Hubei diptera virus 18

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLM4|A0A1L3KLM4_9VIRU RdRp OS=Hubei diptera virus 18 OX=1922879 PE=4 SV=1
MM1 pKa = 7.45SRR3 pKa = 11.84YY4 pKa = 9.58NFNRR8 pKa = 11.84GAPRR12 pKa = 11.84GRR14 pKa = 11.84GGQWRR19 pKa = 11.84APQNNNYY26 pKa = 9.76DD27 pKa = 2.98AGYY30 pKa = 9.8YY31 pKa = 9.6QNQNQNNRR39 pKa = 11.84SNNNYY44 pKa = 9.52PEE46 pKa = 4.47NNSYY50 pKa = 9.82MYY52 pKa = 8.6EE53 pKa = 4.07APQTSGRR60 pKa = 11.84GRR62 pKa = 11.84GRR64 pKa = 11.84GGYY67 pKa = 9.33RR68 pKa = 11.84RR69 pKa = 11.84GGISNDD75 pKa = 2.9PKK77 pKa = 10.88VNVRR81 pKa = 11.84NPLDD85 pKa = 3.82AKK87 pKa = 11.21DD88 pKa = 4.05PVAQGKK94 pKa = 7.16PASMPTGQHH103 pKa = 5.48LSKK106 pKa = 10.12TNSEE110 pKa = 4.5LKK112 pKa = 10.42NDD114 pKa = 4.26PLYY117 pKa = 11.34LEE119 pKa = 5.42AFFPSPSEE127 pKa = 3.8EE128 pKa = 3.93LVPLPEE134 pKa = 4.38EE135 pKa = 4.45LNHH138 pKa = 5.71FTGFDD143 pKa = 3.57GLSSIINEE151 pKa = 4.47SYY153 pKa = 11.68DD154 pKa = 3.58NFCSHH159 pKa = 6.66SLNFKK164 pKa = 10.36RR165 pKa = 11.84SVSLSSYY172 pKa = 10.34AYY174 pKa = 9.8YY175 pKa = 10.27ISVFCWARR183 pKa = 11.84VLHH186 pKa = 6.5LKK188 pKa = 10.27RR189 pKa = 11.84LNKK192 pKa = 9.77YY193 pKa = 10.05RR194 pKa = 11.84LTTNEE199 pKa = 3.76IEE201 pKa = 4.37FVDD204 pKa = 4.28MIYY207 pKa = 10.9NQGNYY212 pKa = 10.15LLPKK216 pKa = 10.31SVTLYY221 pKa = 11.1LSGFGNFTIPSSVEE235 pKa = 3.94SKK237 pKa = 11.04FNTKK241 pKa = 9.99PYY243 pKa = 10.29SYY245 pKa = 10.99DD246 pKa = 3.23DD247 pKa = 3.22AGYY250 pKa = 9.37FVNFAEE256 pKa = 4.32EE257 pKa = 5.2FYY259 pKa = 10.67TVTSYY264 pKa = 11.36PCISIFAEE272 pKa = 4.24RR273 pKa = 11.84IMRR276 pKa = 11.84DD277 pKa = 3.04MAFTEE282 pKa = 4.01NNRR285 pKa = 11.84VGEE288 pKa = 4.11AWSPNDD294 pKa = 5.67IEE296 pKa = 5.04QEE298 pKa = 3.98WNTRR302 pKa = 11.84CLGYY306 pKa = 10.65APSVEE311 pKa = 4.38LPSLQLQILNMAGISSEE328 pKa = 4.19NFPSDD333 pKa = 4.83CEE335 pKa = 3.97GLLINIRR342 pKa = 11.84LLNSIQKK349 pKa = 9.58YY350 pKa = 9.8LLEE353 pKa = 4.67IPSFEE358 pKa = 4.69SGPIPANLTGSLGQFVVEE376 pKa = 4.04EE377 pKa = 4.26VVEE380 pKa = 4.09NAVALDD386 pKa = 4.56AIDD389 pKa = 4.77NIGSASFITKK399 pKa = 10.21SPLSCPGSLSYY410 pKa = 11.08LGGSFLYY417 pKa = 10.14RR418 pKa = 11.84VAKK421 pKa = 10.07KK422 pKa = 10.21ALPLNKK428 pKa = 9.41IKK430 pKa = 10.7FFFPYY435 pKa = 9.48TINDD439 pKa = 3.34INQLDD444 pKa = 4.12INRR447 pKa = 11.84LDD449 pKa = 3.86TLNHH453 pKa = 5.58GWSPLLEE460 pKa = 4.66NIYY463 pKa = 10.39HH464 pKa = 5.8YY465 pKa = 11.54SNVQFKK471 pKa = 10.57PILRR475 pKa = 11.84LKK477 pKa = 9.61KK478 pKa = 9.99FCSIDD483 pKa = 3.54VKK485 pKa = 9.95PTSVV489 pKa = 2.87

Molecular weight: 55.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLM4|A0A1L3KLM4_9VIRU RdRp OS=Hubei diptera virus 18 OX=1922879 PE=4 SV=1
MM1 pKa = 7.69RR2 pKa = 11.84NVTKK6 pKa = 10.5AIRR9 pKa = 11.84EE10 pKa = 3.95LAPAQGYY17 pKa = 8.19VMNMTPARR25 pKa = 11.84EE26 pKa = 4.02DD27 pKa = 3.03GKK29 pKa = 11.15ARR31 pKa = 11.84LACEE35 pKa = 4.08LFFGKK40 pKa = 10.37DD41 pKa = 3.01KK42 pKa = 11.05VDD44 pKa = 3.2KK45 pKa = 9.91VTNTLHH51 pKa = 7.69RR52 pKa = 11.84SRR54 pKa = 11.84LNYY57 pKa = 10.34DD58 pKa = 3.39SLIEE62 pKa = 5.22DD63 pKa = 3.67FLEE66 pKa = 3.8YY67 pKa = 10.84DD68 pKa = 3.37RR69 pKa = 11.84DD70 pKa = 3.59HH71 pKa = 6.52VRR73 pKa = 11.84RR74 pKa = 11.84FEE76 pKa = 5.04DD77 pKa = 4.18PDD79 pKa = 3.5TKK81 pKa = 10.9LIYY84 pKa = 9.72NTVLQSIIDD93 pKa = 3.84DD94 pKa = 4.79LGDD97 pKa = 3.55QRR99 pKa = 11.84IKK101 pKa = 10.82PLTFDD106 pKa = 3.27QVRR109 pKa = 11.84NLSDD113 pKa = 3.65FPGAKK118 pKa = 9.96SPGLPYY124 pKa = 10.51KK125 pKa = 9.97NQGYY129 pKa = 9.22RR130 pKa = 11.84SKK132 pKa = 11.63AEE134 pKa = 3.96VYY136 pKa = 9.85DD137 pKa = 3.75CKK139 pKa = 11.19EE140 pKa = 3.92NLEE143 pKa = 4.12HH144 pKa = 7.59LEE146 pKa = 4.52NIWDD150 pKa = 4.75LIGNGKK156 pKa = 8.95NVYY159 pKa = 10.35LPDD162 pKa = 3.08VCLFARR168 pKa = 11.84SQIAKK173 pKa = 9.71YY174 pKa = 9.24PKK176 pKa = 9.64EE177 pKa = 4.41KK178 pKa = 10.08IRR180 pKa = 11.84ATWGYY185 pKa = 10.4SFDD188 pKa = 3.69VYY190 pKa = 10.53MEE192 pKa = 4.19EE193 pKa = 4.97ARR195 pKa = 11.84FFYY198 pKa = 10.03PIQEE202 pKa = 4.4FIKK205 pKa = 9.75SHH207 pKa = 4.53KK208 pKa = 9.57HH209 pKa = 5.21KK210 pKa = 11.07LPIAYY215 pKa = 9.27GLEE218 pKa = 4.15MAHH221 pKa = 6.94GGMNSINDD229 pKa = 3.38MLLRR233 pKa = 11.84NRR235 pKa = 11.84GCKK238 pKa = 9.87YY239 pKa = 10.8VISDD243 pKa = 3.14WSKK246 pKa = 9.92FDD248 pKa = 3.25KK249 pKa = 10.72TIPPWLIRR257 pKa = 11.84DD258 pKa = 3.75AFHH261 pKa = 7.92ILEE264 pKa = 4.31KK265 pKa = 10.92LIDD268 pKa = 4.12FEE270 pKa = 4.7SLPRR274 pKa = 11.84PAYY277 pKa = 8.42QRR279 pKa = 11.84RR280 pKa = 11.84RR281 pKa = 11.84FKK283 pKa = 10.93KK284 pKa = 8.17IVDD287 pKa = 3.94YY288 pKa = 10.66FVEE291 pKa = 4.67TPIRR295 pKa = 11.84TCKK298 pKa = 10.63GEE300 pKa = 3.98RR301 pKa = 11.84FLVTGGVPSGSCFTNIIDD319 pKa = 5.23SIINCIVTRR328 pKa = 11.84FLVYY332 pKa = 9.27QTTNQFPIGEE342 pKa = 4.44IFLGDD347 pKa = 3.59DD348 pKa = 3.73GVFVISGFACLEE360 pKa = 4.8DD361 pKa = 3.6IASLAIKK368 pKa = 9.83YY369 pKa = 10.19FGMILNVDD377 pKa = 3.4KK378 pKa = 11.19SYY380 pKa = 10.2VTTNPQNVHH389 pKa = 5.18FLGYY393 pKa = 10.06FNYY396 pKa = 10.91SGMPFKK402 pKa = 11.23NQDD405 pKa = 3.14FLIASFIFPEE415 pKa = 4.29HH416 pKa = 6.37KK417 pKa = 9.12RR418 pKa = 11.84TRR420 pKa = 11.84LIDD423 pKa = 3.39ACAAALGQMYY433 pKa = 10.5SGFDD437 pKa = 3.16PGYY440 pKa = 10.04ARR442 pKa = 11.84TWLKK446 pKa = 10.21IIHH449 pKa = 6.09YY450 pKa = 10.03LADD453 pKa = 3.98AEE455 pKa = 4.33NANPFNFHH463 pKa = 6.93EE464 pKa = 4.73IVLHH468 pKa = 5.73LRR470 pKa = 11.84NNEE473 pKa = 3.66FRR475 pKa = 11.84HH476 pKa = 6.22KK477 pKa = 10.72YY478 pKa = 8.54LAQVGLTSDD487 pKa = 2.95NMTIPEE493 pKa = 4.48LHH495 pKa = 6.74SSMILEE501 pKa = 4.47VLPKK505 pKa = 9.8SYY507 pKa = 10.73CAISLPNRR515 pKa = 11.84TYY517 pKa = 10.81DD518 pKa = 3.51YY519 pKa = 10.04KK520 pKa = 11.07SLYY523 pKa = 9.48IRR525 pKa = 11.84SLSSFPP531 pKa = 4.25

Molecular weight: 61.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

1020

489

531

510.0

58.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.294 ± 0.217

1.667 ± 0.132

5.294 ± 0.79

5.392 ± 0.187

6.078 ± 0.312

5.98 ± 0.316

1.961 ± 0.412

6.863 ± 0.638

5.294 ± 0.561

9.216 ± 0.107

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.863 ± 0.242

7.549 ± 1.271

5.784 ± 0.426

2.941 ± 0.186

5.49 ± 0.327

7.843 ± 1.221

4.02 ± 0.19

4.804 ± 0.056

0.98 ± 0.024

5.686 ± 0.137
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski