0.146

2.581 ± 0.165
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski