Heterobasidion partitivirus 15

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Duplopiviricetes; Durnavirales; Partitiviridae; Alphapartitivirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W8PR84|W8PR84_9VIRU RNA-dependent RNA polymerase OS=Heterobasidion partitivirus 15 OX=1469908 PE=4 SV=1
MM1 pKa = 7.55SATKK5 pKa = 10.05MEE7 pKa = 5.36DD8 pKa = 3.52YY9 pKa = 11.49LNGKK13 pKa = 9.09LDD15 pKa = 3.62AAALNKK21 pKa = 10.2ADD23 pKa = 3.46IEE25 pKa = 4.42AYY27 pKa = 10.09AAAQIKK33 pKa = 8.77AQKK36 pKa = 10.45DD37 pKa = 3.56LIEE40 pKa = 4.94ASTSASKK47 pKa = 10.87DD48 pKa = 3.25AGSDD52 pKa = 3.5PLGMRR57 pKa = 11.84DD58 pKa = 3.39VHH60 pKa = 6.88KK61 pKa = 10.43QAQDD65 pKa = 3.22SVQAQSQPTSTNLRR79 pKa = 11.84PPRR82 pKa = 11.84FQKK85 pKa = 10.46VDD87 pKa = 3.3DD88 pKa = 3.84TDD90 pKa = 5.0AINAHH95 pKa = 6.58RR96 pKa = 11.84LVLMDD101 pKa = 5.5DD102 pKa = 4.28PFTHH106 pKa = 6.39VSKK109 pKa = 10.8FGNNFVVPDD118 pKa = 4.07FHH120 pKa = 7.85HH121 pKa = 7.11AFTMLDD127 pKa = 3.27EE128 pKa = 4.91MDD130 pKa = 5.24NIMLSTKK137 pKa = 10.15KK138 pKa = 9.39FTDD141 pKa = 3.45ISEE144 pKa = 4.1TWTPLMSRR152 pKa = 11.84YY153 pKa = 9.61FFGSLLLVHH162 pKa = 6.43TLRR165 pKa = 11.84VQRR168 pKa = 11.84EE169 pKa = 3.9ADD171 pKa = 3.24AVTPTVANFLDD182 pKa = 3.88QFEE185 pKa = 5.02KK186 pKa = 10.89DD187 pKa = 4.12FPLHH191 pKa = 6.64SIPIPGPMVNFFEE204 pKa = 5.07CLAASNVAVAGFDD217 pKa = 3.53PVSPILPKK225 pKa = 10.19KK226 pKa = 10.75ANVTTRR232 pKa = 11.84AKK234 pKa = 10.92LLFNNNLAGRR244 pKa = 11.84IPHH247 pKa = 6.54PLIMLDD253 pKa = 3.54QMHH256 pKa = 6.13VLINAMGNGDD266 pKa = 3.79RR267 pKa = 11.84ATRR270 pKa = 11.84ISNVANCSVFFNGIFSIGMSNAASADD296 pKa = 3.85PYY298 pKa = 10.74EE299 pKa = 4.34QTPVHH304 pKa = 6.27AVNTANHH311 pKa = 5.99IAWVFSYY318 pKa = 10.76PDD320 pKa = 2.9VAYY323 pKa = 9.29PVLSSYY329 pKa = 10.77NAASSFVDD337 pKa = 4.15NINQLRR343 pKa = 11.84RR344 pKa = 11.84VLPARR349 pKa = 11.84LDD351 pKa = 3.45LSGAGNATQLEE362 pKa = 4.53WPEE365 pKa = 3.94FVSIMEE371 pKa = 3.97YY372 pKa = 10.19PRR374 pKa = 11.84LFRR377 pKa = 11.84YY378 pKa = 9.6ILRR381 pKa = 11.84LMAEE385 pKa = 4.11YY386 pKa = 11.02SKK388 pKa = 10.57FWKK391 pKa = 10.32GSKK394 pKa = 10.27SLDD397 pKa = 4.14LIPLAGKK404 pKa = 9.66PSLQVAFTKK413 pKa = 10.7DD414 pKa = 3.69GAEE417 pKa = 3.81HH418 pKa = 7.34GYY420 pKa = 7.58PTTRR424 pKa = 11.84FPTFTHH430 pKa = 6.5SFTGAVRR437 pKa = 11.84RR438 pKa = 11.84SQISPADD445 pKa = 4.69RR446 pKa = 11.84IDD448 pKa = 3.62AATSVLNYY456 pKa = 10.58SHH458 pKa = 7.18LADD461 pKa = 4.17GGGIDD466 pKa = 4.28RR467 pKa = 11.84ANPGIPRR474 pKa = 11.84QGGPFFDD481 pKa = 5.0DD482 pKa = 3.62SEE484 pKa = 4.5IVLRR488 pKa = 11.84TRR490 pKa = 11.84NINPSLGYY498 pKa = 10.73GSVATEE504 pKa = 4.38KK505 pKa = 10.95YY506 pKa = 9.91HH507 pKa = 7.22LKK509 pKa = 8.2NTKK512 pKa = 9.61

Molecular weight: 56.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W8PR84|W8PR84_9VIRU RNA-dependent RNA polymerase OS=Heterobasidion partitivirus 15 OX=1469908 PE=4 SV=1
MM1 pKa = 7.53FSLLTWLSTKK11 pKa = 10.18FNALFDD17 pKa = 4.19SVYY20 pKa = 9.57STRR23 pKa = 11.84FEE25 pKa = 4.79HH26 pKa = 6.93NFRR29 pKa = 11.84FLYY32 pKa = 10.05KK33 pKa = 10.18AAPLTTPHH41 pKa = 7.19RR42 pKa = 11.84DD43 pKa = 2.99EE44 pKa = 4.19TKK46 pKa = 8.62YY47 pKa = 11.03AEE49 pKa = 3.93YY50 pKa = 10.0QAYY53 pKa = 9.82VKK55 pKa = 9.91STLDD59 pKa = 3.44HH60 pKa = 6.4VLLGPDD66 pKa = 2.8AHH68 pKa = 6.9HH69 pKa = 6.76IVNGYY74 pKa = 7.96HH75 pKa = 6.58HH76 pKa = 7.84PIATLDD82 pKa = 3.51AVINTFKK89 pKa = 11.0KK90 pKa = 10.79GDD92 pKa = 3.96LPDD95 pKa = 3.67HH96 pKa = 7.09KK97 pKa = 10.43IPKK100 pKa = 8.97DD101 pKa = 3.27LHH103 pKa = 6.49YY104 pKa = 11.16YY105 pKa = 9.9SALLEE110 pKa = 4.3TTKK113 pKa = 10.69RR114 pKa = 11.84FAPPQKK120 pKa = 10.07LRR122 pKa = 11.84PVHH125 pKa = 6.3FADD128 pKa = 4.35LRR130 pKa = 11.84FYY132 pKa = 10.37KK133 pKa = 10.12WNWHH137 pKa = 6.26PNVEE141 pKa = 4.17EE142 pKa = 4.25PFRR145 pKa = 11.84SEE147 pKa = 5.57AEE149 pKa = 3.86LQQAVEE155 pKa = 4.24AAYY158 pKa = 9.87HH159 pKa = 6.64AGLLPDD165 pKa = 3.5GRR167 pKa = 11.84MSFGNLKK174 pKa = 9.49NVVFIRR180 pKa = 11.84VRR182 pKa = 11.84EE183 pKa = 3.89WLHH186 pKa = 4.79QIKK189 pKa = 10.09RR190 pKa = 11.84AQITNPNTLYY200 pKa = 10.29PLINIHH206 pKa = 6.06VKK208 pKa = 9.89PALTTPDD215 pKa = 3.12EE216 pKa = 4.25TKK218 pKa = 10.01IRR220 pKa = 11.84VVYY223 pKa = 10.01GVSKK227 pKa = 10.35LHH229 pKa = 5.83VLPEE233 pKa = 4.17AMFFWPLFRR242 pKa = 11.84HH243 pKa = 5.55YY244 pKa = 10.99LDD246 pKa = 3.84NRR248 pKa = 11.84KK249 pKa = 9.37EE250 pKa = 4.43SPLLWGFEE258 pKa = 4.33TILGGMAVLHH268 pKa = 6.28NMMTIPRR275 pKa = 11.84LYY277 pKa = 8.89FQTFVMVDD285 pKa = 2.48WSGFDD290 pKa = 3.43LRR292 pKa = 11.84SLFEE296 pKa = 4.72PIRR299 pKa = 11.84TDD301 pKa = 3.62VFPAWRR307 pKa = 11.84TYY309 pKa = 10.68FDD311 pKa = 4.42FNNGYY316 pKa = 9.64IPTRR320 pKa = 11.84FYY322 pKa = 10.21RR323 pKa = 11.84TSKK326 pKa = 10.95ADD328 pKa = 3.52PEE330 pKa = 4.21HH331 pKa = 7.24LKK333 pKa = 10.74RR334 pKa = 11.84LWEE337 pKa = 4.07WTRR340 pKa = 11.84EE341 pKa = 3.81ACLRR345 pKa = 11.84MPHH348 pKa = 6.96RR349 pKa = 11.84MMDD352 pKa = 3.44GSVYY356 pKa = 10.2EE357 pKa = 4.08RR358 pKa = 11.84LFRR361 pKa = 11.84CIPSGLFTTQFLDD374 pKa = 3.11SFYY377 pKa = 11.35NMLMILTILSAMGIDD392 pKa = 3.19ISTVVIRR399 pKa = 11.84VQGDD403 pKa = 3.17DD404 pKa = 4.05SLIMLQFFLPANQHH418 pKa = 5.45EE419 pKa = 4.61EE420 pKa = 4.1FKK422 pKa = 11.38ARR424 pKa = 11.84FQALATYY431 pKa = 9.85YY432 pKa = 9.6FDD434 pKa = 5.8HH435 pKa = 7.31IARR438 pKa = 11.84PEE440 pKa = 3.87KK441 pKa = 10.16TDD443 pKa = 3.06ISNSPEE449 pKa = 3.75GVNVLGYY456 pKa = 8.94TNEE459 pKa = 3.78NGYY462 pKa = 9.13PIRR465 pKa = 11.84DD466 pKa = 3.36WRR468 pKa = 11.84KK469 pKa = 9.16LLAQLYY475 pKa = 9.05HH476 pKa = 6.8PRR478 pKa = 11.84SQRR481 pKa = 11.84PTLEE485 pKa = 3.79LLKK488 pKa = 11.02ARR490 pKa = 11.84VCGIQYY496 pKa = 10.88ASMYY500 pKa = 9.76RR501 pKa = 11.84YY502 pKa = 8.52PQVTEE507 pKa = 4.07VCQTLWNRR515 pKa = 11.84LEE517 pKa = 4.09ADD519 pKa = 4.2GIKK522 pKa = 10.52ALNLAAQRR530 pKa = 11.84DD531 pKa = 4.64VVLHH535 pKa = 5.25SHH537 pKa = 7.15ADD539 pKa = 3.45FFIPTDD545 pKa = 3.52HH546 pKa = 7.14FPTLNEE552 pKa = 3.63VTKK555 pKa = 9.72WLRR558 pKa = 11.84VPYY561 pKa = 10.32QRR563 pKa = 11.84TAEE566 pKa = 3.95DD567 pKa = 3.53RR568 pKa = 11.84EE569 pKa = 4.48EE570 pKa = 4.19YY571 pKa = 10.62FPLSHH576 pKa = 6.92FLSYY580 pKa = 10.54FF581 pKa = 3.3

Molecular weight: 68.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1093

512

581

546.5

62.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.417 ± 1.496

0.549 ± 0.111

5.947 ± 0.625

4.3 ± 0.826

6.404 ± 0.383

4.392 ± 0.62

3.751 ± 0.577

4.849 ± 0.161

4.392 ± 0.071

9.79 ± 0.977

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.745 ± 0.13

5.032 ± 0.719

6.404 ± 0.029

3.294 ± 0.019

5.947 ± 0.748

6.038 ± 1.247

6.221 ± 0.254

5.947 ± 0.213

1.555 ± 0.544

4.026 ± 0.907
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski