Hyphomicrobium denitrificans (strain ATCC 51888 / DSM 1869 / NCIMB 11706 / TK 0415)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Hyphomicrobiaceae; Hyphomicrobium; Hyphomicrobium denitrificans

Average proteome isoelectric point is 6.72

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D8JRQ7|D8JRQ7_HYPDA Cytidylate kinase OS=Hyphomicrobium denitrificans (strain ATCC 51888 / DSM 1869 / NCIMB 11706 / TK 0415) OX=582899 GN=cmk PE=3 SV=1
MM1 pKa = 7.57TKK3 pKa = 7.94TTTAILGLLGSFACSSSAFAQAAQQEE29 pKa = 4.56IQISATVPKK38 pKa = 10.28SCTINGTSSGVDD50 pKa = 3.03TATIGIDD57 pKa = 3.1AAGDD61 pKa = 3.76VIVAPVTPTNAPYY74 pKa = 11.15LNVVCNTPSTLQLRR88 pKa = 11.84SDD90 pKa = 3.37QGAVKK95 pKa = 9.74TGATASGFASIIDD108 pKa = 3.99YY109 pKa = 10.03QASATWNGQTATLDD123 pKa = 3.4TATIATATGQEE134 pKa = 4.13TGTAEE139 pKa = 3.89PVAAGSGQLDD149 pKa = 3.53VTITPEE155 pKa = 4.37ANVQPLLGGNYY166 pKa = 9.5SDD168 pKa = 4.42SLFVLLTPQQ177 pKa = 3.37

Molecular weight: 17.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D8JSY7|D8JSY7_HYPDA Uncharacterized protein OS=Hyphomicrobium denitrificans (strain ATCC 51888 / DSM 1869 / NCIMB 11706 / TK 0415) OX=582899 GN=Hden_0651 PE=4 SV=1
MM1 pKa = 7.59SKK3 pKa = 9.5TSLKK7 pKa = 10.62NIQKK11 pKa = 9.49QKK13 pKa = 10.44KK14 pKa = 8.03KK15 pKa = 10.62ASRR18 pKa = 11.84TIPRR22 pKa = 11.84PAQSRR27 pKa = 11.84IQGNTAGVRR36 pKa = 11.84TRR38 pKa = 11.84LKK40 pKa = 10.58KK41 pKa = 10.49LAGKK45 pKa = 9.92ARR47 pKa = 11.84AAKK50 pKa = 10.35ASAA53 pKa = 3.42

Molecular weight: 5.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3452

0

3452

1051318

30

2116

304.6

33.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.254 ± 0.057

0.847 ± 0.012

5.795 ± 0.034

5.58 ± 0.042

3.689 ± 0.028

8.097 ± 0.043

1.952 ± 0.019

5.457 ± 0.029

4.204 ± 0.039

9.596 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.258 ± 0.018

2.896 ± 0.023

5.135 ± 0.032

3.128 ± 0.023

6.793 ± 0.039

6.063 ± 0.03

5.439 ± 0.031

7.222 ± 0.035

1.31 ± 0.017

2.284 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski