Aphanomyces astaci

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Oomycota; Saprolegniales; Saprolegniaceae; Aphanomyces

Average proteome isoelectric point is 6.78

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 24980 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|W4H0V3|W4H0V3_9STRA CHK domain-containing protein OS=Aphanomyces astaci OX=112090 GN=B5M09_006205 PE=4 SV=1
MM1 pKa = 7.3NALHH5 pKa = 6.69LADD8 pKa = 3.98VNGTTGAPVLDD19 pKa = 4.26ADD21 pKa = 4.08EE22 pKa = 5.14HH23 pKa = 7.11VLATFLNVRR32 pKa = 11.84MYY34 pKa = 10.86TNTYY38 pKa = 9.52GSNDD42 pKa = 4.38DD43 pKa = 5.26DD44 pKa = 4.55DD45 pKa = 6.03ASPSMEE51 pKa = 4.13SKK53 pKa = 9.84GTGNLYY59 pKa = 8.51VTSARR64 pKa = 11.84VAWIFGQEE72 pKa = 4.01SEE74 pKa = 4.76GVVGYY79 pKa = 10.47AWDD82 pKa = 3.55MTFLSLHH89 pKa = 6.61AISRR93 pKa = 11.84DD94 pKa = 3.23TSSFPEE100 pKa = 4.13PCLYY104 pKa = 10.6CQLDD108 pKa = 3.26VDD110 pKa = 5.1DD111 pKa = 5.17EE112 pKa = 4.76VNEE115 pKa = 4.05IRR117 pKa = 11.84FVPCDD122 pKa = 3.2VDD124 pKa = 3.64KK125 pKa = 11.16QLQAMFDD132 pKa = 3.59AFSASAALNPDD143 pKa = 4.46DD144 pKa = 6.54DD145 pKa = 7.03DD146 pKa = 6.88DD147 pKa = 6.3DD148 pKa = 4.49EE149 pKa = 4.85PQGGDD154 pKa = 2.9WIYY157 pKa = 11.61NEE159 pKa = 4.54DD160 pKa = 3.77EE161 pKa = 4.48VVNGARR167 pKa = 11.84EE168 pKa = 4.17ANLAAHH174 pKa = 7.18FDD176 pKa = 4.1SILQVVPSLDD186 pKa = 3.63ANVAGQFDD194 pKa = 4.34DD195 pKa = 5.49ASDD198 pKa = 4.34DD199 pKa = 3.73EE200 pKa = 4.74SLLL203 pKa = 3.93

Molecular weight: 22.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|W4HCP3|W4HCP3_9STRA PIPK domain-containing protein OS=Aphanomyces astaci OX=112090 GN=H257_00873 PE=4 SV=1
MM1 pKa = 6.92QLKK4 pKa = 10.15HH5 pKa = 6.38PSRR8 pKa = 11.84PKK10 pKa = 10.58HH11 pKa = 6.06SMQPSRR17 pKa = 11.84PFQSPHH23 pKa = 6.26RR24 pKa = 11.84PVGRR28 pKa = 11.84AQLRR32 pKa = 11.84GRR34 pKa = 11.84RR35 pKa = 11.84PPLLGLQPPSPPRR48 pKa = 11.84RR49 pKa = 3.51

Molecular weight: 5.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19020

5960

24980

11550940

31

12913

462.4

51.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.303 ± 0.016

1.809 ± 0.009

5.903 ± 0.013

4.723 ± 0.015

3.642 ± 0.01

5.649 ± 0.018

3.344 ± 0.012

3.967 ± 0.01

4.457 ± 0.017

9.695 ± 0.019

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.531 ± 0.006

3.304 ± 0.01

5.505 ± 0.017

4.176 ± 0.01

5.774 ± 0.016

7.845 ± 0.017

6.606 ± 0.017

7.815 ± 0.017

1.374 ± 0.007

2.577 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski