Amino acid dipepetide frequency for Natronomonas salsuginis

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.095AlaAla: 13.095 ± 0.224
0.741AlaCys: 0.741 ± 0.033
9.102AlaAsp: 9.102 ± 0.127
8.925AlaGlu: 8.925 ± 0.141
4.054AlaPhe: 4.054 ± 0.078
9.125AlaGly: 9.125 ± 0.122
1.876AlaHis: 1.876 ± 0.051
5.778AlaIle: 5.778 ± 0.093
1.976AlaLys: 1.976 ± 0.059
10.014AlaLeu: 10.014 ± 0.175
2.07AlaMet: 2.07 ± 0.063
2.438AlaAsn: 2.438 ± 0.071
3.82AlaPro: 3.82 ± 0.072
1.997AlaGln: 1.997 ± 0.047
6.149AlaArg: 6.149 ± 0.095
5.451AlaSer: 5.451 ± 0.161
6.622AlaThr: 6.622 ± 0.167
10.025AlaVal: 10.025 ± 0.132
1.026AlaTrp: 1.026 ± 0.038
2.825AlaTyr: 2.825 ± 0.075
0.0AlaXaa: 0.0 ± 0.0
Cys
0.643CysAla: 0.643 ± 0.035
0.092CysCys: 0.092 ± 0.012
0.566CysAsp: 0.566 ± 0.028
0.575CysGlu: 0.575 ± 0.027
0.217CysPhe: 0.217 ± 0.016
0.91CysGly: 0.91 ± 0.039
0.184CysHis: 0.184 ± 0.017
0.28CysIle: 0.28 ± 0.017
0.126CysLys: 0.126 ± 0.013
0.587CysLeu: 0.587 ± 0.031
0.11CysMet: 0.11 ± 0.012
0.211CysAsn: 0.211 ± 0.018
0.549CysPro: 0.549 ± 0.028
0.143CysGln: 0.143 ± 0.013
0.489CysArg: 0.489 ± 0.024
0.437CysSer: 0.437 ± 0.023
0.424CysThr: 0.424 ± 0.026
0.571CysVal: 0.571 ± 0.024
0.092CysTrp: 0.092 ± 0.011
0.188CysTyr: 0.188 ± 0.014
0.0CysXaa: 0.0 ± 0.0
Asp
9.985AspAla: 9.985 ± 0.16
0.616AspCys: 0.616 ± 0.029
7.491AspAsp: 7.491 ± 0.121
7.744AspGlu: 7.744 ± 0.125
1.969AspPhe: 1.969 ± 0.054
8.296AspGly: 8.296 ± 0.123
1.672AspHis: 1.672 ± 0.05
3.542AspIle: 3.542 ± 0.078
1.014AspLys: 1.014 ± 0.041
6.457AspLeu: 6.457 ± 0.104
1.183AspMet: 1.183 ± 0.043
1.285AspAsn: 1.285 ± 0.053
4.57AspPro: 4.57 ± 0.1
1.349AspGln: 1.349 ± 0.043
6.735AspArg: 6.735 ± 0.107
3.872AspSer: 3.872 ± 0.071
3.999AspThr: 3.999 ± 0.081
7.26AspVal: 7.26 ± 0.106
0.755AspTrp: 0.755 ± 0.033
1.638AspTyr: 1.638 ± 0.05
0.0AspXaa: 0.0 ± 0.0
Glu
8.975GluAla: 8.975 ± 0.14
0.566GluCys: 0.566 ± 0.033
4.88GluAsp: 4.88 ± 0.083
6.384GluGlu: 6.384 ± 0.127
3.024GluPhe: 3.024 ± 0.08
5.331GluGly: 5.331 ± 0.089
2.03GluHis: 2.03 ± 0.055
4.701GluIle: 4.701 ± 0.094
1.837GluLys: 1.837 ± 0.056
8.464GluLeu: 8.464 ± 0.131
1.9GluMet: 1.9 ± 0.056
2.338GluAsn: 2.338 ± 0.056
3.988GluPro: 3.988 ± 0.093
2.334GluGln: 2.334 ± 0.062
7.619GluArg: 7.619 ± 0.149
5.326GluSer: 5.326 ± 0.093
6.877GluThr: 6.877 ± 0.105
5.251GluVal: 5.251 ± 0.112
1.156GluTrp: 1.156 ± 0.041
2.749GluTyr: 2.749 ± 0.066
0.0GluXaa: 0.0 ± 0.0
Phe
3.548PheAla: 3.548 ± 0.083
0.3PheCys: 0.3 ± 0.019
3.44PheAsp: 3.44 ± 0.075
3.623PheGlu: 3.623 ± 0.084
1.038PhePhe: 1.038 ± 0.042
3.493PheGly: 3.493 ± 0.074
0.622PheHis: 0.622 ± 0.027
1.211PheIle: 1.211 ± 0.046
0.546PheLys: 0.546 ± 0.027
2.654PheLeu: 2.654 ± 0.069
0.505PheMet: 0.505 ± 0.028
0.73PheAsn: 0.73 ± 0.029
1.271PhePro: 1.271 ± 0.044
0.708PheGln: 0.708 ± 0.03
1.77PheArg: 1.77 ± 0.049
1.767PheSer: 1.767 ± 0.043
1.798PheThr: 1.798 ± 0.05
3.317PheVal: 3.317 ± 0.084
0.332PheTrp: 0.332 ± 0.022
0.866PheTyr: 0.866 ± 0.032
0.0PheXaa: 0.0 ± 0.0
Gly
8.004GlyAla: 8.004 ± 0.115
0.709GlyCys: 0.709 ± 0.03
6.723GlyAsp: 6.723 ± 0.116
7.253GlyGlu: 7.253 ± 0.101
3.277GlyPhe: 3.277 ± 0.066
7.814GlyGly: 7.814 ± 0.231
1.755GlyHis: 1.755 ± 0.048
4.748GlyIle: 4.748 ± 0.084
1.932GlyLys: 1.932 ± 0.05
7.467GlyLeu: 7.467 ± 0.155
1.855GlyMet: 1.855 ± 0.05
2.17GlyAsn: 2.17 ± 0.123
3.203GlyPro: 3.203 ± 0.064
1.976GlyGln: 1.976 ± 0.063
5.283GlyArg: 5.283 ± 0.093
5.402GlySer: 5.402 ± 0.162
5.942GlyThr: 5.942 ± 0.176
7.577GlyVal: 7.577 ± 0.112
1.102GlyTrp: 1.102 ± 0.036
2.773GlyTyr: 2.773 ± 0.069
0.0GlyXaa: 0.0 ± 0.0
His
2.047HisAla: 2.047 ± 0.052
0.192HisCys: 0.192 ± 0.013
1.75HisAsp: 1.75 ± 0.053
1.712HisGlu: 1.712 ± 0.049
0.574HisPhe: 0.574 ± 0.029
2.041HisGly: 2.041 ± 0.053
0.529HisHis: 0.529 ± 0.028
0.805HisIle: 0.805 ± 0.037
0.316HisLys: 0.316 ± 0.022
1.611HisLeu: 1.611 ± 0.049
0.292HisMet: 0.292 ± 0.02
0.483HisAsn: 0.483 ± 0.024
1.267HisPro: 1.267 ± 0.043
0.41HisGln: 0.41 ± 0.023
1.348HisArg: 1.348 ± 0.044
0.909HisSer: 0.909 ± 0.037
1.119HisThr: 1.119 ± 0.038
1.813HisVal: 1.813 ± 0.056
0.19HisTrp: 0.19 ± 0.014
0.584HisTyr: 0.584 ± 0.031
0.0HisXaa: 0.0 ± 0.0
Ile
5.497IleAla: 5.497 ± 0.089
0.311IleCys: 0.311 ± 0.022
4.898IleAsp: 4.898 ± 0.092
5.454IleGlu: 5.454 ± 0.1
1.088IlePhe: 1.088 ± 0.041
4.685IleGly: 4.685 ± 0.095
0.972IleHis: 0.972 ± 0.037
1.676IleIle: 1.676 ± 0.059
0.95IleLys: 0.95 ± 0.038
3.181IleLeu: 3.181 ± 0.072
0.589IleMet: 0.589 ± 0.031
1.127IleAsn: 1.127 ± 0.05
2.232IlePro: 2.232 ± 0.055
1.114IleGln: 1.114 ± 0.039
2.84IleArg: 2.84 ± 0.066
2.384IleSer: 2.384 ± 0.066
2.746IleThr: 2.746 ± 0.085
4.475IleVal: 4.475 ± 0.097
0.359IleTrp: 0.359 ± 0.023
0.983IleTyr: 0.983 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
1.911LysAla: 1.911 ± 0.059
0.104LysCys: 0.104 ± 0.011
1.102LysAsp: 1.102 ± 0.04
1.383LysGlu: 1.383 ± 0.05
0.543LysPhe: 0.543 ± 0.028
1.349LysGly: 1.349 ± 0.046
0.557LysHis: 0.557 ± 0.025
0.944LysIle: 0.944 ± 0.04
0.594LysLys: 0.594 ± 0.034
1.802LysLeu: 1.802 ± 0.051
0.391LysMet: 0.391 ± 0.024
0.588LysAsn: 0.588 ± 0.027
1.001LysPro: 1.001 ± 0.041
0.799LysGln: 0.799 ± 0.038
1.934LysArg: 1.934 ± 0.056
1.225LysSer: 1.225 ± 0.047
1.448LysThr: 1.448 ± 0.045
1.237LysVal: 1.237 ± 0.04
0.234LysTrp: 0.234 ± 0.017
0.57LysTyr: 0.57 ± 0.026
0.0LysXaa: 0.0 ± 0.0
Leu
10.17LeuAla: 10.17 ± 0.164
0.668LeuCys: 0.668 ± 0.028
7.845LeuAsp: 7.845 ± 0.126
7.033LeuGlu: 7.033 ± 0.13
3.181LeuPhe: 3.181 ± 0.076
7.617LeuGly: 7.617 ± 0.124
1.487LeuHis: 1.487 ± 0.046
3.426LeuIle: 3.426 ± 0.078
1.738LeuLys: 1.738 ± 0.057
8.245LeuLeu: 8.245 ± 0.173
1.332LeuMet: 1.332 ± 0.047
1.868LeuAsn: 1.868 ± 0.046
3.97LeuPro: 3.97 ± 0.081
1.888LeuGln: 1.888 ± 0.054
5.5LeuArg: 5.5 ± 0.103
5.715LeuSer: 5.715 ± 0.087
5.026LeuThr: 5.026 ± 0.087
8.02LeuVal: 8.02 ± 0.12
0.836LeuTrp: 0.836 ± 0.036
2.209LeuTyr: 2.209 ± 0.065
0.0LeuXaa: 0.0 ± 0.0
Met
1.878MetAla: 1.878 ± 0.05
0.142MetCys: 0.142 ± 0.014
1.303MetAsp: 1.303 ± 0.04
1.096MetGlu: 1.096 ± 0.037
0.56MetPhe: 0.56 ± 0.029
1.54MetGly: 1.54 ± 0.058
0.367MetHis: 0.367 ± 0.021
0.773MetIle: 0.773 ± 0.033
0.492MetLys: 0.492 ± 0.026
1.55MetLeu: 1.55 ± 0.049
0.35MetMet: 0.35 ± 0.024
0.607MetAsn: 0.607 ± 0.03
0.787MetPro: 0.787 ± 0.029
0.448MetGln: 0.448 ± 0.025
1.186MetArg: 1.186 ± 0.041
1.519MetSer: 1.519 ± 0.044
1.479MetThr: 1.479 ± 0.047
1.307MetVal: 1.307 ± 0.046
0.148MetTrp: 0.148 ± 0.012
0.439MetTyr: 0.439 ± 0.024
0.0MetXaa: 0.0 ± 0.0
Asn
2.871AsnAla: 2.871 ± 0.109
0.25AsnCys: 0.25 ± 0.016
1.723AsnAsp: 1.723 ± 0.06
1.863AsnGlu: 1.863 ± 0.056
0.659AsnPhe: 0.659 ± 0.028
2.305AsnGly: 2.305 ± 0.103
0.53AsnHis: 0.53 ± 0.031
1.006AsnIle: 1.006 ± 0.036
0.507AsnLys: 0.507 ± 0.03
1.986AsnLeu: 1.986 ± 0.048
0.422AsnMet: 0.422 ± 0.023
0.652AsnAsn: 0.652 ± 0.046
1.516AsnPro: 1.516 ± 0.046
0.61AsnGln: 0.61 ± 0.035
1.688AsnArg: 1.688 ± 0.042
1.021AsnSer: 1.021 ± 0.062
1.348AsnThr: 1.348 ± 0.061
2.464AsnVal: 2.464 ± 0.089
0.271AsnTrp: 0.271 ± 0.017
0.673AsnTyr: 0.673 ± 0.032
0.0AsnXaa: 0.0 ± 0.0
Pro
4.292ProAla: 4.292 ± 0.091
0.269ProCys: 0.269 ± 0.017
4.576ProAsp: 4.576 ± 0.088
4.632ProGlu: 4.632 ± 0.087
1.63ProPhe: 1.63 ± 0.046
3.714ProGly: 3.714 ± 0.072
0.865ProHis: 0.865 ± 0.034
2.435ProIle: 2.435 ± 0.055
1.014ProLys: 1.014 ± 0.034
3.541ProLeu: 3.541 ± 0.071
0.891ProMet: 0.891 ± 0.039
1.228ProAsn: 1.228 ± 0.039
2.164ProPro: 2.164 ± 0.059
0.886ProGln: 0.886 ± 0.031
2.262ProArg: 2.262 ± 0.056
2.565ProSer: 2.565 ± 0.055
3.29ProThr: 3.29 ± 0.068
3.894ProVal: 3.894 ± 0.071
0.461ProTrp: 0.461 ± 0.025
1.157ProTyr: 1.157 ± 0.036
0.0ProXaa: 0.0 ± 0.0
Gln
2.107GlnAla: 2.107 ± 0.063
0.143GlnCys: 0.143 ± 0.014
1.133GlnAsp: 1.133 ± 0.039
1.421GlnGlu: 1.421 ± 0.046
0.976GlnPhe: 0.976 ± 0.039
1.467GlnGly: 1.467 ± 0.044
0.48GlnHis: 0.48 ± 0.024
1.137GlnIle: 1.137 ± 0.041
0.617GlnLys: 0.617 ± 0.031
2.323GlnLeu: 2.323 ± 0.055
0.525GlnMet: 0.525 ± 0.024
0.687GlnAsn: 0.687 ± 0.038
1.026GlnPro: 1.026 ± 0.033
0.914GlnGln: 0.914 ± 0.043
1.974GlnArg: 1.974 ± 0.054
1.456GlnSer: 1.456 ± 0.057
1.559GlnThr: 1.559 ± 0.049
1.528GlnVal: 1.528 ± 0.048
0.282GlnTrp: 0.282 ± 0.02
0.805GlnTyr: 0.805 ± 0.034
0.0GlnXaa: 0.0 ± 0.0
Arg
6.581ArgAla: 6.581 ± 0.113
0.52ArgCys: 0.52 ± 0.028
4.676ArgAsp: 4.676 ± 0.091
6.279ArgGlu: 6.279 ± 0.123
2.472ArgPhe: 2.472 ± 0.066
4.701ArgGly: 4.701 ± 0.089
1.278ArgHis: 1.278 ± 0.045
3.577ArgIle: 3.577 ± 0.068
1.467ArgLys: 1.467 ± 0.045
6.39ArgLeu: 6.39 ± 0.1
1.422ArgMet: 1.422 ± 0.045
1.844ArgAsn: 1.844 ± 0.045
2.672ArgPro: 2.672 ± 0.078
1.727ArgGln: 1.727 ± 0.049
5.319ArgArg: 5.319 ± 0.12
3.96ArgSer: 3.96 ± 0.074
4.366ArgThr: 4.366 ± 0.075
5.215ArgVal: 5.215 ± 0.107
0.756ArgTrp: 0.756 ± 0.035
2.052ArgTyr: 2.052 ± 0.053
0.0ArgXaa: 0.0 ± 0.0
Ser
5.486SerAla: 5.486 ± 0.141
0.333SerCys: 0.333 ± 0.022
4.308SerAsp: 4.308 ± 0.088
4.446SerGlu: 4.446 ± 0.085
1.905SerPhe: 1.905 ± 0.053
5.599SerGly: 5.599 ± 0.188
1.024SerHis: 1.024 ± 0.034
3.076SerIle: 3.076 ± 0.064
1.387SerLys: 1.387 ± 0.043
4.574SerLeu: 4.574 ± 0.078
1.188SerMet: 1.188 ± 0.042
1.536SerAsn: 1.536 ± 0.084
2.608SerPro: 2.608 ± 0.059
1.273SerGln: 1.273 ± 0.042
3.273SerArg: 3.273 ± 0.074
3.107SerSer: 3.107 ± 0.112
3.594SerThr: 3.594 ± 0.113
5.203SerVal: 5.203 ± 0.086
0.546SerTrp: 0.546 ± 0.03
1.425SerTyr: 1.425 ± 0.055
0.0SerXaa: 0.0 ± 0.0
Thr
6.984ThrAla: 6.984 ± 0.14
0.375ThrCys: 0.375 ± 0.024
5.46ThrAsp: 5.46 ± 0.106
4.74ThrGlu: 4.74 ± 0.081
2.188ThrPhe: 2.188 ± 0.056
5.691ThrGly: 5.691 ± 0.157
1.31ThrHis: 1.31 ± 0.036
3.407ThrIle: 3.407 ± 0.08
1.146ThrLys: 1.146 ± 0.036
5.642ThrLeu: 5.642 ± 0.082
1.019ThrMet: 1.019 ± 0.035
1.55ThrAsn: 1.55 ± 0.077
3.355ThrPro: 3.355 ± 0.068
1.25ThrGln: 1.25 ± 0.038
3.426ThrArg: 3.426 ± 0.076
2.845ThrSer: 2.845 ± 0.114
4.124ThrThr: 4.124 ± 0.138
7.019ThrVal: 7.019 ± 0.17
0.569ThrTrp: 0.569 ± 0.027
1.788ThrTyr: 1.788 ± 0.062
0.0ThrXaa: 0.0 ± 0.0
Val
9.369ValAla: 9.369 ± 0.147
0.722ValCys: 0.722 ± 0.031
7.189ValAsp: 7.189 ± 0.113
8.067ValGlu: 8.067 ± 0.137
2.913ValPhe: 2.913 ± 0.067
8.001ValGly: 8.001 ± 0.192
1.614ValHis: 1.614 ± 0.049
3.642ValIle: 3.642 ± 0.081
1.349ValLys: 1.349 ± 0.043
7.621ValLeu: 7.621 ± 0.125
1.278ValMet: 1.278 ± 0.039
2.005ValAsn: 2.005 ± 0.075
4.118ValPro: 4.118 ± 0.081
1.758ValGln: 1.758 ± 0.049
5.571ValArg: 5.571 ± 0.088
5.214ValSer: 5.214 ± 0.12
5.933ValThr: 5.933 ± 0.177
8.41ValVal: 8.41 ± 0.126
0.766ValTrp: 0.766 ± 0.032
2.143ValTyr: 2.143 ± 0.052
0.0ValXaa: 0.0 ± 0.0
Trp
0.837TrpAla: 0.837 ± 0.03
0.086TrpCys: 0.086 ± 0.012
0.719TrpAsp: 0.719 ± 0.035
0.732TrpGlu: 0.732 ± 0.033
0.425TrpPhe: 0.425 ± 0.021
0.805TrpGly: 0.805 ± 0.034
0.215TrpHis: 0.215 ± 0.018
0.546TrpIle: 0.546 ± 0.025
0.271TrpLys: 0.271 ± 0.019
1.131TrpLeu: 1.131 ± 0.039
0.235TrpMet: 0.235 ± 0.018
0.338TrpAsn: 0.338 ± 0.025
0.407TrpPro: 0.407 ± 0.024
0.296TrpGln: 0.296 ± 0.019
0.842TrpArg: 0.842 ± 0.036
0.534TrpSer: 0.534 ± 0.028
0.647TrpThr: 0.647 ± 0.03
0.755TrpVal: 0.755 ± 0.031
0.177TrpTrp: 0.177 ± 0.016
0.359TrpTyr: 0.359 ± 0.023
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.828TyrAla: 2.828 ± 0.062
0.252TyrCys: 0.252 ± 0.017
2.551TyrAsp: 2.551 ± 0.067
2.372TyrGlu: 2.372 ± 0.058
0.846TyrPhe: 0.846 ± 0.033
2.482TyrGly: 2.482 ± 0.058
0.624TyrHis: 0.624 ± 0.029
0.852TyrIle: 0.852 ± 0.036
0.436TyrLys: 0.436 ± 0.023
2.535TyrLeu: 2.535 ± 0.059
0.413TyrMet: 0.413 ± 0.023
0.682TyrAsn: 0.682 ± 0.029
1.24TyrPro: 1.24 ± 0.037
0.68TyrGln: 0.68 ± 0.03
2.067TyrArg: 2.067 ± 0.051
1.181TyrSer: 1.181 ± 0.041
1.548TyrThr: 1.548 ± 0.049
2.341TyrVal: 2.341 ± 0.058
0.3TyrTrp: 0.3 ± 0.023
0.824TyrTyr: 0.824 ± 0.041
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2818 proteins (826435 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski