Sanguibacter gelidistatuariae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Sanguibacteraceae; Sanguibacter

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3525 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G6QEP6|A0A1G6QEP6_9MICO NADH dehydrogenase OS=Sanguibacter gelidistatuariae OX=1814289 GN=SAMN05216410_2524 PE=4 SV=1
MM1 pKa = 7.07NRR3 pKa = 11.84SSVRR7 pKa = 11.84AWAAAASAVLVTGIGLGGCSFVTGTTDD34 pKa = 2.55IVQLVGVAMPTTTSLRR50 pKa = 11.84WIEE53 pKa = 4.57DD54 pKa = 3.42GNNVKK59 pKa = 10.5AQLEE63 pKa = 4.22DD64 pKa = 2.93LGYY67 pKa = 10.89AVEE70 pKa = 5.09LVYY73 pKa = 11.14AEE75 pKa = 4.44NDD77 pKa = 3.25VPTQVAQVEE86 pKa = 4.34AMISHH91 pKa = 6.81GADD94 pKa = 3.18LLIIGSVDD102 pKa = 2.95GTALKK107 pKa = 10.65DD108 pKa = 3.7QLASAAAAGIPIISYY123 pKa = 10.58DD124 pKa = 3.42RR125 pKa = 11.84LIRR128 pKa = 11.84DD129 pKa = 3.86SPDD132 pKa = 2.49VDD134 pKa = 4.09YY135 pKa = 11.58YY136 pKa = 11.54ATFDD140 pKa = 3.62NLRR143 pKa = 11.84VGTQQATSLLQGLGVLDD160 pKa = 4.15AAGEE164 pKa = 4.24PTGAAGPFAIEE175 pKa = 4.22VFAGSPDD182 pKa = 3.96DD183 pKa = 4.44NNATVFYY190 pKa = 9.36EE191 pKa = 4.32GAFAVLQPYY200 pKa = 9.48IDD202 pKa = 3.98SGVLVIPSGEE212 pKa = 4.06TDD214 pKa = 3.71FQTIAIQAWDD224 pKa = 3.69PVLGGEE230 pKa = 5.33RR231 pKa = 11.84MDD233 pKa = 4.57ALLSTTYY240 pKa = 10.08TSSTEE245 pKa = 4.16VIDD248 pKa = 5.96GILSPYY254 pKa = 10.39DD255 pKa = 3.96GISRR259 pKa = 11.84AVIEE263 pKa = 4.27SLKK266 pKa = 8.58TAGYY270 pKa = 7.85GTGDD274 pKa = 3.33RR275 pKa = 11.84PLPVVSGQDD284 pKa = 3.11AEE286 pKa = 4.5LPSVQSIIAGEE297 pKa = 4.17QYY299 pKa = 9.62STIYY303 pKa = 10.71KK304 pKa = 8.42DD305 pKa = 3.38TRR307 pKa = 11.84QLAEE311 pKa = 3.92VTVAMGDD318 pKa = 3.57ALLKK322 pKa = 10.35GAEE325 pKa = 4.49PEE327 pKa = 4.44TNDD330 pKa = 2.72ITSYY334 pKa = 11.74DD335 pKa = 3.88NGTGIVPTYY344 pKa = 10.9LLGPLVVTQATYY356 pKa = 11.11QSILVGGGYY365 pKa = 7.89YY366 pKa = 9.74TEE368 pKa = 5.39DD369 pKa = 3.61EE370 pKa = 4.38LRR372 pKa = 4.97

Molecular weight: 38.92 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G6XGV6|A0A1G6XGV6_9MICO Evolved beta-galactosidase subunit beta OS=Sanguibacter gelidistatuariae OX=1814289 GN=SAMN05216410_0131 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3525

0

3525

1210563

25

2253

343.4

36.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.728 ± 0.06

0.583 ± 0.012

6.14 ± 0.033

5.169 ± 0.035

2.875 ± 0.023

9.024 ± 0.039

2.031 ± 0.021

4.13 ± 0.032

1.899 ± 0.026

10.159 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.844 ± 0.018

1.879 ± 0.021

5.588 ± 0.029

2.834 ± 0.021

6.823 ± 0.043

5.895 ± 0.03

6.684 ± 0.042

9.344 ± 0.038

1.473 ± 0.018

1.897 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski