Bifidobacterium scardovii

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2477 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087D7L0|A0A087D7L0_9BIFI Oxidoreductase domain-containing protein OS=Bifidobacterium scardovii OX=158787 GN=BSCA_2207 PE=4 SV=1
MM1 pKa = 7.45TNDD4 pKa = 3.1NTNPDD9 pKa = 4.15TVPGTSATDD18 pKa = 3.21SGQPSTPDD26 pKa = 3.02TAQRR30 pKa = 11.84DD31 pKa = 3.63PDD33 pKa = 3.65NAQTATVSGTDD44 pKa = 3.28NGSAGNGAPTDD55 pKa = 3.98AGSSSGTGAGNAQDD69 pKa = 3.61SAAPGGANRR78 pKa = 11.84PAWDD82 pKa = 3.49WQRR85 pKa = 11.84ADD87 pKa = 4.66SPDD90 pKa = 3.33SDD92 pKa = 3.83TRR94 pKa = 11.84QDD96 pKa = 4.09TIPQSAAGSDD106 pKa = 3.48APTTGSQPGQRR117 pKa = 11.84PAQTTGQPGYY127 pKa = 10.12AAQPGFQGYY136 pKa = 7.68GQPGYY141 pKa = 9.9GQPNGVTQPGYY152 pKa = 11.03APGYY156 pKa = 7.18MAGYY160 pKa = 9.12AAQPNYY166 pKa = 10.49SGPQAYY172 pKa = 8.84GAQPAWPQPAPNAGASGQTGTLGRR196 pKa = 11.84IGAQSLGQLTKK207 pKa = 9.64KK208 pKa = 8.21TWAILVGVALVCGLVGGAIGAAGFSALDD236 pKa = 3.34GDD238 pKa = 3.64SRR240 pKa = 11.84YY241 pKa = 9.72RR242 pKa = 11.84VQQMQMPGGMGEE254 pKa = 4.38SNGNGMGEE262 pKa = 4.08PPSGNGQSGNSGSNGSADD280 pKa = 3.47SGTGSSSYY288 pKa = 11.37SDD290 pKa = 3.92GSTSDD295 pKa = 4.52LIEE298 pKa = 4.08SS299 pKa = 3.81

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087D5C3|A0A087D5C3_9BIFI Transcriptional regulator OS=Bifidobacterium scardovii OX=158787 GN=BSCA_1576 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.67HH16 pKa = 5.74GFRR19 pKa = 11.84LRR21 pKa = 11.84LRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.06GRR39 pKa = 11.84KK40 pKa = 8.87SLSAA44 pKa = 3.86

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2477

0

2477

882320

27

3168

356.2

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.612 ± 0.063

0.931 ± 0.016

6.714 ± 0.046

5.318 ± 0.04

3.45 ± 0.03

8.524 ± 0.041

2.063 ± 0.026

5.188 ± 0.038

3.494 ± 0.043

8.809 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.643 ± 0.023

3.1 ± 0.031

4.8 ± 0.036

3.169 ± 0.03

6.289 ± 0.053

5.994 ± 0.04

5.927 ± 0.041

7.691 ± 0.042

1.451 ± 0.02

2.83 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski