Vibrio phage Rostov 6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Enhodamvirus; unclassified Enhodamvirus

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2S1GN22|A0A2S1GN22_9CAUD Integrase OS=Vibrio phage Rostov 6 OX=2116688 GN=Rostov6_00065 PE=4 SV=1
MM1 pKa = 7.74TIQNKK6 pKa = 9.59EE7 pKa = 3.95PFVYY11 pKa = 9.36ATGNGVTATIPFTFPANSASDD32 pKa = 3.62VYY34 pKa = 11.28GAINGIDD41 pKa = 3.33QSVTVVLSPDD51 pKa = 3.66QINSPGGTLTFNGGAAPLGSSVLVYY76 pKa = 10.67RR77 pKa = 11.84NTKK80 pKa = 9.0MEE82 pKa = 3.97QTLSYY87 pKa = 7.41PTKK90 pKa = 10.47GSFPSKK96 pKa = 9.01SHH98 pKa = 6.1EE99 pKa = 4.11SAMDD103 pKa = 3.69KK104 pKa = 10.94LVMIAQDD111 pKa = 4.36LNFLLASRR119 pKa = 11.84CLRR122 pKa = 11.84APNGEE127 pKa = 4.13KK128 pKa = 10.09FIKK131 pKa = 9.66EE132 pKa = 3.89LPPIQEE138 pKa = 3.93RR139 pKa = 11.84AGKK142 pKa = 9.88FLGFDD147 pKa = 3.65PAGNPYY153 pKa = 10.06AAAPIRR159 pKa = 11.84GEE161 pKa = 4.61DD162 pKa = 3.85GTDD165 pKa = 3.29GLPPYY170 pKa = 10.31ISDD173 pKa = 3.52GTDD176 pKa = 2.78GFNAGNWVAFSVQDD190 pKa = 3.59NSYY193 pKa = 10.77KK194 pKa = 9.92DD195 pKa = 3.23TGVRR199 pKa = 11.84ALGVDD204 pKa = 4.05GLPPYY209 pKa = 10.18ISNGTDD215 pKa = 2.85GFTSGNWIVDD225 pKa = 3.96DD226 pKa = 4.57GSTNYY231 pKa = 10.01VDD233 pKa = 3.07TGVRR237 pKa = 11.84AKK239 pKa = 10.66GDD241 pKa = 3.56KK242 pKa = 10.41GDD244 pKa = 4.45KK245 pKa = 10.92GDD247 pKa = 4.78DD248 pKa = 3.47ALSPYY253 pKa = 10.48VSDD256 pKa = 3.65GTDD259 pKa = 2.94GFTAGNWVADD269 pKa = 4.1NGSGAYY275 pKa = 10.11ADD277 pKa = 3.75TGIGAKK283 pKa = 9.79GDD285 pKa = 3.65KK286 pKa = 10.76GDD288 pKa = 4.2TGDD291 pKa = 5.56DD292 pKa = 3.65GLPPYY297 pKa = 10.37ISDD300 pKa = 3.9GSDD303 pKa = 2.82TFVAGNWIADD313 pKa = 3.79NGSGVYY319 pKa = 10.46ADD321 pKa = 3.55TGIPARR327 pKa = 11.84PNLQYY332 pKa = 10.42ATEE335 pKa = 4.17TTIGGMRR342 pKa = 11.84WSLVGTTLNLYY353 pKa = 8.27FTDD356 pKa = 3.53VV357 pKa = 3.13

Molecular weight: 37.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2S1GN15|A0A2S1GN15_9CAUD Peptidase_M15_3 domain-containing protein OS=Vibrio phage Rostov 6 OX=2116688 GN=Rostov6_00028 PE=4 SV=1
MM1 pKa = 7.52PRR3 pKa = 11.84KK4 pKa = 9.96CFEE7 pKa = 3.69GWKK10 pKa = 10.3EE11 pKa = 3.45EE12 pKa = 5.2DD13 pKa = 3.06IAKK16 pKa = 9.27MFGKK20 pKa = 9.09TDD22 pKa = 3.36TDD24 pKa = 4.91LITAEE29 pKa = 5.24DD30 pKa = 3.51IADD33 pKa = 4.46AIKK36 pKa = 10.59GKK38 pKa = 9.68KK39 pKa = 7.02QEE41 pKa = 4.46KK42 pKa = 8.43IAVYY46 pKa = 9.78KK47 pKa = 9.77QAEE50 pKa = 4.38AIKK53 pKa = 10.34KK54 pKa = 8.8GNEE57 pKa = 3.59VLTQSKK63 pKa = 10.83DD64 pKa = 3.3PASALLGMLSRR75 pKa = 11.84DD76 pKa = 3.37PNEE79 pKa = 3.81EE80 pKa = 3.8VRR82 pKa = 11.84FLSADD87 pKa = 3.24QRR89 pKa = 11.84INAIRR94 pKa = 11.84AVSKK98 pKa = 11.13AKK100 pKa = 10.46ISDD103 pKa = 3.84FMADD107 pKa = 4.01LAPTTRR113 pKa = 11.84QIFAGIATGEE123 pKa = 3.97RR124 pKa = 11.84RR125 pKa = 11.84LTKK128 pKa = 10.1SQQRR132 pKa = 11.84LLDD135 pKa = 4.09DD136 pKa = 4.96FVHH139 pKa = 6.27EE140 pKa = 4.37LYY142 pKa = 10.86GRR144 pKa = 11.84QTGNADD150 pKa = 3.31ALKK153 pKa = 10.39AAKK156 pKa = 9.05GWKK159 pKa = 9.5KK160 pKa = 9.59ATEE163 pKa = 4.15DD164 pKa = 3.23LNARR168 pKa = 11.84FGQAGGHH175 pKa = 4.99MAEE178 pKa = 4.55LDD180 pKa = 3.72DD181 pKa = 3.55WRR183 pKa = 11.84LPQKK187 pKa = 9.96HH188 pKa = 5.55NRR190 pKa = 11.84MAISKK195 pKa = 9.8VGADD199 pKa = 3.21VWVEE203 pKa = 4.1KK204 pKa = 10.91VWDD207 pKa = 4.76LIDD210 pKa = 4.04RR211 pKa = 11.84DD212 pKa = 3.91KK213 pKa = 10.96MVKK216 pKa = 10.02KK217 pKa = 10.2LRR219 pKa = 11.84KK220 pKa = 10.13GKK222 pKa = 10.38DD223 pKa = 3.07EE224 pKa = 4.88DD225 pKa = 3.79NLRR228 pKa = 11.84EE229 pKa = 4.07ALYY232 pKa = 10.62SVYY235 pKa = 11.17NNIVTDD241 pKa = 4.19GMSSSKK247 pKa = 9.54TLSKK251 pKa = 10.85KK252 pKa = 8.77FTDD255 pKa = 3.26MMRR258 pKa = 11.84SEE260 pKa = 4.39RR261 pKa = 11.84FITFKK266 pKa = 11.19DD267 pKa = 3.09SDD269 pKa = 3.55SWLKK273 pKa = 8.89YY274 pKa = 8.94QRR276 pKa = 11.84EE277 pKa = 4.12FGDD280 pKa = 3.78TNVYY284 pKa = 11.19ASMLGHH290 pKa = 7.29IDD292 pKa = 3.3NMSRR296 pKa = 11.84AIGMMEE302 pKa = 4.21TFGPDD307 pKa = 3.13PDD309 pKa = 3.64IGFNTLEE316 pKa = 4.17RR317 pKa = 11.84AVKK320 pKa = 8.58TKK322 pKa = 10.6KK323 pKa = 10.59GLTSRR328 pKa = 11.84QPTGARR334 pKa = 11.84PTFDD338 pKa = 2.99MLMGYY343 pKa = 10.95NMVEE347 pKa = 4.19EE348 pKa = 4.03QTVWGNRR355 pKa = 11.84VAGLRR360 pKa = 11.84NLWTASKK367 pKa = 10.51LGAAVVSALTDD378 pKa = 3.34SVYY381 pKa = 11.46ASMAASYY388 pKa = 11.13NAMSPARR395 pKa = 11.84VLRR398 pKa = 11.84RR399 pKa = 11.84MLSEE403 pKa = 3.8VMKK406 pKa = 9.81PSKK409 pKa = 10.78SEE411 pKa = 3.66ASRR414 pKa = 11.84KK415 pKa = 9.07LWAQDD420 pKa = 3.33FGFGAEE426 pKa = 4.04FALDD430 pKa = 3.77RR431 pKa = 11.84MAMTSDD437 pKa = 3.49YY438 pKa = 8.57TQSFGGHH445 pKa = 5.76RR446 pKa = 11.84SRR448 pKa = 11.84NLAEE452 pKa = 3.71AVMVVSGMNQWTQSARR468 pKa = 11.84ASFQFEE474 pKa = 4.0FATALTRR481 pKa = 11.84AADD484 pKa = 4.22SKK486 pKa = 11.01WSDD489 pKa = 3.69LPEE492 pKa = 4.25KK493 pKa = 9.71MRR495 pKa = 11.84NSMGRR500 pKa = 11.84YY501 pKa = 9.46GITEE505 pKa = 4.2SDD507 pKa = 3.06WAAIAAAPRR516 pKa = 11.84TNYY519 pKa = 9.85KK520 pKa = 8.69GNKK523 pKa = 8.82MIDD526 pKa = 3.47PRR528 pKa = 11.84NIDD531 pKa = 3.99AEE533 pKa = 4.37LQTKK537 pKa = 9.3LVGMVDD543 pKa = 4.38GEE545 pKa = 4.57TMMAVPTPDD554 pKa = 2.75ARR556 pKa = 11.84TRR558 pKa = 11.84AFMAGGTKK566 pKa = 10.05SGNFGGEE573 pKa = 3.73LHH575 pKa = 6.91RR576 pKa = 11.84SLFMFHH582 pKa = 7.09SFPITTIMNQWRR594 pKa = 11.84RR595 pKa = 11.84VFTGKK600 pKa = 10.5GYY602 pKa = 10.92NGAFDD607 pKa = 4.15RR608 pKa = 11.84MSAAAIMVGATSVLGVGIIQAKK630 pKa = 10.37DD631 pKa = 2.95ILNGKK636 pKa = 8.84KK637 pKa = 9.12PRR639 pKa = 11.84SMSDD643 pKa = 2.81PKK645 pKa = 11.07LWIEE649 pKa = 4.16GMAQGGSFNYY659 pKa = 10.07IGDD662 pKa = 4.36LMRR665 pKa = 11.84NAASGYY671 pKa = 7.93SHH673 pKa = 7.71DD674 pKa = 3.7MTSYY678 pKa = 10.6VGGPVLAYY686 pKa = 10.14GDD688 pKa = 4.21WVAMTAADD696 pKa = 3.96MAKK699 pKa = 10.46GDD701 pKa = 4.53AEE703 pKa = 4.16SAMARR708 pKa = 11.84TANFATQQIPFNNLWYY724 pKa = 9.66TKK726 pKa = 10.08IATDD730 pKa = 3.74RR731 pKa = 11.84LLMDD735 pKa = 5.18RR736 pKa = 11.84IRR738 pKa = 11.84RR739 pKa = 11.84LSDD742 pKa = 3.08PEE744 pKa = 3.89YY745 pKa = 11.05DD746 pKa = 3.37KK747 pKa = 11.59KK748 pKa = 11.03QLNKK752 pKa = 8.09MRR754 pKa = 11.84KK755 pKa = 5.49MQRR758 pKa = 11.84TSQQEE763 pKa = 4.44YY764 pKa = 7.04WWSPPIGGQSNIEE777 pKa = 4.28SPFEE781 pKa = 3.78EE782 pKa = 4.53

Molecular weight: 87.37 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15

0

15

7114

157

782

474.3

52.21

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.024 ± 0.492

0.872 ± 0.143

6.311 ± 0.421

5.707 ± 0.695

3.556 ± 0.279

9.334 ± 1.096

1.518 ± 0.247

5.271 ± 0.248

5.426 ± 0.647

6.86 ± 0.334

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.938 ± 0.393

5.117 ± 0.397

4.189 ± 0.214

3.655 ± 0.201

5.342 ± 0.4

6.719 ± 0.536

5.777 ± 0.382

6.846 ± 0.47

2.08 ± 0.186

3.458 ± 0.205
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski