Roseomonas sp. Z24

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Acetobacteraceae; Roseomonas

Average proteome isoelectric point is 6.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5527 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A9JJI7|A0A3A9JJI7_9PROT Mannitol dehydrogenase family protein (Fragment) OS=Roseomonas sp. Z24 OX=2382222 GN=D6Z83_07825 PE=4 SV=1
MM1 pKa = 7.81PNLASPDD8 pKa = 3.71YY9 pKa = 9.83PEE11 pKa = 4.07NAIVLHH17 pKa = 6.64AGDD20 pKa = 4.96NIQEE24 pKa = 4.12AVQAGGEE31 pKa = 4.3GAVFWLEE38 pKa = 3.61AGVYY42 pKa = 9.81RR43 pKa = 11.84MQQITPLEE51 pKa = 4.38GQSFLGASGATLNGARR67 pKa = 11.84LLTEE71 pKa = 4.11FTRR74 pKa = 11.84IDD76 pKa = 3.55GHH78 pKa = 6.08WAAEE82 pKa = 4.14GQTQQGEE89 pKa = 4.13RR90 pKa = 11.84RR91 pKa = 11.84EE92 pKa = 4.14TDD94 pKa = 2.94EE95 pKa = 4.36SAEE98 pKa = 4.1GAQRR102 pKa = 11.84PGYY105 pKa = 9.25PDD107 pKa = 3.05AVYY110 pKa = 11.34VNDD113 pKa = 3.98QPLTPVDD120 pKa = 5.23SIDD123 pKa = 3.42QLKK126 pKa = 10.07PGTYY130 pKa = 10.16FFDD133 pKa = 3.69YY134 pKa = 10.91DD135 pKa = 3.36ADD137 pKa = 4.8RR138 pKa = 11.84IFFGDD143 pKa = 3.95DD144 pKa = 3.04PTGEE148 pKa = 4.22KK149 pKa = 10.49VEE151 pKa = 4.44AAVSPYY157 pKa = 10.8AIASTANDD165 pKa = 3.41VTVSGLTVEE174 pKa = 5.61KK175 pKa = 10.64YY176 pKa = 7.66ATPTQEE182 pKa = 4.24AAISGNGEE190 pKa = 3.61NWVVSNNEE198 pKa = 3.06VRR200 pKa = 11.84LNYY203 pKa = 10.38GVGINVGDD211 pKa = 3.9GGKK214 pKa = 10.06IMDD217 pKa = 4.9NFVHH221 pKa = 7.65DD222 pKa = 4.37NGQMGLNAGGKK233 pKa = 9.55DD234 pKa = 3.36VLVSGNEE241 pKa = 3.66IASNGFWSGIDD252 pKa = 3.63VLWEE256 pKa = 3.95GGGAKK261 pKa = 8.82FTEE264 pKa = 4.73TEE266 pKa = 3.81GLMVRR271 pKa = 11.84NNYY274 pKa = 9.89SHH276 pKa = 7.68DD277 pKa = 3.77NNGYY281 pKa = 9.17GLWTDD286 pKa = 3.01IDD288 pKa = 4.21NVNTTYY294 pKa = 10.52EE295 pKa = 4.29GNRR298 pKa = 11.84IEE300 pKa = 4.19YY301 pKa = 10.37NSAGGINHH309 pKa = 7.44EE310 pKa = 4.09ISYY313 pKa = 10.26AASIHH318 pKa = 6.74DD319 pKa = 3.71NSFVGNGGNGLTWLWGSAIQVQNSQGVEE347 pKa = 3.69IYY349 pKa = 11.26NNFVDD354 pKa = 5.3ASSGGNGIGLIQQEE368 pKa = 4.33RR369 pKa = 11.84GEE371 pKa = 4.52GAFGDD376 pKa = 4.19YY377 pKa = 9.2VTVNNSVHH385 pKa = 7.34DD386 pKa = 3.84NTLILSSEE394 pKa = 4.27SGVGAVADD402 pKa = 3.87YY403 pKa = 11.4DD404 pKa = 4.28EE405 pKa = 5.04EE406 pKa = 5.35LMLAGGNTFNNNEE419 pKa = 4.13YY420 pKa = 10.17QVQADD425 pKa = 3.68EE426 pKa = 4.6DD427 pKa = 3.75QFAFGDD433 pKa = 4.4YY434 pKa = 9.05YY435 pKa = 11.24TFGDD439 pKa = 4.2FVNVSGQDD447 pKa = 2.98AGSALLIGG455 pKa = 4.33

Molecular weight: 48.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A9JI02|A0A3A9JI02_9PROT ABC transporter substrate-binding protein OS=Roseomonas sp. Z24 OX=2382222 GN=D6Z83_09635 PE=4 SV=1
GG1 pKa = 6.95SRR3 pKa = 11.84PRR5 pKa = 11.84PGRR8 pKa = 11.84RR9 pKa = 11.84CPAPPRR15 pKa = 11.84RR16 pKa = 11.84PRR18 pKa = 11.84RR19 pKa = 11.84PRR21 pKa = 11.84RR22 pKa = 11.84RR23 pKa = 11.84SPASIGQKK31 pKa = 9.57RR32 pKa = 11.84RR33 pKa = 11.84PATGGPAKK41 pKa = 10.3ARR43 pKa = 11.84RR44 pKa = 11.84LPAEE48 pKa = 3.87RR49 pKa = 11.84RR50 pKa = 11.84ANAARR55 pKa = 11.84AGRR58 pKa = 11.84GAAWW62 pKa = 3.28

Molecular weight: 6.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5527

0

5527

1684659

25

2457

304.8

32.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.186 ± 0.053

0.834 ± 0.01

4.93 ± 0.019

5.616 ± 0.026

3.313 ± 0.02

9.075 ± 0.025

2.061 ± 0.017

4.106 ± 0.028

2.197 ± 0.024

11.206 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.505 ± 0.015

2.131 ± 0.019

6.18 ± 0.03

3.31 ± 0.019

8.066 ± 0.033

4.785 ± 0.022

4.907 ± 0.019

7.279 ± 0.026

1.477 ± 0.014

1.836 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski