Oryza brachyantha

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; Liliopsida; Petrosaviidae; commelinids; Poales; Poaceae; BOP clade; Oryzoideae; Oryzeae; Oryzinae; Oryza

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31989 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J3L809|J3L809_ORYBR Apple domain-containing protein OS=Oryza brachyantha OX=4533 PE=4 SV=1
HHH2 pKa = 7.85FPGPVRR8 pKa = 11.84PDDD11 pKa = 3.19VLEEE15 pKa = 4.15MVGNPANPGQAMPDDD30 pKa = 3.34PAAADDD36 pKa = 3.78STIDDD41 pKa = 5.34AMLYYY46 pKa = 10.66KKK48 pKa = 10.06LNHHH52 pKa = 7.28PTDDD56 pKa = 3.61VVGGAVTPEEE66 pKa = 4.27AGHHH70 pKa = 6.08DDD72 pKa = 3.84DD73 pKa = 6.45FDDD76 pKa = 3.72FSASSDDD83 pKa = 3.52SPGILAPAQFDDD95 pKa = 3.67SQDDD99 pKa = 3.42FGEEE103 pKa = 4.24SSPASGNDDD112 pKa = 3.23TSATTATTTMLCTDDD127 pKa = 4.41SVQAALGEEE136 pKa = 4.69NFAMDDD142 pKa = 3.58SCFDDD147 pKa = 3.94LGLPTDDD154 pKa = 3.53GGGGSPSSGGSIVPSLSTWEEE175 pKa = 4.01EE176 pKa = 3.7KKK178 pKa = 10.66YY179 pKa = 10.88DD180 pKa = 4.06LDDD183 pKa = 3.76FPDDD187 pKa = 3.51DD188 pKa = 4.48MSLHHH193 pKa = 6.95EE194 pKa = 4.8MISAPDDD201 pKa = 3.84HH202 pKa = 6.73DD203 pKa = 4.46SVDDD207 pKa = 3.78QGLEEE212 pKa = 4.09LYYY215 pKa = 9.83P

Molecular weight: 22.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J3NBH6|J3NBH6_ORYBR Uncharacterized protein OS=Oryza brachyantha OX=4533 PE=4 SV=1
MM1 pKa = 7.61GGPMLSAASRR11 pKa = 11.84PAMSPPADD19 pKa = 4.32APPSLATRR27 pKa = 11.84SPASLSARR35 pKa = 11.84PSSAAGPVRR44 pKa = 11.84WRR46 pKa = 11.84RR47 pKa = 11.84RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84RR51 pKa = 11.84RR52 pKa = 11.84QPRR55 pKa = 11.84RR56 pKa = 11.84GSGARR61 pKa = 11.84TRR63 pKa = 11.84RR64 pKa = 11.84RR65 pKa = 11.84TPAPPSRR72 pKa = 11.84ASRR75 pKa = 11.84RR76 pKa = 11.84PSSRR80 pKa = 11.84RR81 pKa = 11.84RR82 pKa = 11.84RR83 pKa = 11.84RR84 pKa = 11.84GGSS87 pKa = 2.96

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

31989

0

31989

11295499

28

5111

353.1

38.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.617 ± 0.02

1.909 ± 0.006

5.286 ± 0.012

5.936 ± 0.016

3.664 ± 0.008

7.2 ± 0.018

2.47 ± 0.007

4.452 ± 0.012

4.949 ± 0.015

9.38 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.451 ± 0.007

3.545 ± 0.01

5.297 ± 0.016

3.44 ± 0.011

6.188 ± 0.015

8.173 ± 0.02

4.739 ± 0.008

6.688 ± 0.011

1.281 ± 0.005

2.58 ± 0.008
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski