Bacillus phage vB_BspS_SplendidRed

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.68

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 76 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5B9NKX7|A0A5B9NKX7_9CAUD Uncharacterized protein OS=Bacillus phage vB_BspS_SplendidRed OX=2591379 GN=SPLENDIDRED_71 PE=4 SV=1
MM1 pKa = 7.73AEE3 pKa = 4.13KK4 pKa = 10.64ALDD7 pKa = 3.66KK8 pKa = 10.53PIQGKK13 pKa = 10.19DD14 pKa = 2.9ILYY17 pKa = 10.21LIQSVDD23 pKa = 3.51AEE25 pKa = 4.66LGASPLFPAFQTDD38 pKa = 4.58GSFSWEE44 pKa = 3.4NDD46 pKa = 3.09LFDD49 pKa = 4.13EE50 pKa = 4.27QTKK53 pKa = 10.14NGRR56 pKa = 11.84VLGYY60 pKa = 10.47GGDD63 pKa = 3.78SEE65 pKa = 5.89SIEE68 pKa = 3.53FTYY71 pKa = 10.67YY72 pKa = 10.86GKK74 pKa = 10.34QGDD77 pKa = 4.07EE78 pKa = 4.08GQEE81 pKa = 4.06TIEE84 pKa = 4.0TVIEE88 pKa = 4.15KK89 pKa = 10.16KK90 pKa = 9.91QQLKK94 pKa = 8.25VWRR97 pKa = 11.84VNRR100 pKa = 11.84NKK102 pKa = 10.56NADD105 pKa = 3.47GKK107 pKa = 10.6FDD109 pKa = 3.61ARR111 pKa = 11.84FAYY114 pKa = 10.2VLIEE118 pKa = 3.88SRR120 pKa = 11.84EE121 pKa = 4.1YY122 pKa = 11.3NDD124 pKa = 4.16GADD127 pKa = 3.42GSVEE131 pKa = 3.86ISISTQVLGLSKK143 pKa = 10.59KK144 pKa = 10.5GVIDD148 pKa = 3.88SLPDD152 pKa = 4.0EE153 pKa = 5.01ILNAANGGYY162 pKa = 9.82EE163 pKa = 3.93FQAPGEE169 pKa = 4.48TTGEE173 pKa = 4.4SPGEE177 pKa = 4.23TQVTPP182 pKa = 3.81

Molecular weight: 19.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5B9NL54|A0A5B9NL54_9CAUD Uncharacterized protein OS=Bacillus phage vB_BspS_SplendidRed OX=2591379 GN=SPLENDIDRED_8 PE=4 SV=1
MM1 pKa = 7.04YY2 pKa = 10.41SGVEE6 pKa = 4.09FSRR9 pKa = 11.84TGVRR13 pKa = 11.84KK14 pKa = 8.48MARR17 pKa = 11.84NQTKK21 pKa = 10.1KK22 pKa = 10.87GKK24 pKa = 9.98

Molecular weight: 2.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

76

0

76

13804

24

1084

181.6

20.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.136 ± 0.544

0.833 ± 0.151

6.114 ± 0.347

7.802 ± 0.6

3.876 ± 0.151

7.128 ± 0.306

1.702 ± 0.14

6.817 ± 0.241

8.853 ± 0.381

7.128 ± 0.255

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.187 ± 0.228

5.201 ± 0.201

2.934 ± 0.182

3.253 ± 0.148

5.143 ± 0.286

5.687 ± 0.358

5.832 ± 0.31

6.382 ± 0.273

1.413 ± 0.174

3.579 ± 0.301
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski