Avon-Heathcote Estuary associated circular virus 29

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Arfiviricetes; Cirlivirales; Circoviridae; unclassified Circoviridae

Average proteome isoelectric point is 8.59

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0C5IMN7|A0A0C5IMN7_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 29 OX=1618253 PE=4 SV=1
MM1 pKa = 7.6SNPITGYY8 pKa = 10.16DD9 pKa = 3.39FRR11 pKa = 11.84YY12 pKa = 10.3NADD15 pKa = 4.09DD16 pKa = 4.1YY17 pKa = 11.32TPQDD21 pKa = 2.52IKK23 pKa = 11.56GFLKK27 pKa = 10.71GVAKK31 pKa = 10.65SFVFQKK37 pKa = 10.77EE38 pKa = 4.28KK39 pKa = 10.95GDD41 pKa = 3.51SGYY44 pKa = 11.14LHH46 pKa = 5.45YY47 pKa = 10.39QGRR50 pKa = 11.84LRR52 pKa = 11.84LIKK55 pKa = 10.04KK56 pKa = 9.56RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84TKK61 pKa = 10.54ALSLFVTPPNYY72 pKa = 9.74FQPTVGSEE80 pKa = 4.21YY81 pKa = 10.86KK82 pKa = 10.85NGDD85 pKa = 3.49AFYY88 pKa = 10.78MMKK91 pKa = 10.27EE92 pKa = 4.01DD93 pKa = 4.05TKK95 pKa = 10.95IEE97 pKa = 4.29GPWTDD102 pKa = 3.13KK103 pKa = 11.78DD104 pKa = 3.79EE105 pKa = 4.78VKK107 pKa = 11.03VLTTQLAWYY116 pKa = 8.9NSQEE120 pKa = 3.76EE121 pKa = 4.45RR122 pKa = 11.84PFQKK126 pKa = 10.34KK127 pKa = 9.09LRR129 pKa = 11.84EE130 pKa = 3.95MCSIFDD136 pKa = 3.72MRR138 pKa = 11.84TIDD141 pKa = 5.88LIWDD145 pKa = 3.6TTGNCGKK152 pKa = 10.46SLLCEE157 pKa = 3.77ALEE160 pKa = 3.87YY161 pKa = 11.0DD162 pKa = 4.49GLCEE166 pKa = 4.57EE167 pKa = 4.44IPSYY171 pKa = 11.4RR172 pKa = 11.84LMDD175 pKa = 6.8DD176 pKa = 3.46IFQWVATRR184 pKa = 11.84PIKK187 pKa = 10.29KK188 pKa = 9.74CYY190 pKa = 8.94VVDD193 pKa = 3.78MPRR196 pKa = 11.84GMKK199 pKa = 9.65KK200 pKa = 10.18DD201 pKa = 3.54RR202 pKa = 11.84LGDD205 pKa = 4.07FYY207 pKa = 11.73SGIEE211 pKa = 4.05VIKK214 pKa = 11.17NGVAYY219 pKa = 10.12DD220 pKa = 3.64KK221 pKa = 10.52RR222 pKa = 11.84YY223 pKa = 8.59HH224 pKa = 5.83AKK226 pKa = 9.62KK227 pKa = 10.48VRR229 pKa = 11.84FSRR232 pKa = 11.84PRR234 pKa = 11.84IFVFTNTLPCLNLMSIDD251 pKa = 3.04RR252 pKa = 11.84WKK254 pKa = 9.89IWKK257 pKa = 9.95VNEE260 pKa = 3.91NYY262 pKa = 10.49EE263 pKa = 4.87LIPHH267 pKa = 6.84HH268 pKa = 6.67

Molecular weight: 31.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0C5IMN7|A0A0C5IMN7_9CIRC Replication-associated protein OS=Avon-Heathcote Estuary associated circular virus 29 OX=1618253 PE=4 SV=1
MM1 pKa = 7.35SNTWGGLQGLAPSTIQGTGAEE22 pKa = 4.38SSMTTYY28 pKa = 10.77GKK30 pKa = 10.02NRR32 pKa = 11.84IGNEE36 pKa = 2.93INMRR40 pKa = 11.84HH41 pKa = 5.37WSMDD45 pKa = 3.43CFIDD49 pKa = 5.3LPKK52 pKa = 10.68ALNGTPTFPLSQIPCRR68 pKa = 11.84IILADD73 pKa = 3.66NLTDD77 pKa = 5.38DD78 pKa = 5.27GALQAQDD85 pKa = 3.94VLQNPLSNAQSLISPYY101 pKa = 10.32KK102 pKa = 10.22NSANASKK109 pKa = 10.36RR110 pKa = 11.84YY111 pKa = 9.68KK112 pKa = 10.16IYY114 pKa = 10.74ADD116 pKa = 3.41YY117 pKa = 11.06KK118 pKa = 9.74FTVAGNKK125 pKa = 8.93AGKK128 pKa = 9.64RR129 pKa = 11.84IHH131 pKa = 6.68FKK133 pKa = 10.48MPIPKK138 pKa = 9.56SGRR141 pKa = 11.84VVHH144 pKa = 5.69YY145 pKa = 10.42QDD147 pKa = 4.63GASSTPSDD155 pKa = 3.54FNMSVLFFAEE165 pKa = 4.39ISPAGTNFPNLNLVVKK181 pKa = 10.7SRR183 pKa = 11.84FTDD186 pKa = 3.06AA187 pKa = 4.86

Molecular weight: 20.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

455

187

268

227.5

26.03

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.495 ± 1.859

1.758 ± 0.418

6.593 ± 0.757

3.956 ± 1.428

5.055 ± 0.147

6.593 ± 0.542

1.538 ± 0.04

6.154 ± 0.16

8.132 ± 1.366

7.473 ± 0.009

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.297 ± 0.054

5.714 ± 1.401

5.495 ± 0.56

3.516 ± 0.463

5.495 ± 1.063

6.593 ± 1.841

5.934 ± 0.293

4.615 ± 0.53

1.758 ± 0.418

4.835 ± 0.988
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski