Cellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Cellvibrionales; Cellvibrionaceae; Cellvibrio; Cellvibrio japonicus

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3711 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B3PL35|B3PL35_CELJU Shikimate kinase OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=aroK PE=3 SV=1
MM1 pKa = 7.62AEE3 pKa = 3.73AAFLEE8 pKa = 4.59KK9 pKa = 10.62SSNIYY14 pKa = 10.85ALVGSQTNDD23 pKa = 2.94LFDD26 pKa = 4.15VDD28 pKa = 4.5FQSVGAEE35 pKa = 3.72FYY37 pKa = 10.42IPNSIFYY44 pKa = 10.32AGLGVTRR51 pKa = 11.84AKK53 pKa = 10.93ADD55 pKa = 3.44GGSWDD60 pKa = 4.07SQWNASLGVTPIDD73 pKa = 4.12GLLVWTSFYY82 pKa = 11.28EE83 pKa = 4.18DD84 pKa = 3.3VDD86 pKa = 3.78YY87 pKa = 11.7SDD89 pKa = 5.51YY90 pKa = 11.56INLNAKK96 pKa = 9.94YY97 pKa = 10.27VMSLGGEE104 pKa = 3.72QALNIEE110 pKa = 4.73GSFADD115 pKa = 3.57ADD117 pKa = 3.76GGEE120 pKa = 4.34EE121 pKa = 3.81FGIAADD127 pKa = 3.93YY128 pKa = 11.15YY129 pKa = 10.18IDD131 pKa = 4.13RR132 pKa = 11.84NLSIGTAYY140 pKa = 10.65SYY142 pKa = 11.99VNADD146 pKa = 3.3EE147 pKa = 5.28DD148 pKa = 3.72IKK150 pKa = 11.45DD151 pKa = 3.46FTVRR155 pKa = 11.84ARR157 pKa = 11.84QFFTNSLSFDD167 pKa = 3.66LSYY170 pKa = 11.3TFGDD174 pKa = 4.16QDD176 pKa = 4.06DD177 pKa = 3.8SWGIGASYY185 pKa = 10.75RR186 pKa = 11.84FF187 pKa = 3.67

Molecular weight: 20.62 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B3PJF7|B3PJF7_CELJU Uncharacterized protein OS=Cellvibrio japonicus (strain Ueda107) OX=498211 GN=CJA_2269 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNIKK11 pKa = 9.97RR12 pKa = 11.84VRR14 pKa = 11.84NHH16 pKa = 5.5GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.35NGRR28 pKa = 11.84LVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.15GRR39 pKa = 11.84KK40 pKa = 8.02QLTRR44 pKa = 11.84VV45 pKa = 3.2

Molecular weight: 5.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3711

0

3711

1367215

30

12743

368.4

40.72

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.673 ± 0.047

0.966 ± 0.016

5.528 ± 0.039

5.501 ± 0.042

3.794 ± 0.025

7.341 ± 0.049

2.308 ± 0.024

5.665 ± 0.03

3.982 ± 0.035

10.679 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.17 ± 0.027

4.096 ± 0.044

4.582 ± 0.027

4.755 ± 0.037

5.566 ± 0.037

6.538 ± 0.053

5.383 ± 0.057

6.792 ± 0.044

1.491 ± 0.017

3.187 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski