Fusarium redolens polymycovirus 1

Taxonomy: Viruses; Riboviria; Polymycoviridae; Polymycovirus

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A513ZVE3|A0A513ZVE3_9VIRU Uncharacterized protein OS=Fusarium redolens polymycovirus 1 OX=2546034 PE=4 SV=1
MM1 pKa = 7.34SGKK4 pKa = 10.33SFVIQTEE11 pKa = 4.31CEE13 pKa = 4.19GVGRR17 pKa = 11.84RR18 pKa = 11.84GLVSASVMEE27 pKa = 4.68FYY29 pKa = 10.84FIIGVVEE36 pKa = 4.41DD37 pKa = 3.71VCQEE41 pKa = 3.87VATRR45 pKa = 11.84LACLEE50 pKa = 4.31AEE52 pKa = 4.59FKK54 pKa = 11.01EE55 pKa = 4.55SGAVAACPGGWACAGVRR72 pKa = 11.84LVVDD76 pKa = 4.12PASVQVRR83 pKa = 11.84AVSTDD88 pKa = 2.93VSLDD92 pKa = 3.04GSLRR96 pKa = 11.84TLRR99 pKa = 11.84CKK101 pKa = 10.55ARR103 pKa = 11.84AIVAIAPSDD112 pKa = 3.38QTDD115 pKa = 3.83RR116 pKa = 11.84IGPIFDD122 pKa = 4.0AAVQVAQRR130 pKa = 11.84EE131 pKa = 4.33LGVLGVVGLARR142 pKa = 11.84LAVSWHH148 pKa = 5.16GMAEE152 pKa = 4.02QVRR155 pKa = 11.84VLKK158 pKa = 10.53PGEE161 pKa = 4.2GSEE164 pKa = 4.55KK165 pKa = 10.37DD166 pKa = 3.31TT167 pKa = 4.86

Molecular weight:
17.55 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A513ZVE6|A0A513ZVE6_9VIRU Putative capsid protein OS=Fusarium redolens polymycovirus 1 OX=2546034 PE=4 SV=1
MM1 pKa = 7.8ASTTSSVQEE10 pKa = 3.77WAFSVVDD17 pKa = 3.37EE18 pKa = 4.4RR19 pKa = 11.84TRR21 pKa = 11.84VIPEE25 pKa = 3.69RR26 pKa = 11.84HH27 pKa = 4.94VVLPHH32 pKa = 5.85GEE34 pKa = 4.07RR35 pKa = 11.84AILPTPAPGSSVTTAQHH52 pKa = 5.57RR53 pKa = 11.84TPSEE57 pKa = 3.29IRR59 pKa = 11.84ADD61 pKa = 3.49HH62 pKa = 6.74AGLTEE67 pKa = 3.77AVRR70 pKa = 11.84RR71 pKa = 11.84VSLQGEE77 pKa = 4.44SRR79 pKa = 11.84RR80 pKa = 11.84TTDD83 pKa = 2.57VGLRR87 pKa = 11.84GAAHH91 pKa = 6.0SRR93 pKa = 11.84VSRR96 pKa = 11.84ASVRR100 pKa = 11.84PAATEE105 pKa = 3.88EE106 pKa = 4.28GASAVSSRR114 pKa = 11.84RR115 pKa = 11.84SEE117 pKa = 4.03NVGRR121 pKa = 11.84TGEE124 pKa = 4.35SVTTLPVYY132 pKa = 10.56RR133 pKa = 11.84SAEE136 pKa = 3.98EE137 pKa = 3.97LASSNIPQRR146 pKa = 11.84GDD148 pKa = 3.76ANVLWRR154 pKa = 11.84DD155 pKa = 3.26QTVRR159 pKa = 11.84GDD161 pKa = 3.77LEE163 pKa = 4.77SPVHH167 pKa = 6.42SGATGIPRR175 pKa = 11.84VASTATNQQEE185 pKa = 4.25GSYY188 pKa = 10.85GPEE191 pKa = 3.62NVLDD195 pKa = 4.04FQVPPTYY202 pKa = 10.79VGVPGAAVGNTGGGGSEE219 pKa = 3.73PTVVVEE225 pKa = 4.54RR226 pKa = 11.84PRR228 pKa = 11.84GRR230 pKa = 11.84RR231 pKa = 11.84SVRR234 pKa = 11.84GTRR237 pKa = 11.84RR238 pKa = 11.84ATDD241 pKa = 3.35SVGVLTSALAALPEE255 pKa = 4.71GSSFTISVAEE265 pKa = 4.05EE266 pKa = 3.78VAPDD270 pKa = 3.69GTARR274 pKa = 11.84SQASVSVTASSRR286 pKa = 11.84ASGSTSSRR294 pKa = 11.84RR295 pKa = 11.84VRR297 pKa = 11.84EE298 pKa = 3.85VGDD301 pKa = 3.39GHH303 pKa = 5.6EE304 pKa = 4.13HH305 pKa = 5.08HH306 pKa = 6.9HH307 pKa = 6.35RR308 pKa = 11.84RR309 pKa = 11.84HH310 pKa = 5.28KK311 pKa = 10.26RR312 pKa = 11.84DD313 pKa = 3.34RR314 pKa = 11.84RR315 pKa = 11.84DD316 pKa = 3.22SGVTFAEE323 pKa = 4.5WVAGRR328 pKa = 11.84RR329 pKa = 11.84KK330 pKa = 10.04

Molecular weight:
34.95 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

3294

34

771

366.0

39.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.472 ± 0.853

1.791 ± 0.676

6.132 ± 0.473

5.373 ± 0.427

3.491 ± 0.347

8.075 ± 0.939

2.398 ± 0.318

3.279 ± 0.369

3.127 ± 0.598

7.832 ± 0.864

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.004 ± 0.295

2.155 ± 0.246

5.556 ± 0.397

2.975 ± 0.288

8.409 ± 0.75

9.624 ± 0.646

5.313 ± 0.606

9.866 ± 0.765

0.88 ± 0.108

2.247 ± 0.439

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski