Leuconostoc phage LN04

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Mccleskeyvirinae; Limdunavirus; Leuconostoc virus LN04

Average proteome isoelectric point is 6.43

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 39 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4I6C0|M4I6C0_9CAUD Putative phage tail tape measure protein OS=Leuconostoc phage LN04 OX=1262516 GN=phiLN04_020 PE=4 SV=1
MM1 pKa = 7.0TTFDD5 pKa = 3.51YY6 pKa = 10.99QKK8 pKa = 10.76YY9 pKa = 10.68LNVDD13 pKa = 3.38DD14 pKa = 5.04DD15 pKa = 4.92TYY17 pKa = 11.61SDD19 pKa = 3.04WSGTIEE25 pKa = 4.08EE26 pKa = 5.23LEE28 pKa = 3.87NSAINRR34 pKa = 11.84AQRR37 pKa = 11.84LNPTLTIDD45 pKa = 3.63TTNKK49 pKa = 10.17NLYY52 pKa = 9.14EE53 pKa = 4.06YY54 pKa = 9.58AKK56 pKa = 9.37EE57 pKa = 3.94VIYY60 pKa = 10.55IGIFGDD66 pKa = 3.89ALTPALWQYY75 pKa = 11.21HH76 pKa = 5.65DD77 pKa = 4.64SKK79 pKa = 11.05MSDD82 pKa = 2.97LEE84 pKa = 4.45EE85 pKa = 4.19YY86 pKa = 9.86FRR88 pKa = 11.84WWQEE92 pKa = 3.34

Molecular weight: 11.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4I6N7|M4I6N7_9CAUD Receptor-binding tail protein OS=Leuconostoc phage LN04 OX=1262516 GN=phiLN04_023 PE=4 SV=1
MM1 pKa = 7.24VKK3 pKa = 9.98RR4 pKa = 11.84VNSIVDD10 pKa = 3.2IANGTKK16 pKa = 9.92KK17 pKa = 10.2FAIAKK22 pKa = 9.36VLEE25 pKa = 4.47KK26 pKa = 10.02RR27 pKa = 11.84DD28 pKa = 3.47KK29 pKa = 10.01TAQQASSQSNSALGVWHH46 pKa = 6.75GMAPKK51 pKa = 10.47GRR53 pKa = 11.84TGQMVSGVSVKK64 pKa = 10.1HH65 pKa = 5.14GKK67 pKa = 9.88YY68 pKa = 10.06YY69 pKa = 10.37SVVYY73 pKa = 7.6PTHH76 pKa = 7.23DD77 pKa = 3.48YY78 pKa = 10.72RR79 pKa = 11.84RR80 pKa = 11.84EE81 pKa = 3.77GTAFMEE87 pKa = 4.56IVNVQNKK94 pKa = 9.62RR95 pKa = 11.84GVHH98 pKa = 5.45AGFFNRR104 pKa = 11.84YY105 pKa = 5.74RR106 pKa = 11.84TQLGKK111 pKa = 10.74SFLTSVKK118 pKa = 10.6

Molecular weight: 13.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

39

0

39

7893

48

818

202.4

22.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.259 ± 0.459

0.127 ± 0.055

6.322 ± 0.473

4.928 ± 0.53

4.612 ± 0.23

6.651 ± 0.713

1.495 ± 0.223

6.994 ± 0.285

7.12 ± 0.464

8.311 ± 0.291

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.344 ± 0.142

6.791 ± 0.327

3.003 ± 0.212

4.409 ± 0.213

3.332 ± 0.221

7.728 ± 0.61

7.31 ± 0.404

6.803 ± 0.336

1.001 ± 0.157

4.46 ± 0.496
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski