Amino acid dipepetide frequency for Aspergillus niger (strain ATCC 1015 / CBS 113.46 / FGSC A1144 / LSHB Ac4 / NCTC 3858a / NRRL 328 / USDA 3528.7)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.743AlaAla: 8.743 ± 0.056
1.138AlaCys: 1.138 ± 0.016
4.197AlaAsp: 4.197 ± 0.031
4.967AlaGlu: 4.967 ± 0.04
3.206AlaPhe: 3.206 ± 0.027
5.801AlaGly: 5.801 ± 0.036
1.807AlaHis: 1.807 ± 0.018
4.362AlaIle: 4.362 ± 0.035
3.688AlaLys: 3.688 ± 0.03
7.902AlaLeu: 7.902 ± 0.043
2.006AlaMet: 2.006 ± 0.018
2.879AlaAsn: 2.879 ± 0.022
4.538AlaPro: 4.538 ± 0.042
3.286AlaGln: 3.286 ± 0.029
4.825AlaArg: 4.825 ± 0.036
7.157AlaSer: 7.157 ± 0.045
5.246AlaThr: 5.246 ± 0.039
5.515AlaVal: 5.515 ± 0.036
1.215AlaTrp: 1.215 ± 0.019
2.296AlaTyr: 2.296 ± 0.021
0.0AlaXaa: 0.0 ± 0.0
Cys
0.988CysAla: 0.988 ± 0.014
0.251CysCys: 0.251 ± 0.007
0.697CysAsp: 0.697 ± 0.013
0.62CysGlu: 0.62 ± 0.012
0.569CysPhe: 0.569 ± 0.011
0.941CysGly: 0.941 ± 0.015
0.365CysHis: 0.365 ± 0.01
0.755CysIle: 0.755 ± 0.013
0.478CysLys: 0.478 ± 0.008
1.404CysLeu: 1.404 ± 0.019
0.291CysMet: 0.291 ± 0.008
0.434CysAsn: 0.434 ± 0.01
0.664CysPro: 0.664 ± 0.012
0.477CysGln: 0.477 ± 0.01
0.807CysArg: 0.807 ± 0.014
0.979CysSer: 0.979 ± 0.014
0.725CysThr: 0.725 ± 0.013
0.87CysVal: 0.87 ± 0.014
0.22CysTrp: 0.22 ± 0.006
0.374CysTyr: 0.374 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
4.564AspAla: 4.564 ± 0.03
0.623AspCys: 0.623 ± 0.011
3.921AspAsp: 3.921 ± 0.04
4.22AspGlu: 4.22 ± 0.042
2.132AspPhe: 2.132 ± 0.023
3.931AspGly: 3.931 ± 0.032
1.258AspHis: 1.258 ± 0.017
3.172AspIle: 3.172 ± 0.027
2.186AspLys: 2.186 ± 0.023
5.19AspLeu: 5.19 ± 0.038
1.257AspMet: 1.257 ± 0.017
1.861AspAsn: 1.861 ± 0.019
3.337AspPro: 3.337 ± 0.027
1.892AspGln: 1.892 ± 0.019
3.09AspArg: 3.09 ± 0.024
3.973AspSer: 3.973 ± 0.03
3.002AspThr: 3.002 ± 0.027
3.712AspVal: 3.712 ± 0.026
0.92AspTrp: 0.92 ± 0.014
1.733AspTyr: 1.733 ± 0.017
0.0AspXaa: 0.0 ± 0.0
Glu
5.231GluAla: 5.231 ± 0.038
0.65GluCys: 0.65 ± 0.012
4.007GluAsp: 4.007 ± 0.037
5.422GluGlu: 5.422 ± 0.056
1.925GluPhe: 1.925 ± 0.018
3.827GluGly: 3.827 ± 0.029
1.381GluHis: 1.381 ± 0.018
2.985GluIle: 2.985 ± 0.024
3.43GluLys: 3.43 ± 0.033
5.146GluLeu: 5.146 ± 0.034
1.443GluMet: 1.443 ± 0.017
2.185GluAsn: 2.185 ± 0.02
2.754GluPro: 2.754 ± 0.036
2.507GluGln: 2.507 ± 0.026
3.824GluArg: 3.824 ± 0.034
4.263GluSer: 4.263 ± 0.031
3.487GluThr: 3.487 ± 0.028
3.639GluVal: 3.639 ± 0.031
0.915GluTrp: 0.915 ± 0.015
1.784GluTyr: 1.784 ± 0.017
0.0GluXaa: 0.0 ± 0.0
Phe
3.032PheAla: 3.032 ± 0.027
0.607PheCys: 0.607 ± 0.012
2.249PheAsp: 2.249 ± 0.021
2.09PheGlu: 2.09 ± 0.023
1.687PhePhe: 1.687 ± 0.024
2.861PheGly: 2.861 ± 0.026
0.969PheHis: 0.969 ± 0.014
1.899PheIle: 1.899 ± 0.023
1.364PheLys: 1.364 ± 0.017
3.7PheLeu: 3.7 ± 0.031
0.788PheMet: 0.788 ± 0.013
1.407PheAsn: 1.407 ± 0.016
2.025PhePro: 2.025 ± 0.02
1.418PheGln: 1.418 ± 0.017
2.077PheArg: 2.077 ± 0.021
3.042PheSer: 3.042 ± 0.029
2.226PheThr: 2.226 ± 0.022
2.447PheVal: 2.447 ± 0.026
0.681PheTrp: 0.681 ± 0.011
1.189PheTyr: 1.189 ± 0.016
0.0PheXaa: 0.0 ± 0.0
Gly
5.282GlyAla: 5.282 ± 0.039
0.93GlyCys: 0.93 ± 0.012
3.634GlyAsp: 3.634 ± 0.031
3.652GlyGlu: 3.652 ± 0.029
2.854GlyPhe: 2.854 ± 0.028
5.515GlyGly: 5.515 ± 0.049
1.687GlyHis: 1.687 ± 0.02
3.649GlyIle: 3.649 ± 0.029
3.231GlyLys: 3.231 ± 0.024
6.312GlyLeu: 6.312 ± 0.036
1.647GlyMet: 1.647 ± 0.021
2.434GlyAsn: 2.434 ± 0.024
3.329GlyPro: 3.329 ± 0.031
2.549GlyGln: 2.549 ± 0.024
4.07GlyArg: 4.07 ± 0.03
5.663GlySer: 5.663 ± 0.038
3.978GlyThr: 3.978 ± 0.032
4.623GlyVal: 4.623 ± 0.036
1.232GlyTrp: 1.232 ± 0.016
2.284GlyTyr: 2.284 ± 0.026
0.0GlyXaa: 0.0 ± 0.0
His
1.884HisAla: 1.884 ± 0.021
0.351HisCys: 0.351 ± 0.008
1.313HisAsp: 1.313 ± 0.014
1.318HisGlu: 1.318 ± 0.016
0.931HisPhe: 0.931 ± 0.013
1.714HisGly: 1.714 ± 0.02
0.903HisHis: 0.903 ± 0.017
1.256HisIle: 1.256 ± 0.015
0.835HisLys: 0.835 ± 0.012
2.383HisLeu: 2.383 ± 0.02
0.5HisMet: 0.5 ± 0.011
0.861HisAsn: 0.861 ± 0.013
1.771HisPro: 1.771 ± 0.022
0.977HisGln: 0.977 ± 0.015
1.557HisArg: 1.557 ± 0.02
1.821HisSer: 1.821 ± 0.021
1.34HisThr: 1.34 ± 0.018
1.452HisVal: 1.452 ± 0.019
0.367HisTrp: 0.367 ± 0.009
0.756HisTyr: 0.756 ± 0.011
0.0HisXaa: 0.0 ± 0.0
Ile
4.249IleAla: 4.249 ± 0.035
0.83IleCys: 0.83 ± 0.014
2.838IleAsp: 2.838 ± 0.024
2.798IleGlu: 2.798 ± 0.026
2.098IlePhe: 2.098 ± 0.023
3.329IleGly: 3.329 ± 0.032
1.283IleHis: 1.283 ± 0.019
2.669IleIle: 2.669 ± 0.026
2.01IleLys: 2.01 ± 0.022
4.874IleLeu: 4.874 ± 0.038
1.043IleMet: 1.043 ± 0.016
1.816IleAsn: 1.816 ± 0.021
3.197IlePro: 3.197 ± 0.026
1.955IleGln: 1.955 ± 0.02
2.876IleArg: 2.876 ± 0.026
3.941IleSer: 3.941 ± 0.031
2.932IleThr: 2.932 ± 0.027
3.284IleVal: 3.284 ± 0.029
0.757IleTrp: 0.757 ± 0.014
1.555IleTyr: 1.555 ± 0.017
0.0IleXaa: 0.0 ± 0.0
Lys
3.842LysAla: 3.842 ± 0.033
0.47LysCys: 0.47 ± 0.009
2.53LysAsp: 2.53 ± 0.026
3.165LysGlu: 3.165 ± 0.032
1.328LysPhe: 1.328 ± 0.015
2.785LysGly: 2.785 ± 0.026
1.052LysHis: 1.052 ± 0.014
2.124LysIle: 2.124 ± 0.023
2.818LysLys: 2.818 ± 0.037
3.849LysLeu: 3.849 ± 0.031
0.89LysMet: 0.89 ± 0.013
1.582LysAsn: 1.582 ± 0.018
2.526LysPro: 2.526 ± 0.025
1.751LysGln: 1.751 ± 0.022
3.117LysArg: 3.117 ± 0.029
3.168LysSer: 3.168 ± 0.027
2.516LysThr: 2.516 ± 0.027
2.701LysVal: 2.701 ± 0.025
0.645LysTrp: 0.645 ± 0.011
1.357LysTyr: 1.357 ± 0.017
0.0LysXaa: 0.0 ± 0.0
Leu
8.005LeuAla: 8.005 ± 0.046
1.331LeuCys: 1.331 ± 0.017
5.254LeuAsp: 5.254 ± 0.032
5.53LeuGlu: 5.53 ± 0.041
3.534LeuPhe: 3.534 ± 0.032
6.218LeuGly: 6.218 ± 0.043
2.353LeuHis: 2.353 ± 0.023
4.214LeuIle: 4.214 ± 0.035
3.951LeuLys: 3.951 ± 0.034
8.911LeuLeu: 8.911 ± 0.053
1.91LeuMet: 1.91 ± 0.019
3.232LeuAsn: 3.232 ± 0.026
5.612LeuPro: 5.612 ± 0.04
3.993LeuGln: 3.993 ± 0.032
5.909LeuArg: 5.909 ± 0.041
7.67LeuSer: 7.67 ± 0.041
5.059LeuThr: 5.059 ± 0.032
5.702LeuVal: 5.702 ± 0.041
1.282LeuTrp: 1.282 ± 0.016
2.626LeuTyr: 2.626 ± 0.025
0.0LeuXaa: 0.0 ± 0.0
Met
2.151MetAla: 2.151 ± 0.019
0.252MetCys: 0.252 ± 0.006
1.232MetAsp: 1.232 ± 0.014
1.309MetGlu: 1.309 ± 0.018
0.773MetPhe: 0.773 ± 0.013
1.508MetGly: 1.508 ± 0.019
0.495MetHis: 0.495 ± 0.01
1.063MetIle: 1.063 ± 0.015
0.965MetLys: 0.965 ± 0.012
1.926MetLeu: 1.926 ± 0.019
0.582MetMet: 0.582 ± 0.01
0.805MetAsn: 0.805 ± 0.013
1.222MetPro: 1.222 ± 0.016
0.879MetGln: 0.879 ± 0.014
1.27MetArg: 1.27 ± 0.015
1.897MetSer: 1.897 ± 0.02
1.323MetThr: 1.323 ± 0.016
1.4MetVal: 1.4 ± 0.018
0.275MetTrp: 0.275 ± 0.007
0.575MetTyr: 0.575 ± 0.01
0.0MetXaa: 0.0 ± 0.0
Asn
3.041AsnAla: 3.041 ± 0.022
0.459AsnCys: 0.459 ± 0.011
1.875AsnAsp: 1.875 ± 0.021
1.972AsnGlu: 1.972 ± 0.019
1.333AsnPhe: 1.333 ± 0.015
2.849AsnGly: 2.849 ± 0.024
0.855AsnHis: 0.855 ± 0.013
2.064AsnIle: 2.064 ± 0.019
1.432AsnLys: 1.432 ± 0.016
3.251AsnLeu: 3.251 ± 0.025
0.826AsnMet: 0.826 ± 0.014
1.475AsnAsn: 1.475 ± 0.021
2.445AsnPro: 2.445 ± 0.025
1.301AsnGln: 1.301 ± 0.016
1.946AsnArg: 1.946 ± 0.021
2.614AsnSer: 2.614 ± 0.022
2.172AsnThr: 2.172 ± 0.022
2.337AsnVal: 2.337 ± 0.023
0.583AsnTrp: 0.583 ± 0.01
1.126AsnTyr: 1.126 ± 0.017
0.0AsnXaa: 0.0 ± 0.0
Pro
5.116ProAla: 5.116 ± 0.044
0.553ProCys: 0.553 ± 0.011
3.238ProAsp: 3.238 ± 0.028
3.852ProGlu: 3.852 ± 0.031
2.113ProPhe: 2.113 ± 0.019
3.875ProGly: 3.875 ± 0.035
1.358ProHis: 1.358 ± 0.018
2.578ProIle: 2.578 ± 0.021
2.417ProLys: 2.417 ± 0.023
4.789ProLeu: 4.789 ± 0.032
1.088ProMet: 1.088 ± 0.019
2.161ProAsn: 2.161 ± 0.022
4.645ProPro: 4.645 ± 0.069
2.404ProGln: 2.404 ± 0.03
3.329ProArg: 3.329 ± 0.034
5.943ProSer: 5.943 ± 0.054
3.953ProThr: 3.953 ± 0.033
3.692ProVal: 3.692 ± 0.033
0.799ProTrp: 0.799 ± 0.012
1.604ProTyr: 1.604 ± 0.019
0.0ProXaa: 0.0 ± 0.0
Gln
3.397GlnAla: 3.397 ± 0.032
0.471GlnCys: 0.471 ± 0.01
2.015GlnAsp: 2.015 ± 0.021
2.425GlnGlu: 2.425 ± 0.025
1.321GlnPhe: 1.321 ± 0.017
2.425GlnGly: 2.425 ± 0.022
1.04GlnHis: 1.04 ± 0.013
1.924GlnIle: 1.924 ± 0.02
1.9GlnLys: 1.9 ± 0.021
3.617GlnLeu: 3.617 ± 0.028
0.875GlnMet: 0.875 ± 0.014
1.492GlnAsn: 1.492 ± 0.02
2.506GlnPro: 2.506 ± 0.031
2.346GlnGln: 2.346 ± 0.049
2.586GlnArg: 2.586 ± 0.026
3.167GlnSer: 3.167 ± 0.032
2.31GlnThr: 2.31 ± 0.023
2.246GlnVal: 2.246 ± 0.023
0.613GlnTrp: 0.613 ± 0.011
1.232GlnTyr: 1.232 ± 0.015
0.0GlnXaa: 0.0 ± 0.0
Arg
4.642ArgAla: 4.642 ± 0.033
0.747ArgCys: 0.747 ± 0.012
3.307ArgAsp: 3.307 ± 0.03
3.795ArgGlu: 3.795 ± 0.033
2.224ArgPhe: 2.224 ± 0.024
3.688ArgGly: 3.688 ± 0.031
1.508ArgHis: 1.508 ± 0.018
2.917ArgIle: 2.917 ± 0.022
3.236ArgLys: 3.236 ± 0.03
5.732ArgLeu: 5.732 ± 0.036
1.325ArgMet: 1.325 ± 0.017
2.181ArgAsn: 2.181 ± 0.022
3.414ArgPro: 3.414 ± 0.032
2.609ArgGln: 2.609 ± 0.025
4.929ArgArg: 4.929 ± 0.045
4.721ArgSer: 4.721 ± 0.037
3.26ArgThr: 3.26 ± 0.029
3.551ArgVal: 3.551 ± 0.031
0.976ArgTrp: 0.976 ± 0.011
1.803ArgTyr: 1.803 ± 0.017
0.0ArgXaa: 0.0 ± 0.0
Ser
6.636SerAla: 6.636 ± 0.049
0.905SerCys: 0.905 ± 0.014
4.255SerAsp: 4.255 ± 0.038
4.139SerGlu: 4.139 ± 0.028
3.069SerPhe: 3.069 ± 0.028
5.538SerGly: 5.538 ± 0.04
1.977SerHis: 1.977 ± 0.023
4.025SerIle: 4.025 ± 0.03
3.334SerLys: 3.334 ± 0.031
7.561SerLeu: 7.561 ± 0.037
1.717SerMet: 1.717 ± 0.021
2.921SerAsn: 2.921 ± 0.027
5.312SerPro: 5.312 ± 0.044
3.27SerGln: 3.27 ± 0.029
4.936SerArg: 4.936 ± 0.041
8.761SerSer: 8.761 ± 0.076
5.614SerThr: 5.614 ± 0.041
4.759SerVal: 4.759 ± 0.034
1.194SerTrp: 1.194 ± 0.016
2.203SerTyr: 2.203 ± 0.023
0.0SerXaa: 0.0 ± 0.0
Thr
5.198ThrAla: 5.198 ± 0.04
0.766ThrCys: 0.766 ± 0.015
2.971ThrAsp: 2.971 ± 0.025
3.161ThrGlu: 3.161 ± 0.028
2.24ThrPhe: 2.24 ± 0.02
4.328ThrGly: 4.328 ± 0.034
1.336ThrHis: 1.336 ± 0.015
3.176ThrIle: 3.176 ± 0.025
2.374ThrLys: 2.374 ± 0.022
5.481ThrLeu: 5.481 ± 0.036
1.218ThrMet: 1.218 ± 0.016
2.09ThrAsn: 2.09 ± 0.022
4.184ThrPro: 4.184 ± 0.034
2.075ThrGln: 2.075 ± 0.02
3.045ThrArg: 3.045 ± 0.024
5.276ThrSer: 5.276 ± 0.041
4.439ThrThr: 4.439 ± 0.041
3.959ThrVal: 3.959 ± 0.031
0.896ThrTrp: 0.896 ± 0.015
1.779ThrTyr: 1.779 ± 0.021
0.0ThrXaa: 0.0 ± 0.0
Val
5.294ValAla: 5.294 ± 0.038
0.911ValCys: 0.911 ± 0.016
3.796ValAsp: 3.796 ± 0.03
3.858ValGlu: 3.858 ± 0.036
2.572ValPhe: 2.572 ± 0.025
4.196ValGly: 4.196 ± 0.032
1.47ValHis: 1.47 ± 0.017
3.139ValIle: 3.139 ± 0.028
2.7ValLys: 2.7 ± 0.026
5.878ValLeu: 5.878 ± 0.037
1.42ValMet: 1.42 ± 0.018
2.287ValAsn: 2.287 ± 0.021
3.712ValPro: 3.712 ± 0.027
2.469ValGln: 2.469 ± 0.025
3.624ValArg: 3.624 ± 0.029
4.819ValSer: 4.819 ± 0.033
3.679ValThr: 3.679 ± 0.032
4.49ValVal: 4.49 ± 0.036
0.911ValTrp: 0.911 ± 0.014
1.909ValTyr: 1.909 ± 0.021
0.0ValXaa: 0.0 ± 0.0
Trp
1.173TrpAla: 1.173 ± 0.016
0.218TrpCys: 0.218 ± 0.007
0.928TrpAsp: 0.928 ± 0.013
0.887TrpGlu: 0.887 ± 0.014
0.58TrpPhe: 0.58 ± 0.01
0.997TrpGly: 0.997 ± 0.017
0.363TrpHis: 0.363 ± 0.009
0.81TrpIle: 0.81 ± 0.013
0.813TrpLys: 0.813 ± 0.011
1.441TrpLeu: 1.441 ± 0.018
0.393TrpMet: 0.393 ± 0.008
0.66TrpAsn: 0.66 ± 0.012
0.639TrpPro: 0.639 ± 0.012
0.586TrpGln: 0.586 ± 0.01
1.002TrpArg: 1.002 ± 0.014
1.09TrpSer: 1.09 ± 0.015
0.971TrpThr: 0.971 ± 0.014
0.972TrpVal: 0.972 ± 0.013
0.297TrpTrp: 0.297 ± 0.008
0.464TrpTyr: 0.464 ± 0.009
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.305TyrAla: 2.305 ± 0.022
0.448TyrCys: 0.448 ± 0.01
1.717TyrAsp: 1.717 ± 0.021
1.624TyrGlu: 1.624 ± 0.017
1.27TyrPhe: 1.27 ± 0.016
2.229TyrGly: 2.229 ± 0.025
0.829TyrHis: 0.829 ± 0.014
1.556TyrIle: 1.556 ± 0.018
1.051TyrLys: 1.051 ± 0.015
2.95TyrLeu: 2.95 ± 0.025
0.68TyrMet: 0.68 ± 0.012
1.193TyrAsn: 1.193 ± 0.016
1.673TyrPro: 1.673 ± 0.021
1.164TyrGln: 1.164 ± 0.018
1.747TyrArg: 1.747 ± 0.018
2.16TyrSer: 2.16 ± 0.02
1.762TyrThr: 1.762 ± 0.021
1.784TyrVal: 1.784 ± 0.017
0.504TyrTrp: 0.504 ± 0.01
1.074TyrTyr: 1.074 ± 0.017
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 10949 proteins (5330435 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski