Edwardsiella phage GF-2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Gofduovirus; Edwardsiella virus GF2

Average proteome isoelectric point is 6.34

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A077K9Y1|A0A077K9Y1_9CAUD Putative DNA-binding protein Roi OS=Edwardsiella phage GF-2 OX=1537091 PE=4 SV=1
MM1 pKa = 7.38NMRR4 pKa = 11.84EE5 pKa = 4.18EE6 pKa = 4.13FEE8 pKa = 4.26KK9 pKa = 10.66WLTNCLGPFVDD20 pKa = 4.47MSRR23 pKa = 11.84DD24 pKa = 3.27VDD26 pKa = 3.85DD27 pKa = 6.56GVYY30 pKa = 9.62EE31 pKa = 4.09WQDD34 pKa = 3.07VRR36 pKa = 11.84LAWLAWKK43 pKa = 9.56AAQPEE48 pKa = 4.39WIEE51 pKa = 4.23RR52 pKa = 11.84AEE54 pKa = 4.05HH55 pKa = 5.88TPVDD59 pKa = 4.24SKK61 pKa = 10.88WYY63 pKa = 10.09VVSTYY68 pKa = 10.06YY69 pKa = 10.73GYY71 pKa = 11.1YY72 pKa = 7.8IQCWSDD78 pKa = 3.28GQGWLGDD85 pKa = 3.98DD86 pKa = 3.85VSIADD91 pKa = 3.95GDD93 pKa = 4.14VTHH96 pKa = 7.42LIPLPTPPEE105 pKa = 3.81AA106 pKa = 5.44

Molecular weight: 12.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A077KAZ8|A0A077KAZ8_9CAUD Uncharacterized protein OS=Edwardsiella phage GF-2 OX=1537091 PE=4 SV=1
MM1 pKa = 7.5AKK3 pKa = 8.92STDD6 pKa = 3.4VHH8 pKa = 6.84DD9 pKa = 5.92LLIAYY14 pKa = 7.54QKK16 pKa = 9.24QARR19 pKa = 11.84KK20 pKa = 9.49IPAKK24 pKa = 10.14GVYY27 pKa = 9.82ASRR30 pKa = 11.84NRR32 pKa = 11.84QVEE35 pKa = 4.19VQTAHH40 pKa = 5.17TRR42 pKa = 11.84KK43 pKa = 9.91LMRR46 pKa = 11.84KK47 pKa = 8.36RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84SVGKK54 pKa = 9.79SNKK57 pKa = 9.85LGFRR61 pKa = 11.84FAAEE65 pKa = 3.92MRR67 pKa = 11.84VSLICDD73 pKa = 3.22MNFWALVCRR82 pKa = 11.84SNRR85 pKa = 11.84HH86 pKa = 4.4NTNLTNEE93 pKa = 4.2VVVIGMEE100 pKa = 4.42GEE102 pKa = 3.97

Molecular weight: 11.73 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

13428

38

568

163.8

18.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.458 ± 0.344

1.199 ± 0.125

6.032 ± 0.279

6.39 ± 0.43

3.441 ± 0.18

7.216 ± 0.401

1.34 ± 0.132

6.464 ± 0.204

5.913 ± 0.306

7.551 ± 0.28

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.867 ± 0.157

4.52 ± 0.221

4.021 ± 0.187

4.155 ± 0.218

5.593 ± 0.34

7.172 ± 0.4

5.489 ± 0.253

6.27 ± 0.283

1.609 ± 0.152

3.299 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski