Xanthomonas phage phiLf2

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; unclassified Inoviridae

Average proteome isoelectric point is 7.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2Z4QIJ4|A0A2Z4QIJ4_9VIRU Minor coat protein OS=Xanthomonas phage phiLf2 OX=2201344 GN=phiLf2_ORF6 PE=4 SV=1
MM1 pKa = 7.78WIHH4 pKa = 5.63GRR6 pKa = 11.84AVRR9 pKa = 11.84LGRR12 pKa = 11.84RR13 pKa = 11.84QGVGCVRR20 pKa = 11.84YY21 pKa = 9.07IVVVALCLAALFFSSRR37 pKa = 11.84VSAACVQLEE46 pKa = 4.32APTSSHH52 pKa = 6.46NGDD55 pKa = 3.59WSCADD60 pKa = 3.25QGEE63 pKa = 4.4AFAKK67 pKa = 10.28VSSFPVPADD76 pKa = 3.48LAKK79 pKa = 10.62CAMKK83 pKa = 10.35AVRR86 pKa = 11.84AVASGPGFSQRR97 pKa = 11.84MTYY100 pKa = 9.86PGNTCGIGYY109 pKa = 9.63EE110 pKa = 4.07LDD112 pKa = 3.32IGTGSAQFPEE122 pKa = 4.43ASTCAKK128 pKa = 10.34RR129 pKa = 11.84PAQSGWINPTAPTPSDD145 pKa = 3.5VCNDD149 pKa = 2.93GCYY152 pKa = 8.77YY153 pKa = 10.09TYY155 pKa = 11.0SVDD158 pKa = 3.29PGNPKK163 pKa = 10.27GYY165 pKa = 10.33SYY167 pKa = 10.46TPSGATCTTDD177 pKa = 4.31DD178 pKa = 4.42AAPPIDD184 pKa = 4.94DD185 pKa = 5.12GGDD188 pKa = 3.49GSDD191 pKa = 4.1GDD193 pKa = 4.09GGGDD197 pKa = 3.3GGGDD201 pKa = 3.3GGGDD205 pKa = 3.3GGGDD209 pKa = 3.3GGGDD213 pKa = 3.3GGGDD217 pKa = 3.3GGGDD221 pKa = 3.3GGGDD225 pKa = 3.3GGGDD229 pKa = 3.25GGGDD233 pKa = 3.52GDD235 pKa = 5.14GDD237 pKa = 4.05GDD239 pKa = 4.77GDD241 pKa = 4.51GDD243 pKa = 4.1TPGDD247 pKa = 3.86GDD249 pKa = 3.9GTTPGDD255 pKa = 3.76GEE257 pKa = 4.46GGEE260 pKa = 4.26GAPMSEE266 pKa = 4.89LYY268 pKa = 10.33KK269 pKa = 10.79KK270 pKa = 10.4SGKK273 pKa = 7.4TVEE276 pKa = 4.37SVLSKK281 pKa = 11.02FNTQVRR287 pKa = 11.84ATPMVGGITDD297 pKa = 4.47FMTVPSGGSCPVFSLGASKK316 pKa = 8.92WWNAMTINFHH326 pKa = 6.93CGGDD330 pKa = 3.5FLAFLRR336 pKa = 11.84AAGWVILAIAAYY348 pKa = 8.93AALRR352 pKa = 11.84IAVTT356 pKa = 3.72

Molecular weight: 35.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2Z4QH79|A0A2Z4QH79_9VIRU Minor coat protein OS=Xanthomonas phage phiLf2 OX=2201344 GN=phiLf2_ORF7 PE=4 SV=1
MM1 pKa = 7.58ARR3 pKa = 11.84WCPPANRR10 pKa = 11.84LASAAATCSSVVANTACMRR29 pKa = 11.84WCKK32 pKa = 10.27KK33 pKa = 10.12RR34 pKa = 11.84SQCPARR40 pKa = 11.84RR41 pKa = 11.84TPP43 pKa = 3.36

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

1989

38

440

180.8

19.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.167 ± 0.804

1.961 ± 0.512

6.285 ± 0.799

3.922 ± 0.442

3.318 ± 0.316

11.111 ± 1.66

1.508 ± 0.299

3.922 ± 0.319

4.827 ± 0.565

7.541 ± 0.76

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.665 ± 0.462

2.363 ± 0.303

5.078 ± 0.479

3.067 ± 0.4

6.687 ± 0.836

5.681 ± 0.442

5.38 ± 0.255

7.34 ± 0.604

2.413 ± 0.309

2.765 ± 0.352
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski