Marinobacter phage PS3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 54 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2D1GMR0|A0A2D1GMR0_9CAUD Uncharacterized protein OS=Marinobacter phage PS3 OX=2041341 PE=4 SV=1
MM1 pKa = 7.75ASILGKK7 pKa = 10.64LGIQEE12 pKa = 4.22GDD14 pKa = 3.58SYY16 pKa = 11.16TAAAGSNRR24 pKa = 11.84AVVVAGTGEE33 pKa = 4.22SAFSSRR39 pKa = 11.84EE40 pKa = 3.51IVGITFGGVAMTRR53 pKa = 11.84AAYY56 pKa = 9.13TGDD59 pKa = 3.4TEE61 pKa = 4.34TAKK64 pKa = 11.02GGVYY68 pKa = 10.35YY69 pKa = 9.99ILEE72 pKa = 4.24SEE74 pKa = 4.38IPAGSSVIAVDD85 pKa = 3.06WDD87 pKa = 4.15GQVHH91 pKa = 6.65DD92 pKa = 5.02AFTGAVYY99 pKa = 9.74TLGGIDD105 pKa = 3.87QVTPVDD111 pKa = 3.93NTNSAIDD118 pKa = 3.52VGQFNLDD125 pKa = 3.15ITFTGVDD132 pKa = 3.48GGVLIVGAGSNSPADD147 pKa = 3.83TLNQSSTISATTTTVTVDD165 pKa = 3.32HH166 pKa = 6.41AQITAGHH173 pKa = 6.52EE174 pKa = 4.18GSAISGVLSAAGSEE188 pKa = 4.43TLGLRR193 pKa = 11.84YY194 pKa = 9.81SSEE197 pKa = 4.03TDD199 pKa = 3.25PVGALAVFNSSGAGPSGPSLDD220 pKa = 4.17TVPSTAYY227 pKa = 9.03PGQSRR232 pKa = 11.84TVSGSGFGATQGTGGLTIGGVSQTITSWSDD262 pKa = 2.83TSITFTAVLGANSYY276 pKa = 11.28GSGKK280 pKa = 8.66TIEE283 pKa = 4.29VTTDD287 pKa = 3.74DD288 pKa = 5.42DD289 pKa = 4.17DD290 pKa = 4.28TDD292 pKa = 3.67SGTIEE297 pKa = 4.7LVADD301 pKa = 3.6TAGGFGVVTMSSPNTADD318 pKa = 3.44EE319 pKa = 4.36SSVAYY324 pKa = 9.88GATPTVVTGDD334 pKa = 3.34QFEE337 pKa = 4.57WEE339 pKa = 4.63DD340 pKa = 4.7FNDD343 pKa = 4.18LGNLAIGSDD352 pKa = 3.73AFVSSVDD359 pKa = 3.39TEE361 pKa = 4.34GTFRR365 pKa = 11.84GRR367 pKa = 11.84FWDD370 pKa = 4.15ASDD373 pKa = 3.99GTWGSVNTFTASATDD388 pKa = 3.5EE389 pKa = 4.21TAPTISSASVPTAGNNIAVQMDD411 pKa = 3.14EE412 pKa = 4.14SMQVGAGGTGGWTISLAGVSVSSASVDD439 pKa = 3.48GSDD442 pKa = 3.39DD443 pKa = 3.65TVVNLTPSRR452 pKa = 11.84TLTDD456 pKa = 3.63EE457 pKa = 4.13DD458 pKa = 4.4TLAIGYY464 pKa = 6.09TQPGDD469 pKa = 3.73GFQDD473 pKa = 3.73QADD476 pKa = 4.38TPNDD480 pKa = 3.51LATLSGQAVTNNSAQEE496 pKa = 4.16PPDD499 pKa = 3.12VTGPVTEE506 pKa = 4.59SVDD509 pKa = 3.53VPTGGTYY516 pKa = 10.4AIGDD520 pKa = 3.94DD521 pKa = 5.22LSFTVNWDD529 pKa = 3.23EE530 pKa = 4.12AVTVTGTPALNLDD543 pKa = 3.09IGGTSRR549 pKa = 11.84QADD552 pKa = 3.93YY553 pKa = 11.45ASGSGSSALVFTYY566 pKa = 9.35TVQSGDD572 pKa = 3.48EE573 pKa = 4.41DD574 pKa = 4.8DD575 pKa = 4.93NGIAVSSLTLEE586 pKa = 4.55GGTLQDD592 pKa = 3.58ASSNNATLTLNSVGDD607 pKa = 3.8TSGVLVDD614 pKa = 4.9GVAPVISINALTTTDD629 pKa = 3.46TTPVVTGSAEE639 pKa = 4.1DD640 pKa = 3.89ADD642 pKa = 4.57SLVLDD647 pKa = 4.66LEE649 pKa = 4.87GVDD652 pKa = 3.55ITHH655 pKa = 6.51SSSYY659 pKa = 10.9NITPSAGSWSQQLPEE674 pKa = 4.37LALGEE679 pKa = 4.2YY680 pKa = 10.79LMTLTGVDD688 pKa = 3.17SAGNEE693 pKa = 4.34TVVTAALNVVEE704 pKa = 5.54SVATSPRR711 pKa = 11.84GLFQPLFRR719 pKa = 11.84SLSGSVNQTLFRR731 pKa = 5.91

Molecular weight: 73.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2D1GN28|A0A2D1GN28_9CAUD Uncharacterized protein OS=Marinobacter phage PS3 OX=2041341 PE=4 SV=1
MM1 pKa = 7.88DD2 pKa = 5.64RR3 pKa = 11.84FTATEE8 pKa = 4.16FTAINKK14 pKa = 9.79DD15 pKa = 3.16GRR17 pKa = 11.84LTRR20 pKa = 11.84AEE22 pKa = 4.57LEE24 pKa = 4.06TLAWMSEE31 pKa = 3.9GKK33 pKa = 10.12EE34 pKa = 3.76NAAIGILRR42 pKa = 11.84GHH44 pKa = 6.32GTPGAKK50 pKa = 9.94KK51 pKa = 9.98LVSNVMLKK59 pKa = 10.27FNANSRR65 pKa = 11.84CLAIARR71 pKa = 11.84ACRR74 pKa = 11.84DD75 pKa = 3.49GFVVAAAKK83 pKa = 10.04SRR85 pKa = 11.84HH86 pKa = 4.97IAAAFLILASSWVSTTLPDD105 pKa = 3.05SDD107 pKa = 4.07VYY109 pKa = 11.08RR110 pKa = 11.84RR111 pKa = 11.84GANRR115 pKa = 11.84STSVRR120 pKa = 11.84LGPRR124 pKa = 11.84GPGRR128 pKa = 11.84LEE130 pKa = 3.36MDD132 pKa = 4.07LNNWLGAAA140 pKa = 4.27

Molecular weight: 15.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

54

0

54

12528

45

911

232.0

25.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.954 ± 0.482

0.894 ± 0.127

6.489 ± 0.258

6.657 ± 0.272

3.52 ± 0.214

8.517 ± 0.313

1.716 ± 0.185

4.829 ± 0.306

4.27 ± 0.357

8.565 ± 0.264

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.083 ± 0.15

3.736 ± 0.19

4.502 ± 0.307

4.215 ± 0.172

5.819 ± 0.414

6.992 ± 0.405

6.186 ± 0.435

6.745 ± 0.301

1.557 ± 0.165

2.754 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski