Amino acid dipepetide frequency for Puccinia sorghi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	4.949AlaAla: 4.949 ± 0.035	1.266AlaCys: 1.266 ± 0.014	2.688AlaAsp: 2.688 ± 0.019	3.294AlaGlu: 3.294 ± 0.029	2.353AlaPhe: 2.353 ± 0.018	3.595AlaGly: 3.595 ± 0.029	1.839AlaHis: 1.839 ± 0.017	3.302AlaIle: 3.302 ± 0.024	3.303AlaLys: 3.303 ± 0.023	5.847AlaLeu: 5.847 ± 0.029	1.312AlaMet: 1.312 ± 0.013	2.516AlaAsn: 2.516 ± 0.019	3.617AlaPro: 3.617 ± 0.031	2.733AlaGln: 2.733 ± 0.02	3.284AlaArg: 3.284 ± 0.024	5.973AlaSer: 5.973 ± 0.036	3.765AlaThr: 3.765 ± 0.027	3.247AlaVal: 3.247 ± 0.024	0.801AlaTrp: 0.801 ± 0.012	1.438AlaTyr: 1.438 ± 0.015	0.001AlaXaa: 0.001 ± 0.0
Cys	1.052CysAla: 1.052 ± 0.012	0.717CysCys: 0.717 ± 0.012	0.723CysAsp: 0.723 ± 0.011	0.749CysGlu: 0.749 ± 0.012	1.271CysPhe: 1.271 ± 0.015	1.232CysGly: 1.232 ± 0.016	0.747CysHis: 0.747 ± 0.012	1.096CysIle: 1.096 ± 0.013	1.048CysLys: 1.048 ± 0.013	2.443CysLeu: 2.443 ± 0.022	0.486CysMet: 0.486 ± 0.008	0.808CysAsn: 0.808 ± 0.012	1.094CysPro: 1.094 ± 0.014	0.978CysGln: 0.978 ± 0.012	1.107CysArg: 1.107 ± 0.014	1.91CysSer: 1.91 ± 0.018	1.074CysThr: 1.074 ± 0.013	1.084CysVal: 1.084 ± 0.012	0.413CysTrp: 0.413 ± 0.007	0.616CysTyr: 0.616 ± 0.012	0.001CysXaa: 0.001 ± 0.0
Asp	2.528AspAla: 2.528 ± 0.022	0.834AspCys: 0.834 ± 0.011	2.642AspAsp: 2.642 ± 0.029	2.813AspGlu: 2.813 ± 0.027	1.907AspPhe: 1.907 ± 0.015	2.582AspGly: 2.582 ± 0.023	1.483AspHis: 1.483 ± 0.015	2.124AspIle: 2.124 ± 0.018	2.117AspLys: 2.117 ± 0.019	4.545AspLeu: 4.545 ± 0.027	0.882AspMet: 0.882 ± 0.011	1.756AspAsn: 1.756 ± 0.018	2.817AspPro: 2.817 ± 0.024	2.177AspGln: 2.177 ± 0.019	2.169AspArg: 2.169 ± 0.022	3.908AspSer: 3.908 ± 0.029	2.017AspThr: 2.017 ± 0.015	2.269AspVal: 2.269 ± 0.019	0.721AspTrp: 0.721 ± 0.01	1.19AspTyr: 1.19 ± 0.012	0.001AspXaa: 0.001 ± 0.0
Glu	3.456GluAla: 3.456 ± 0.026	0.758GluCys: 0.758 ± 0.011	2.768GluAsp: 2.768 ± 0.028	4.178GluGlu: 4.178 ± 0.038	1.899GluPhe: 1.899 ± 0.018	2.694GluGly: 2.694 ± 0.019	1.165GluHis: 1.165 ± 0.012	3.083GluIle: 3.083 ± 0.02	3.414GluLys: 3.414 ± 0.026	4.891GluLeu: 4.891 ± 0.034	1.193GluMet: 1.193 ± 0.013	2.334GluAsn: 2.334 ± 0.019	2.284GluPro: 2.284 ± 0.02	1.995GluGln: 1.995 ± 0.019	2.83GluArg: 2.83 ± 0.025	3.978GluSer: 3.978 ± 0.026	2.618GluThr: 2.618 ± 0.02	2.665GluVal: 2.665 ± 0.024	0.673GluTrp: 0.673 ± 0.009	1.209GluTyr: 1.209 ± 0.013	0.001GluXaa: 0.001 ± 0.0
Phe	2.041PheAla: 2.041 ± 0.019	1.2PheCys: 1.2 ± 0.017	1.911PheAsp: 1.911 ± 0.017	2.05PheGlu: 2.05 ± 0.015	3.631PhePhe: 3.631 ± 0.045	2.385PheGly: 2.385 ± 0.021	1.58PheHis: 1.58 ± 0.017	2.699PheIle: 2.699 ± 0.022	2.35PheLys: 2.35 ± 0.022	5.729PheLeu: 5.729 ± 0.039	0.899PheMet: 0.899 ± 0.012	2.139PheAsn: 2.139 ± 0.02	2.606PhePro: 2.606 ± 0.019	2.047PheGln: 2.047 ± 0.019	1.866PheArg: 1.866 ± 0.016	4.464PheSer: 4.464 ± 0.033	2.134PheThr: 2.134 ± 0.017	2.198PheVal: 2.198 ± 0.017	0.803PheTrp: 0.803 ± 0.012	1.372PheTyr: 1.372 ± 0.017	0.002PheXaa: 0.002 ± 0.0
Gly	3.154GlyAla: 3.154 ± 0.025	1.176GlyCys: 1.176 ± 0.013	2.124GlyAsp: 2.124 ± 0.02	2.534GlyGlu: 2.534 ± 0.021	2.476GlyPhe: 2.476 ± 0.023	4.032GlyGly: 4.032 ± 0.042	1.535GlyHis: 1.535 ± 0.016	3.037GlyIle: 3.037 ± 0.023	3.266GlyLys: 3.266 ± 0.024	5.374GlyLeu: 5.374 ± 0.033	1.245GlyMet: 1.245 ± 0.014	2.206GlyAsn: 2.206 ± 0.017	2.6GlyPro: 2.6 ± 0.022	2.112GlyGln: 2.112 ± 0.016	3.039GlyArg: 3.039 ± 0.024	4.899GlySer: 4.899 ± 0.035	2.925GlyThr: 2.925 ± 0.024	2.954GlyVal: 2.954 ± 0.022	0.909GlyTrp: 0.909 ± 0.012	1.488GlyTyr: 1.488 ± 0.017	0.001GlyXaa: 0.001 ± 0.0
His	1.634HisAla: 1.634 ± 0.015	0.761HisCys: 0.761 ± 0.01	1.172HisAsp: 1.172 ± 0.013	1.269HisGlu: 1.269 ± 0.015	1.552HisPhe: 1.552 ± 0.017	1.385HisGly: 1.385 ± 0.014	1.772HisHis: 1.772 ± 0.026	1.695HisIle: 1.695 ± 0.016	1.462HisLys: 1.462 ± 0.014	3.749HisLeu: 3.749 ± 0.026	0.604HisMet: 0.604 ± 0.008	1.45HisAsn: 1.45 ± 0.015	2.563HisPro: 2.563 ± 0.021	1.951HisGln: 1.951 ± 0.019	1.611HisArg: 1.611 ± 0.017	3.244HisSer: 3.244 ± 0.024	1.7HisThr: 1.7 ± 0.014	1.54HisVal: 1.54 ± 0.014	0.494HisTrp: 0.494 ± 0.008	0.82HisTyr: 0.82 ± 0.011	0.001HisXaa: 0.001 ± 0.0
Ile	2.825IleAla: 2.825 ± 0.018	1.275IleCys: 1.275 ± 0.015	2.538IleAsp: 2.538 ± 0.021	2.74IleGlu: 2.74 ± 0.021	3.032IlePhe: 3.032 ± 0.029	2.776IleGly: 2.776 ± 0.019	1.911IleHis: 1.911 ± 0.016	3.582IleIle: 3.582 ± 0.028	3.653IleLys: 3.653 ± 0.027	6.219IleLeu: 6.219 ± 0.032	1.119IleMet: 1.119 ± 0.014	2.983IleAsn: 2.983 ± 0.024	3.448IlePro: 3.448 ± 0.023	2.758IleGln: 2.758 ± 0.02	2.625IleArg: 2.625 ± 0.02	5.468IleSer: 5.468 ± 0.033	3.193IleThr: 3.193 ± 0.022	2.651IleVal: 2.651 ± 0.023	0.84IleTrp: 0.84 ± 0.011	1.762IleTyr: 1.762 ± 0.017	0.001IleXaa: 0.001 ± 0.0
Lys	3.623LysAla: 3.623 ± 0.024	0.932LysCys: 0.932 ± 0.013	2.349LysAsp: 2.349 ± 0.022	3.282LysGlu: 3.282 ± 0.026	2.362LysPhe: 2.362 ± 0.021	2.818LysGly: 2.818 ± 0.021	1.607LysHis: 1.607 ± 0.017	3.803LysIle: 3.803 ± 0.027	5.321LysLys: 5.321 ± 0.046	5.898LysLeu: 5.898 ± 0.033	1.43LysMet: 1.43 ± 0.015	3.116LysAsn: 3.116 ± 0.025	3.248LysPro: 3.248 ± 0.022	2.478LysGln: 2.478 ± 0.02	3.213LysArg: 3.213 ± 0.022	4.78LysSer: 4.78 ± 0.029	3.588LysThr: 3.588 ± 0.024	2.795LysVal: 2.795 ± 0.022	0.736LysTrp: 0.736 ± 0.01	1.588LysTyr: 1.588 ± 0.015	0.002LysXaa: 0.002 ± 0.0
Leu	6.814LeuAla: 6.814 ± 0.032	2.127LeuCys: 2.127 ± 0.022	4.815LeuAsp: 4.815 ± 0.03	5.197LeuGlu: 5.197 ± 0.032	4.847LeuPhe: 4.847 ± 0.038	5.258LeuGly: 5.258 ± 0.029	3.213LeuHis: 3.213 ± 0.021	6.226LeuIle: 6.226 ± 0.037	6.361LeuLys: 6.361 ± 0.036	10.695LeuLeu: 10.695 ± 0.053	2.166LeuMet: 2.166 ± 0.019	4.954LeuAsn: 4.954 ± 0.03	6.351LeuPro: 6.351 ± 0.036	4.391LeuGln: 4.391 ± 0.028	5.131LeuArg: 5.131 ± 0.03	9.667LeuSer: 9.667 ± 0.044	5.797LeuThr: 5.797 ± 0.028	5.762LeuVal: 5.762 ± 0.03	1.357LeuTrp: 1.357 ± 0.014	2.535LeuTyr: 2.535 ± 0.02	0.004LeuXaa: 0.004 ± 0.001
Met	1.568MetAla: 1.568 ± 0.015	0.408MetCys: 0.408 ± 0.009	1.04MetAsp: 1.04 ± 0.013	1.166MetGlu: 1.166 ± 0.013	0.769MetPhe: 0.769 ± 0.01	1.198MetGly: 1.198 ± 0.014	0.53MetHis: 0.53 ± 0.01	1.41MetIle: 1.41 ± 0.015	1.402MetLys: 1.402 ± 0.014	1.885MetLeu: 1.885 ± 0.018	0.663MetMet: 0.663 ± 0.012	1.022MetAsn: 1.022 ± 0.011	0.979MetPro: 0.979 ± 0.013	0.806MetGln: 0.806 ± 0.011	1.093MetArg: 1.093 ± 0.014	1.875MetSer: 1.875 ± 0.016	1.239MetThr: 1.239 ± 0.012	1.278MetVal: 1.278 ± 0.013	0.304MetTrp: 0.304 ± 0.006	0.502MetTyr: 0.502 ± 0.009	0.0MetXaa: 0.0 ± 0.0
Asn	2.169AsnAla: 2.169 ± 0.022	0.955AsnCys: 0.955 ± 0.012	1.724AsnAsp: 1.724 ± 0.016	1.945AsnGlu: 1.945 ± 0.019	2.184AsnPhe: 2.184 ± 0.019	2.233AsnGly: 2.233 ± 0.019	1.907AsnHis: 1.907 ± 0.019	2.648AsnIle: 2.648 ± 0.024	2.631AsnLys: 2.631 ± 0.022	5.095AsnLeu: 5.095 ± 0.028	0.931AsnMet: 0.931 ± 0.011	2.595AsnAsn: 2.595 ± 0.029	3.401AsnPro: 3.401 ± 0.022	2.711AsnGln: 2.711 ± 0.022	2.195AsnArg: 2.195 ± 0.017	4.497AsnSer: 4.497 ± 0.026	2.549AsnThr: 2.549 ± 0.02	2.059AsnVal: 2.059 ± 0.018	0.677AsnTrp: 0.677 ± 0.01	1.431AsnTyr: 1.431 ± 0.015	0.001AsnXaa: 0.001 ± 0.0
Pro	4.217ProAla: 4.217 ± 0.032	1.023ProCys: 1.023 ± 0.012	2.55ProAsp: 2.55 ± 0.02	2.79ProGlu: 2.79 ± 0.022	2.416ProPhe: 2.416 ± 0.017	3.004ProGly: 3.004 ± 0.023	2.05ProHis: 2.05 ± 0.018	3.243ProIle: 3.243 ± 0.024	3.037ProLys: 3.037 ± 0.024	5.832ProLeu: 5.832 ± 0.031	1.057ProMet: 1.057 ± 0.012	2.882ProAsn: 2.882 ± 0.023	5.553ProPro: 5.553 ± 0.054	2.809ProGln: 2.809 ± 0.024	2.851ProArg: 2.851 ± 0.024	7.25ProSer: 7.25 ± 0.048	4.28ProThr: 4.28 ± 0.031	3.037ProVal: 3.037 ± 0.022	0.655ProTrp: 0.655 ± 0.01	1.252ProTyr: 1.252 ± 0.014	0.002ProXaa: 0.002 ± 0.001
Gln	3.239GlnAla: 3.239 ± 0.025	0.734GlnCys: 0.734 ± 0.01	2.034GlnAsp: 2.034 ± 0.017	2.441GlnGlu: 2.441 ± 0.02	1.729GlnPhe: 1.729 ± 0.015	2.046GlnGly: 2.046 ± 0.017	1.576GlnHis: 1.576 ± 0.015	2.645GlnIle: 2.645 ± 0.02	2.678GlnLys: 2.678 ± 0.02	4.782GlnLeu: 4.782 ± 0.027	0.953GlnMet: 0.953 ± 0.013	2.08GlnAsn: 2.08 ± 0.021	3.108GlnPro: 3.108 ± 0.025	2.66GlnGln: 2.66 ± 0.032	2.35GlnArg: 2.35 ± 0.019	4.147GlnSer: 4.147 ± 0.027	2.671GlnThr: 2.671 ± 0.022	2.444GlnVal: 2.444 ± 0.018	0.575GlnTrp: 0.575 ± 0.009	1.037GlnTyr: 1.037 ± 0.013	0.001GlnXaa: 0.001 ± 0.0
Arg	3.2ArgAla: 3.2 ± 0.026	1.004ArgCys: 1.004 ± 0.012	2.092ArgAsp: 2.092 ± 0.019	2.566ArgGlu: 2.566 ± 0.021	2.153ArgPhe: 2.153 ± 0.017	2.835ArgGly: 2.835 ± 0.022	1.455ArgHis: 1.455 ± 0.017	2.888ArgIle: 2.888 ± 0.022	3.457ArgLys: 3.457 ± 0.023	5.301ArgLeu: 5.301 ± 0.033	1.164ArgMet: 1.164 ± 0.013	2.315ArgAsn: 2.315 ± 0.019	2.99ArgPro: 2.99 ± 0.024	2.312ArgGln: 2.312 ± 0.02	3.782ArgArg: 3.782 ± 0.033	4.506ArgSer: 4.506 ± 0.036	2.919ArgThr: 2.919 ± 0.021	2.63ArgVal: 2.63 ± 0.019	0.777ArgTrp: 0.777 ± 0.01	1.299ArgTyr: 1.299 ± 0.013	0.002ArgXaa: 0.002 ± 0.0
Ser	5.652SerAla: 5.652 ± 0.038	2.061SerCys: 2.061 ± 0.021	3.83SerAsp: 3.83 ± 0.025	3.869SerGlu: 3.869 ± 0.024	4.562SerPhe: 4.562 ± 0.028	4.834SerGly: 4.834 ± 0.03	3.196SerHis: 3.196 ± 0.02	5.219SerIle: 5.219 ± 0.031	5.175SerLys: 5.175 ± 0.034	9.863SerLeu: 9.863 ± 0.042	1.884SerMet: 1.884 ± 0.013	4.685SerAsn: 4.685 ± 0.026	6.156SerPro: 6.156 ± 0.042	4.462SerGln: 4.462 ± 0.028	4.927SerArg: 4.927 ± 0.039	12.284SerSer: 12.284 ± 0.081	6.362SerThr: 6.362 ± 0.034	4.323SerVal: 4.323 ± 0.026	1.264SerTrp: 1.264 ± 0.014	2.245SerTyr: 2.245 ± 0.018	0.003SerXaa: 0.003 ± 0.001
Thr	3.608ThrAla: 3.608 ± 0.026	1.325ThrCys: 1.325 ± 0.015	2.191ThrAsp: 2.191 ± 0.018	2.46ThrGlu: 2.46 ± 0.02	2.368ThrPhe: 2.368 ± 0.02	3.132ThrGly: 3.132 ± 0.025	2.031ThrHis: 2.031 ± 0.02	3.304ThrIle: 3.304 ± 0.022	3.116ThrLys: 3.116 ± 0.025	5.658ThrLeu: 5.658 ± 0.027	1.104ThrMet: 1.104 ± 0.012	2.67ThrAsn: 2.67 ± 0.021	4.058ThrPro: 4.058 ± 0.036	2.655ThrGln: 2.655 ± 0.02	3.089ThrArg: 3.089 ± 0.023	5.923ThrSer: 5.923 ± 0.034	4.221ThrThr: 4.221 ± 0.033	2.77ThrVal: 2.77 ± 0.019	0.828ThrTrp: 0.828 ± 0.011	1.427ThrTyr: 1.427 ± 0.014	0.001ThrXaa: 0.001 ± 0.0
Val	3.333ValAla: 3.333 ± 0.022	1.127ValCys: 1.127 ± 0.013	2.56ValAsp: 2.56 ± 0.023	2.958ValGlu: 2.958 ± 0.023	2.467ValPhe: 2.467 ± 0.02	2.959ValGly: 2.959 ± 0.021	1.397ValHis: 1.397 ± 0.013	2.894ValIle: 2.894 ± 0.023	2.83ValLys: 2.83 ± 0.02	5.13ValLeu: 5.13 ± 0.032	1.119ValMet: 1.119 ± 0.011	2.086ValAsn: 2.086 ± 0.016	2.805ValPro: 2.805 ± 0.023	2.078ValGln: 2.078 ± 0.016	2.417ValArg: 2.417 ± 0.017	4.585ValSer: 4.585 ± 0.025	2.817ValThr: 2.817 ± 0.021	3.315ValVal: 3.315 ± 0.025	0.848ValTrp: 0.848 ± 0.011	1.319ValTyr: 1.319 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.813TrpAla: 0.813 ± 0.011	0.343TrpCys: 0.343 ± 0.007	0.657TrpAsp: 0.657 ± 0.01	0.743TrpGlu: 0.743 ± 0.011	0.593TrpPhe: 0.593 ± 0.009	0.797TrpGly: 0.797 ± 0.012	0.432TrpHis: 0.432 ± 0.008	0.898TrpIle: 0.898 ± 0.012	1.089TrpLys: 1.089 ± 0.014	1.543TrpLeu: 1.543 ± 0.014	0.381TrpMet: 0.381 ± 0.008	0.776TrpAsn: 0.776 ± 0.011	0.626TrpPro: 0.626 ± 0.01	0.54TrpGln: 0.54 ± 0.009	0.84TrpArg: 0.84 ± 0.01	1.139TrpSer: 1.139 ± 0.013	0.735TrpThr: 0.735 ± 0.01	0.767TrpVal: 0.767 ± 0.011	0.263TrpTrp: 0.263 ± 0.006	0.353TrpTyr: 0.353 ± 0.008	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.247TyrAla: 1.247 ± 0.014	0.621TyrCys: 0.621 ± 0.009	1.072TyrAsp: 1.072 ± 0.014	1.038TyrGlu: 1.038 ± 0.012	1.531TyrPhe: 1.531 ± 0.018	1.291TyrGly: 1.291 ± 0.017	1.003TyrHis: 1.003 ± 0.012	1.6TyrIle: 1.6 ± 0.018	1.257TyrLys: 1.257 ± 0.016	3.186TyrLeu: 3.186 ± 0.023	0.549TyrMet: 0.549 ± 0.008	1.164TyrAsn: 1.164 ± 0.013	1.485TyrPro: 1.485 ± 0.015	1.275TyrGln: 1.275 ± 0.012	1.225TyrArg: 1.225 ± 0.012	2.356TyrSer: 2.356 ± 0.019	1.334TyrThr: 1.334 ± 0.013	1.257TyrVal: 1.257 ± 0.015	0.391TyrTrp: 0.391 ± 0.008	0.854TyrTyr: 0.854 ± 0.011	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.001XaaAla: 0.001 ± 0.0	0.001XaaCys: 0.001 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.002XaaPhe: 0.002 ± 0.0	0.002XaaGly: 0.002 ± 0.0	0.001XaaHis: 0.001 ± 0.0	0.001XaaIle: 0.001 ± 0.0	0.002XaaLys: 0.002 ± 0.001	0.003XaaLeu: 0.003 ± 0.001	0.001XaaMet: 0.001 ± 0.0	0.001XaaAsn: 0.001 ± 0.0	0.002XaaPro: 0.002 ± 0.0	0.001XaaGln: 0.001 ± 0.0	0.002XaaArg: 0.002 ± 0.0	0.003XaaSer: 0.003 ± 0.001	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.0	0.001XaaTyr: 0.001 ± 0.0	0.026XaaXaa: 0.026 ± 0.006

Statistics based on 21032 proteins (7432697 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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