Mycobacterium phage Phabba

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A249XSA0|A0A249XSA0_9CAUD Esterase OS=Mycobacterium phage Phabba OX=2027899 GN=SEA_PHABBA_36 PE=4 SV=1
MM1 pKa = 7.66PALITFSRR9 pKa = 11.84DD10 pKa = 2.58WADD13 pKa = 3.38EE14 pKa = 4.22FNVDD18 pKa = 3.28GFKK21 pKa = 11.06VVDD24 pKa = 4.1TLAEE28 pKa = 3.95AADD31 pKa = 3.86YY32 pKa = 10.53KK33 pKa = 11.21SHH35 pKa = 6.73FEE37 pKa = 4.1KK38 pKa = 10.64PRR40 pKa = 11.84TYY42 pKa = 11.13YY43 pKa = 10.78FGTNEE48 pKa = 3.68GWEE51 pKa = 4.31DD52 pKa = 3.57EE53 pKa = 4.5VMVDD57 pKa = 3.66SFTITEE63 pKa = 4.17ISDD66 pKa = 3.31HH67 pKa = 6.09EE68 pKa = 4.13AAVLCKK74 pKa = 10.51LLGCAEE80 pKa = 3.99HH81 pKa = 6.53RR82 pKa = 11.84QYY84 pKa = 11.93GHH86 pKa = 6.83VPVDD90 pKa = 3.39WDD92 pKa = 4.89EE93 pKa = 4.99DD94 pKa = 3.94DD95 pKa = 5.74CDD97 pKa = 5.72DD98 pKa = 5.31DD99 pKa = 4.73VSSYY103 pKa = 11.76APP105 pKa = 3.52

Molecular weight: 11.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A249XSR4|A0A249XSR4_9CAUD Uncharacterized protein OS=Mycobacterium phage Phabba OX=2027899 GN=SEA_PHABBA_246 PE=4 SV=1
MM1 pKa = 7.46SNEE4 pKa = 3.63HH5 pKa = 5.46MTPARR10 pKa = 11.84ARR12 pKa = 11.84IEE14 pKa = 4.22TQRR17 pKa = 11.84LLDD20 pKa = 3.6AHH22 pKa = 7.01GLTGWRR28 pKa = 11.84VTFGTARR35 pKa = 11.84TRR37 pKa = 11.84NGSCSYY43 pKa = 8.93STRR46 pKa = 11.84TINLSRR52 pKa = 11.84TLMAFRR58 pKa = 11.84SYY60 pKa = 11.46EE61 pKa = 3.99EE62 pKa = 3.86TLDD65 pKa = 3.98TIRR68 pKa = 11.84HH69 pKa = 4.88EE70 pKa = 4.42VAHH73 pKa = 6.66ALTPGHH79 pKa = 5.84KK80 pKa = 9.34HH81 pKa = 5.73DD82 pKa = 4.57RR83 pKa = 11.84VWAAKK88 pKa = 9.86CRR90 pKa = 11.84EE91 pKa = 4.11LGGNGQRR98 pKa = 11.84CNGIADD104 pKa = 3.66AAKK107 pKa = 10.3RR108 pKa = 11.84EE109 pKa = 4.07QLVQTALWVGTCGHH123 pKa = 6.76GNQFPKK129 pKa = 10.28HH130 pKa = 5.34RR131 pKa = 11.84QPKK134 pKa = 9.64RR135 pKa = 11.84LVGWFCKK142 pKa = 9.73CQPGQKK148 pKa = 8.96TPVVWAKK155 pKa = 9.37TRR157 pKa = 3.29

Molecular weight: 17.74 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

244

0

244

50768

24

1256

208.1

22.99

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.569 ± 0.234

0.825 ± 0.078

6.975 ± 0.175

6.441 ± 0.179

3.132 ± 0.112

8.448 ± 0.309

2.175 ± 0.136

4.534 ± 0.128

4.367 ± 0.17

7.225 ± 0.195

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.537 ± 0.104

3.593 ± 0.113

5.584 ± 0.161

4.408 ± 0.118

6.374 ± 0.186

5.498 ± 0.169

6.301 ± 0.199

7.06 ± 0.187

1.824 ± 0.101

3.13 ± 0.115
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski