Southern cowpea mosaic virus (SCPMV) (Southern bean mosaic virus (strain cowpea))

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Sobelivirales; Solemoviridae; Sobemovirus

Average proteome isoelectric point is 7.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q83470|P2A_SCPMV Polyprotein P2A OS=Southern cowpea mosaic virus OX=196398 GN=ORF2A PE=4 SV=2
MM1 pKa = 8.13IYY3 pKa = 8.18EE4 pKa = 4.29TLVLSKK10 pKa = 10.13TEE12 pKa = 4.04LEE14 pKa = 4.21QLNVDD19 pKa = 4.31LPRR22 pKa = 11.84YY23 pKa = 9.16SYY25 pKa = 10.79RR26 pKa = 11.84FRR28 pKa = 11.84YY29 pKa = 9.09SRR31 pKa = 11.84SIGDD35 pKa = 3.66TVVEE39 pKa = 4.65FPGTLSDD46 pKa = 3.42PCIPVVDD53 pKa = 4.28VLLGACWAWPQPSRR67 pKa = 11.84HH68 pKa = 6.08GGLGLDD74 pKa = 5.42DD75 pKa = 5.15IDD77 pKa = 4.94PFDD80 pKa = 5.06ASFSCCVTSPEE91 pKa = 4.47RR92 pKa = 11.84YY93 pKa = 8.55CLSRR97 pKa = 11.84SVLSGVDD104 pKa = 3.22AFVVRR109 pKa = 11.84GSCKK113 pKa = 10.06LCGLGFLDD121 pKa = 3.99NFNPFEE127 pKa = 4.03IRR129 pKa = 11.84ALLGQTTPGLWWQPVKK145 pKa = 10.34PVYY148 pKa = 10.21DD149 pKa = 4.02DD150 pKa = 4.79RR151 pKa = 11.84NIHH154 pKa = 6.46LPYY157 pKa = 10.51DD158 pKa = 3.58SEE160 pKa = 4.49LNARR164 pKa = 11.84IQRR167 pKa = 11.84FQYY170 pKa = 8.84TCRR173 pKa = 11.84EE174 pKa = 4.28CIVRR178 pKa = 11.84VAFHH182 pKa = 6.23MSSS185 pKa = 2.96

Molecular weight: 20.98 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P21405|RDRP_SCPMV Replicase polyprotein P2AB OS=Southern cowpea mosaic virus OX=196398 GN=ORF2A-2B PE=3 SV=2
MM1 pKa = 7.55SGLFHH6 pKa = 7.07HH7 pKa = 6.26RR8 pKa = 11.84TKK10 pKa = 10.49PRR12 pKa = 11.84EE13 pKa = 3.39IRR15 pKa = 11.84AFVMATRR22 pKa = 11.84LTKK25 pKa = 10.36KK26 pKa = 10.17QLAQAIQNTLPNPPRR41 pKa = 11.84RR42 pKa = 11.84KK43 pKa = 9.39RR44 pKa = 11.84RR45 pKa = 11.84AKK47 pKa = 9.92RR48 pKa = 11.84RR49 pKa = 11.84AAQVPKK55 pKa = 8.55PTQAGVSMAPIAQGTMVKK73 pKa = 10.22LRR75 pKa = 11.84PPMLRR80 pKa = 11.84SSMDD84 pKa = 3.13VTILSHH90 pKa = 7.0CEE92 pKa = 3.6LSTEE96 pKa = 4.11LAVTVTIVVTSEE108 pKa = 3.58LVMPFTVGTWLRR120 pKa = 11.84GVAQNWSKK128 pKa = 10.44YY129 pKa = 7.72AWVAIRR135 pKa = 11.84YY136 pKa = 6.64TYY138 pKa = 10.79LPSCPTTTSGAIHH151 pKa = 6.6MGFQYY156 pKa = 11.58DD157 pKa = 3.61MADD160 pKa = 3.6TLPVSVNQLSNLKK173 pKa = 10.37GYY175 pKa = 7.99VTGPVWEE182 pKa = 4.78GQSGLCFVNNTKK194 pKa = 10.71CPDD197 pKa = 3.1TSRR200 pKa = 11.84AITIALDD207 pKa = 3.54TNEE210 pKa = 4.22VSEE213 pKa = 4.22KK214 pKa = 10.3RR215 pKa = 11.84YY216 pKa = 9.24PFKK219 pKa = 10.48TATDD223 pKa = 3.61YY224 pKa = 10.01ATAVGVNANIGNILVPARR242 pKa = 11.84LVTAMEE248 pKa = 4.81GGSSKK253 pKa = 9.71TAVNTGRR260 pKa = 11.84LYY262 pKa = 10.88ASYY265 pKa = 9.38TIRR268 pKa = 11.84LIEE271 pKa = 4.83PIAAALNLL279 pKa = 3.8

Molecular weight: 30.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1992

185

956

498.0

54.64

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.082 ± 0.762

2.41 ± 0.418

4.267 ± 0.474

5.221 ± 0.706

3.363 ± 0.393

7.279 ± 0.471

2.058 ± 0.216

4.016 ± 0.331

4.97 ± 0.662

9.036 ± 0.306

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.56 ± 0.289

3.313 ± 0.342

6.978 ± 0.63

3.213 ± 0.096

5.321 ± 0.656

8.735 ± 0.592

6.074 ± 1.078

8.133 ± 0.376

2.008 ± 0.156

2.962 ± 0.293
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski